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101.
Because of their decreased overall fitness and genetic variability inbred individuals are expected to show reduced survival and lifespan under most environmental conditions as compared with outbred individuals. Whereas evidence for the deleterious effects of inbreeding on lifespan has been previously provided, only a few studies have investigated effects of inbreeding on survival under starved conditions. In the present study we compared the abilities of inbred and outbred adult Drosophila melanogaster to survive under starved and fed conditions. We found that inbreeding reduced lifespan but had no effect on starvation resistance. The results indicate highly trait specific consequences of inbreeding. Possible mechanisms behind the observed results are discussed. 相似文献
102.
Krister Melén Markku Partinen Janne Tynell Maarit Sillanp?? Sari-Leena Himanen Outi Saarenp??-Heikkil? Christer Hublin P?ivi Olsen Jorma Ilonen Hanna Nohynek Ritva Syrj?nen Terhi Kilpi Arja Vuorela Turkka Kirjavainen Outi Vaarala Ilkka Julkunen 《PloS one》2013,8(8)
Background
Narcolepsy cataplexy syndrome, characterised by excessive daytime sleepiness and cataplexy, is strongly associated with a genetic marker, human leukocyte antigen (HLA) DQB1*06:02. A sudden increase in the incidence of childhood narcolepsy was observed after vaccination with AS03-adjuvanted Pandemrix influenza vaccine in Finland at the beginning of 2010. Here, we analysed whether the coinciding influenza A H1N1pdm pandemic contributed, together with the Pandemrix vaccination, to the increased incidence of childhood narcolepsy in 2010. The analysis was based on the presence or absence of antibody response against non-structural protein 1 (NS1) from H1N1pdm09 virus, which was not a component of Pandemrix vaccine.Methods
Non-structural (NS) 1 proteins from recombinant influenza A/Udorn/72 (H3N2) and influenza A/Finland/554/09 (H1N1pdm09) viruses were purified and used in Western blot analysis to determine specific antibody responses in human sera. The sera were obtained from 45 patients who fell ill with narcolepsy after vaccination with AS03-adjuvanted Pandemrix at the end of 2009, and from controls.Findings
Based on quantitative Western blot analysis, only two of the 45 (4.4%) Pandemrix-vaccinated narcoleptic patients showed specific antibody response against the NS1 protein from the H1N1pdm09 virus, indicating past infection with the H1N1pdm09 virus. Instead, paired serum samples from patients, who suffered from a laboratory confirmed H1N1pdm09 infection, showed high levels or diagnostic rises (96%) in H1N1pdm virus NS1-specific antibodies and very high cross-reactivity to H3N2 subtype influenza A virus NS1 protein.Conclusion
Based on our findings, it is unlikely that H1N1pdm09 virus infection contributed to a sudden increase in the incidence of childhood narcolepsy observed in Finland in 2010 after AS03-adjuvanted Pandemrix vaccination. 相似文献103.
Terhi Vuorinen Anne-Marja Nerg Leena Syrjälä Petri Peltonen Jarmo K. Holopainen 《Arthropod-Plant Interactions》2007,1(3):159-165
The production of volatile organic compounds (VOCs) through the activation of different signal-transduction pathways may be
induced in various biotic and abiotic stress situations having importance e.g. in insect and disease resistance. We compared
the emission of VOCs emitted from silver birch Betula pendula Roth (clones 4 and 80) twigs damaged either by larvae of Epirrita autumnata, or infected with pathogenic leaf spot causing fungus Marssonina betulae. We also analysed whether local herbivore damage can systemically induce the release of VOCs from the undamaged top of same
sapling. The emissions of methylsalicylate (MeSA), (Z)-ocimene, (E)-β-ocimene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and linalool were induced from the twigs after 72 h feeding damage by E. autumnata larvae. However, 48 h feeding damage did not induce rapid systemic release of VOCs from undamaged top leaves of the same
twigs. Pathogen-infected birch twigs had significantly greater emission of (Z)-ocimene and (E)-β-ocimene than intact control twigs. The emission of DMNT was not significantly induced and MeSA was not found at all after
pathogen infection, both being significantly different from herbivore damaged twigs. According to our results leaf fungal
pathogen induces VOC emission profile differs from that of arthropod herbivore-damaged leaves, suggesting that birch is able
to transmit parasite-specific information via VOC emissions to conspecifics and natural enemies of herbivores.
Handling editor: Yvan Rahbé 相似文献
104.
