排序方式: 共有120条查询结果,搜索用时 15 毫秒
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Richardson LJ Kaestli M Mayo M Bowers JR Tuanyok A Schupp J Engelthaler D Wagner DM Keim PS Currie BJ 《Journal of microbiological methods》2012,88(1):179-181
Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat. 相似文献
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Chadia L. Robertson Jyoti Srivastava Ayesha Siddiq Rachel Gredler Luni Emdad Devaraja Rajasekaran Maaged Akiel Xue-Ning Shen Frank Corwin Gobalakrishnan Sundaresan Jamal Zweit Colleen Croniger Xiaoli Gao Shobha Ghosh Philip B. Hylemon Mark A. Subler Jolene J. Windle Paul B. Fisher Devanand Sarkar 《The Journal of biological chemistry》2015,290(29):18227-18236
Astrocyte elevated gene-1 (AEG-1), also known as MTDH (metadherin) or LYRIC, is an established oncogene. However, the physiological function of AEG-1 is not known. To address this question, we generated an AEG-1 knock-out mouse (AEG-1KO) and characterized it. Although AEG-1KO mice were viable and fertile, they were significantly leaner with prominently less body fat and lived significantly longer compared with wild type (WT). When fed a high fat and cholesterol diet (HFD), WT mice rapidly gained weight, whereas AEG-1KO mice did not gain weight at all. This phenotype of AEG-1KO mice is due to decreased fat absorption from the intestines, not because of decreased fat synthesis or increased fat consumption. AEG-1 interacts with retinoid X receptor (RXR) and inhibits RXR function. In enterocytes of AEG-1KO mice, we observed increased activity of RXR heterodimer partners, liver X receptor and peroxisome proliferator-activated receptor-α, key inhibitors of intestinal fat absorption. Inhibition of fat absorption in AEG-1KO mice was further augmented when fed an HFD providing ligands to liver X receptor and peroxisome proliferator-activated receptor-α. Our studies reveal a novel role of AEG-1 in regulating nuclear receptors controlling lipid metabolism. AEG-1 may significantly modulate the effects of HFD and thereby function as a unique determinant of obesity. 相似文献
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Reciprocal translocation of small numbers of inbred individuals rescues immunogenetic diversity 下载免费PDF全文
Catherine E. Grueber Jolene T. Sutton Sol Heber James V. Briskie Bruce C. Robertson 《Molecular ecology》2017,26(10):2660-2673
Genetic rescue can reduce inbreeding depression and increase fitness of small populations, even when the donor populations are highly inbred. In a recent experiment involving two inbred island populations of the New Zealand South Island robin, Petroica australis, reciprocal translocations improved microsatellite diversity and individual fitness. While microsatellite loci may reflect patterns of genome‐wide diversity, they generally do not indicate the specific genetic regions responsible for increased fitness. We tested the effectiveness of this reciprocal translocation for rescuing diversity of two immunogenetic regions: Toll‐like receptor (TLR) and major histocompatibility complex (MHC) genes. We found that the relatively small number of migrants (seven and ten per island) effectively brought the characteristic TLR gene diversity of each source population into the recipient population. However, when migrants transmitted TLR alleles that were already present at high frequency in the recipient population, it was possible for offspring of mixed heritage to have decreased gene diversity compared to recipient population diversity prior to translocation. In contrast to TLRs, we did not observe substantial changes in MHC allelic diversity following translocation, with limited evidence of a decrease in differentiation, perhaps because most MHC alleles were observed at both sites prior to the translocation. Overall, we conclude that small numbers of migrants may successfully restore the diversity of immunogenetic loci with few alleles, but that translocating larger numbers of animals would provide additional opportunity for the genetic rescue of highly polymorphic immunity regions, such as the MHC, even when the source population is inbred. 相似文献
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Liguori AP Warrington SD Ginther JL Pearson T Bowers J Glass MB Mayo M Wuthiekanun V Engelthaler D Peacock SJ Currie BJ Wagner DM Keim P Tuanyok A 《PloS one》2011,6(12):e29323
Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species. 相似文献
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Burger MT Hiebert C Seid M Chu DT Barker L Langhorne M Shawar R Kidney J Desai MC Plattner JJ 《Bioorganic & medicinal chemistry》2006,14(16):5592-5604
