Activated lymphocytes trigger lymphoblast extravasation.

Intraperitoneal stimulation of adoptively sensitized rats with bacterial antigen promotes the localization of lymphoblasts at the site of antigen deposition. Lymphoblast extravasation activity (LEA) is generated only when specifically immune donor lymphocytes and the recipients of these cells share at least on Ag-B haplotype. However, if the specificity criteria for its formation are satisfied, LEA promotes the local development of lymphoblasts of all available specificities and irrespective of their Ag-B genotype. Allogeneic lymphoblasts do not participate actively in the delayed inflammatory reaction even when they are passively recruited into exudates. The results suggest that LEA is a T cell-derived mediator that amplifies the delayed type hypersensitivity reaction by directing recently activated lymphocytes into lesions.     

In vivo bone strain on the dog tibia during locomotion

Rosette and single-element strain gauges were implanted on the tibia in 2 dogs and recordings were made during locomotion on a treadmill. At foot contact and during the swing phase of locomotion, bone strains were low and directions of the principal strains were variable. There was a large shift in the directions of the principal strains at the beginning of the stance phase and bone strains were considerably higher. Peak strain occurred midway through the stance phase. At that time, the maximum principal strain (tension) was directed upwards and anteriorly between 30 and 60 degrees with respect to the long axis of the tibia. These bone strain patterns in the dog are similar to those found in sheep while both differ markedly from those found in humans.     

Evaluation of the efficiency of measures for sulphidic mine waste mitigation

To control the environmentally detrimental impact of acid rock drainage, two different countermeasures, layers of acid-buffering materials and sodium dodecyl sulphate addition, were tested for their efficiency in laboratory percolation experiments. In the experiment with a layer of calcium bentonite, only the iron output was reduced. The experiments with layers of concrete grains demonstrated a decrease of the microbial activity as well as a precipitation of heavy-metal ions, whereas the cell numbers did not decrease. Furthermore, finely grained concrete (1–5 mm) formed a water-tight hardpan (self-sealing layer). In the experiment with 1 mM sodium dodecyl sulphate, all the microorganisms were killed and hence metal sulphide dissolution was stopped. With 0.1 mM sodium dodecyl sulphate only a short, transient inhibition of leaching was achieved. The bacteria remained alive. Received: 16 February 1998 / Accepted: 23 February 1998     

Locomotor behavior and skeletal morphology of two sympatric pitheciine monkeys,Pithecia pithecia and Chiropotes satanas

Field observations of two sympatric pitheciine species reveal that the positional repertoire of the white-faced saki, Pithecia pithecia, is dominated by leaping behaviors, whereas the bearded saki, Chiropotes satanas, is predominantly quadrupedal. Examination and comparison of the postcranial skeletal morphologies and limb proportions of these species display numerous features associated with their respective locomotor behaviors. These observations accord with associations found in other primate and mammalian groups and with predictions based on theoretical and experimental biomechanics. Preliminary observations of the skeletal morphology of Cacajao calvus demonstrate a marked similarity to that of Chiropotes. The fossil platyrrhine Cebupithecia sarmientoi displays greater similarity to Pithecia, suggesting that its positional repertoire also included significant leaping and clinging behaviors.     

IgG production kinetics in serum free media

Summary A murine hybridoma (455) was cultured in four different serum free media formulations, and a newborn calf serum supplemented medium was used as a basis of comparison. The serum supplemented medium supports a higher cell growth rate and results in a higher IgG titer. However, the antibody secretion rate on a per cell basis is higher in the serum free media, indicating that serum could be inhibitory to antibody secretion. The results identify the possibility of a least eliminating serum during the monoclonal antibody production phase.