Threat spots and environmental determinants of red-listed plant,butterfly and bird species in boreal agricultural environments 总被引:1,自引:0,他引:1
Sonja Kivinen Miska Luoto Risto K. Heikkinen Kimmo Saarinen Terhi Ryttäri 《Biodiversity and Conservation》2008,17(13):3289-3305
The aims of this study were (1) to examine the geographic distribution of red-listed species of agricultural environments
and identify their national threat spots (areas with high diversity of threatened species) in Finland and (2) to determine
the main environmental variables related to the richness and occurrence patterns of red-listed species. Atlas data of 21 plant,
17 butterfly and 11 bird species recorded using 10 km grid squares were employed in the study. Generalized additive models
(GAMs) were constructed separately for species richness and occurrence of individual species of the three species groups using
climate and land cover predictor variables. The predictive accuracy of models, as measured using correlation between the observed
and predicted values and AUC statistics, was generally good. Temperature-related variables were the most important determinants
of species richness and occurrence of all three taxa. In addition, land cover variables had a strong effect on the distribution
of species. Plants and butterflies were positively related to the cover of grasslands and birds to small-scale agricultural
mosaic as well as to arable land. Spatial coincidence of threat spots of plants, butterflies and birds was limited, which
emphasizes the importance of considering the potentially contrasting environmental requirements of different taxa in conservation
planning. Further, it is obvious that the maintenance of various non-crop habitats and heterogeneous agricultural landscapes
has an essential role in the preservation of red-listed species of boreal rural environments. 相似文献
105.
106.
Takeshi Inagawa Terhi Riutta Noreen Majalap-Lee Reuben Nilus James Josue Yadvinder Malhi 《Biotropica》2023,55(5):1019-1032
Nitrogen, phosphorus, potassium, calcium, and magnesium concentrations in woody tissue are poorly documented, but are necessary for understanding whole-tree nutrient use and storage. Here, we report how wood macronutrient concentrations vary radially and along the length of a tree for 10 tropical tree species in Sabah, Malaysia. Bark nutrient concentrations were consistently high: 2.9–13.7 times greater than heartwood depending on the nutrient. In contrast, within the wood both the radial (sapwood vs. heartwood) and vertical (trunk bottom vs. trunk middle) variation was modest. Higher concentrations in sapwood relative to heartwood provide empirical support for wood nutrient resorption during sapwood senescence. Dipterocarp species showed resorption rates of 25.3 ± 7.1% (nitrogen), 62.7 ± 11.9% (phosphorus), and 56.2 ± 12.5% (potassium), respectively, while non-dipterocarp species showed no evidence of nutrient resorption in wood. This suggests that while dipterocarps have lower wood nutrient concentrations, this family is able to compensate for this by using wood nutrient resorption as an efficient nutrient conservation mechanism. In contrast to other nutrients, calcium and magnesium tended to accumulate in heartwood. Wood density (WD) showed little vertical variation along the trunk. Across the species (WD range of 0.33 to 0.94 mg/cm3), WD was negatively correlated with wood P and K concentration and positively correlated with wood Ca concentration. As our study showed exceptionally high nutrient concentrations in the bark, debarking and leaving the bark of the harvested trees on site during logging operations could substantially contribute to maintaining nutrients within forest ecosystems. 相似文献
107.
Susanna Luoma Kirsi Peltoniemi Vesa Joutsjoki Terhi Rantanen Marja Tamminen Inka Heikkinen Airi Palva 《Applied microbiology》2001,67(3):1232-1238
For development of novel starter strains with improved proteolytic properties, the ability of Lactococcus lactis to produce Lactobacillus helveticus aminopeptidase N (PepN), aminopeptidase C (PepC), X-prolyl dipeptidyl aminopeptidase (PepX), proline iminopeptidase (PepI), prolinase (PepR), and dipeptidase (PepD) was studied by introducing the genes encoding these enzymes into L. lactis MG1363 and its derivatives. According to Northern analyses and enzyme activity measurements, the L. helveticus aminopeptidase genes pepN, pepC, and pepX are expressed under the control of their own promoters in L. lactis. The highest expression level, using a low-copy-number vector, was obtained with the L. helveticus pepN gene, which resulted in a 25-fold increase in PepN activity compared to that of wild-type L. lactis. The L. helveticus pepI gene, residing as a third gene in an operon in its host, was expressed in L. lactis under the control of the L. helveticus pepX promoter. The genetic background of the L. lactis derivatives tested did not affect the expression level of any of the L. helveticus peptidases studied. However, the growth medium used affected both the recombinant peptidase profiles in transformant strains and the resident peptidase activities. The levels of expression of the L. helveticus pepD and pepR clones under the control of their own promoters were below the detection limit in L. lactis. However, substantial amounts of recombinant pepD and PepR activities were obtained in L. lactis when pepD and pepR were expressed under the control of the inducible lactococcal nisA promoter at an optimized nisin concentration. 相似文献
108.