A novel series of C(12) ethyl erythromycin derivatives have been discovered which exhibit in vitro and in vivo potency against key respiratory pathogens, including those resistant to erythromycin. The C(12) modification involves replacing the natural C(12) methyl group in the erythromycin core with an ethyl group via chemical synthesis. From the C(12) ethyl macrolide core, a series of C(12) ethyl ketolides were prepared and tested for antibacterial activity against a panel of relevant clinical isolates. Several compounds were found to be potent against macrolide-sensitive and -resistant bacteria, whether resistance was due to ribosome methylation (erm) or efflux (mef). In particular, the C(12) ethyl ketolides 4k,4s,4q,4m, and 4t showed a similar antimicrobial spectrum and comparable activity to the commercial ketolide telithromycin. The in vivo efficacy of several C(12) ethyl ketolides was demonstrated in a mouse infection model with Streptococcus pneumoniae as pathogen. 相似文献
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Predators may affect prey population growth and community diversity through density mediated lethal and trait mediated non-lethal effects that influence phenotypic traits of prey. We tested experimentally the roles of thinning the density of prey (lethality) in the absence of predator cues and density and trait mediated effects (lethality + intimidation) of predatory midge Corethrella appendiculata on competing native and invasive mosquito prey. Predator-mediated reductions in prey and density reductions in the absence of C. appendiculata resulted in lower percent survivorship to adulthood and estimates of the finite rate of increase (λ′) for invasive mosquito Aedes albopictus relative to that of controls. In most instances, thinning the density of prey in the absence, but not in the presence, of C. appendiculata cues resulted in lower survivorship to adulthood and λ′ for native mosquito Aedes triseriatus relative to that of controls. Together, these results suggested trait mediated effects of C. appendiculata specific to each species of mosquito prey. Release from intraspecific competition attributable to density reductions in the absence, but not in the presence, of C. appendiculata enhanced growth and lengthened adult lifespan relative to that of controls for A. albopictus but not A. triseriatus. These results show the importance of predator-mediated density and trait mediated effects on phenotypic traits and populations of invasive and native mosquitoes. Species-specific differences in the phenotypic responses of prey may be due, in part, to longer evolutionary history of C. appendiculata with A. triseriatus than A. albopictus. 相似文献
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Catherine M. Phillips Louisa Goumidi Sandrine Bertrais Martyn R. Field L. Adrienne Cupples Jose M. Ordovas Jolene McMonagle Catherine Defoort Julie A. Lovegrove Christian A. Drevon Ellen E. Blaak Beata Kiec-Wilk Ulf Riserus Jose Lopez-Miranda Ross McManus Serge Hercberg Denis Lairon Richard Planells Helen M. Roche 《Journal of lipid research》2010,51(12):3500-3507
Acetyl-CoA carboxylase β (ACC2) plays a key role in fatty acid synthesis and oxidation pathways. Disturbance of these pathways is associated with impaired insulin responsiveness and metabolic syndrome (MetS). Gene-nutrient interactions may affect MetS risk. This study determined the relationship between ACC2 polymorphisms (rs2075263, rs2268387, rs2284685, rs2284689, rs2300453, rs3742023, rs3742026, rs4766587, and rs6606697) and MetS risk, and whether dietary fatty acids modulate this in the LIPGENE-SU.VI.MAX study of MetS cases and matched controls (n = 1754). Minor A allele carriers of rs4766587 had increased MetS risk (OR 1.29 [CI 1.08, 1.58], P = 0.0064) compared with the GG homozygotes, which may in part be explained by their increased body mass index (BMI), abdominal obesity, and impaired insulin sensitivity (P < 0.05). MetS risk was modulated by dietary fat intake (P = 0.04 for gene-nutrient interaction), where risk conferred by the A allele was exacerbated among individuals with a high-fat intake (>35% energy) (OR 1.62 [CI 1.05, 2.50], P = 0.027), particularly a high intake (>5.5% energy) of n-6 polyunsaturated fat (PUFA) (OR 1.82 [CI 1.14, 2.94], P = 0.01; P = 0.05 for gene-nutrient interaction). Saturated and monounsaturated fat intake did not modulate MetS risk. Importantly, we replicated some of these findings in an independent cohort. In conclusion, the ACC2 rs4766587 polymorphism influences MetS risk, which was modulated by dietary fat, suggesting novel gene-nutrient interactions. 相似文献