Increased Production of Xylanase by Expression of a Truncated Version of the xyn11A Gene from Nonomuraea flexuosa in Trichoderma reesei 下载免费PDF全文
Marja Paloheimo Arja Mntyl Jarno Kallio Terhi Puranen Pirkko Suominen 《Applied microbiology》2007,73(10):3215-3224
We have previously shown that the Nonomuraea flexuosa Xyn11A polypeptides devoid of the carbohydrate binding module (CBM) have better thermostability than the full-length xylanase and are effective in bleaching of pulp. To produce an enzyme preparation useful for industrial applications requiring high temperature, the region encoding the CBM was deleted from the N. flexuosa xyn11A gene and the truncated gene was expressed in Trichoderma reesei. The xylanase sequence was fused to the T. reesei mannanase I (Man5A) signal sequence or 3′ to a T. reesei carrier polypeptide, either the Man5A core/hinge or the cellulose binding domain (CBD) of cellobiohydrolase II (Cel6A, CBHII). The gene and fusion genes were expressed using the cellobiohydrolase 1 (cel7A, cbh1) promoter. Single-copy isogenic transformants in which the expression cassette replaced the cel7A gene were cultivated and analyzed. The transformants expressing the truncated N. flexuosa xyn11A produced clearly increased amounts of both the xylanase/fusion mRNA and xylanase activity compared to the corresponding strains expressing the full-length N. flexuosa xyn11A. The transformant expressing the cel6A CBD-truncated N. flexuosa xyn11A produced about 1.9 g liter−1 of the xylanase in laboratory-scale fermentations. The xylanase constituted about 25% of the secreted proteins. The production of the truncated xylanase did not induce the unfolded protein response (UPR) pathway. However, the UPR was induced when the full-length N. flexuosa xyn11A with an exact fusion to the cel7A terminator was expressed. We suggest that the T. reesei folding/secretion machinery is not able to cope properly with the bacterial CBM when the mRNA of the full-length N. flexuosa xyn11A is efficiently translated. 相似文献
109.
David Wilson Jukka Alm Terhi Riutta Jukka Laine Kenneth A. Byrne Edward P. Farrell Eeva-Stiina Tuittila 《Plant Ecology》2007,190(1):37-51
We studied vegetation dynamics at peatlands, differing in their climate, land use management history and vegetation community
in Ireland and Finland over a two-year period. Our aim was to develop a species-specific method to be used to (1) describe
the seasonal dynamics of green (photosynthetic) area (GA) of the vegetation and (2) incorporate these changes into CO2 exchange models. The extent of temporal and spatial variation between and within communities indicated the need for a two-step
calculation approach for each community. Firstly, at biweekly to monthly intervals, GA of all species within a range of vascular
plant communities was estimated by non-destructive field measurements. Gaussian or log-normal models were fitted to describe
the seasonal dynamics of each species. Secondly, an estimate of community vascular green area (VGA) was obtained by summing
the modelled daily GA of all species within the community. The highest values of VGA (2.1–6.0 m2 m−2) occurred within the reed communities at the rewetted cutaway peatland in Ireland and the lowest at the ombrotrophic lawn
communities in Finland (0.5–1.0 m2 m−2). The relationship between light saturated gross photosynthesis (P
G) and VGA was either linear or hyperbolic depending on the degree of self-shading that occurred within each community. The
addition of the VGA term into P
G models improved the explaining power of the model by 57.6, 24.5 and 23% within the Typha latifolia, Phalaris arundinacea and Eriophorum angustifolium/Carex rostrata communities, respectively. VGA proved useful in recording the seasonal development of a wide range of peatland vascular plant
communities over geographically and climatically different regions. 相似文献
110.
Tom Swinfield Sabine Both Terhi Riutta Boris Bongalov Dafydd Elias Noreen Majalap‐Lee Nicholas Ostle Martin Svtek Jakub Kvasnica David Milodowski Tommaso Jucker Robert M. Ewers Yi Zhang David Johnson Yit Arn Teh David F. R. P. Burslem Yadvinder Malhi David Coomes 《Global Change Biology》2020,26(2):989-1002
Logging, pervasive across the lowland tropics, affects millions of hectares of forest, yet its influence on nutrient cycling remains poorly understood. One hypothesis is that logging influences phosphorus (P) cycling, because this scarce nutrient is removed in extracted timber and eroded soil, leading to shifts in ecosystem functioning and community composition. However, testing this is challenging because P varies within landscapes as a function of geology, topography and climate. Superimposed upon these trends are compositional changes in logged forests, with species with more acquisitive traits, characterized by higher foliar P concentrations, more dominant. It is difficult to resolve these patterns using traditional field approaches alone. Here, we use airborne light detection and ranging‐guided hyperspectral imagery to map foliar nutrient (i.e. P, nitrogen [N]) concentrations, calibrated using field measured traits, over 400 km2 of northeastern Borneo, including a landscape‐level disturbance gradient spanning old‐growth to repeatedly logged forests. The maps reveal that canopy foliar P and N concentrations decrease with elevation. These relationships were not identified using traditional field measurements of leaf and soil nutrients. After controlling for topography, canopy foliar nutrient concentrations were lower in logged forest than in old‐growth areas, reflecting decreased nutrient availability. However, foliar nutrient concentrations and specific leaf area were greatest in relatively short patches in logged areas, reflecting a shift in composition to pioneer species with acquisitive traits. N:P ratio increased in logged forest, suggesting reduced soil P availability through disturbance. Through the first landscape scale assessment of how functional leaf traits change in response to logging, we find that differences from old‐growth forest become more pronounced as logged forests increase in stature over time, suggesting exacerbated phosphorus limitation as forests recover. 相似文献