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281.
The present study characterizes four potentially informative polymorphic bands of 5.2, 2.3, 1.9, and 1.2 kb, detected by Southern blot hybridization of Eco RI digests of human DNA using HK101/80 (an immunoglobulin V
I probe). These restriction fragment length polymorphisms (RFLP) show Mendelian segregation and they are linked to each other and to Km(1), the allotypic marker on the kappa constant region. There is strong linkage disequilibrium between the 2.3 and 1.2 kb polymorphisms. A 0.7 kb Pst I polymorphic band and a 2.9 kb Sac I polymorphic band were also identified; the latter may reflect a region of tandem repeats in the V
region. No bands representing the alternative forms of any of the polymorphic restriction sites were identified. This implies either that genes are missing from the V
repertoire or that such bands are hidden because of comigration of fragments due to conservation of restriction sites. Evidence for comigration of fragments was obtained from independent V
clones and suggests that dark bands on Southern blots of Pst I digests must often represent several superimposed genes which have conserved restriction sites. The demonstration of RFLP within the V region provides circumstantial evidence for polymorphic variation in the repertoire of V
structural genes. The RFLP reported here should be useful as genetic markers in future studies on the immune response and disease susceptibility in man. 相似文献
282.
Homozygous lymphoblastoid cell lines representing various Dw subtypes of DR2 were examined for polymorphism at the DQ
locus by molecular and cellular techniques. The subtypes studied included Dw2, Dw12, and a group heterogenous by cellular typing that we shall refer to as non-Dw2/non-Dw12. Restriction fragment length polymorphism analysis of cell lines representing these subtypes revealed DQ
-specific patterns consistent with cellular typing. Two-dimensional gel electrophoresis of DQ molecules from representative cell lines revealed a structural polymorphism of DQ
among the three subtypes. The DQ
chain migrated to a position that was unique to each subtype and was consistent among various representative cell lines of each subtype. Nucleotide sequence analysis of cDNA clones of DQ
from Dw2, Dw12, and non-Dw2/non-Dw12 lines confirmed that the variability resided at the genetic level. Variability was found in the form of numerous scattered nucleotide substitutions throughout the first domain of these alleles. The DQ
gene of the non-Dw2/non-Dw12 cell line AZH was further found to be almost identical with the DQ
gene of a DR1 line (Bell et al. 1985b), implicating a common evolutionary origin of these alleles. The only difference between these two sequences was due to an apparent gene conversion event at amino acid 57. T-cell cloning experiments resulted in the derivation of Epstein-Barr virus-specific, DQw1-restricted clones that proliferated against only those cell lines that exhibited the DQ
gene common to AZH and the DR1 cell line. Thus, the polymorphism among DQ
alleles within DR2 results in subtype-specific restriction. 相似文献
283.
John R. Lawrence Pascal J. Delaquis Darren R. Korber Douglas E. Caldwell 《Microbial ecology》1987,14(1):1-14
Phase, darkfield, and computer-enhanced microscopy were used to observe the surface microenvironment of flow cells during bacterial colonization. Microbial behavior was consistent with the assumptions used previously to derive surface colonization kinetics and to calculate surface growth and attachment rates from cell number and distribution. Surface microcolonies consisted of closely packed cells. Each colony contained 2n cells, where n is the number of cell divisions following attachment. Initially, cells were freely motile while attached, performing circular looping movements within the plane of the solid-liquid interface. Subsequently, cells attached apically, maintained a fixed position on the surface, and rotated. This type of attachment was reversible and did not necessarily lead to the formation of microcolonies. Cells became irreversibly attached by progressing from apical to longitudinal attachment. Longitudinally attached cells increased in length, then divided, separated, moved apart laterally, and slid next to one another. This resulted in tight cell packing and permitted simultaneous growth and adherence. After approximately 4 generations, individual cells emigrated from developing microcolonies to recolonize the surface at new locations. Surface colonization byPseudomonas fluorescens can thus be subdivided into the following sequential colonization phases: motile attachment phase, reversible attachment phase, irreversible attachment phase, growth phase, and recolonization phase. 相似文献
284.
The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels. 相似文献
285.
Carole L. Harker Philip M. Mullineaux John A. Bryant Andrew J. Maule 《Plant molecular biology》1987,8(3):275-287
Specific antisera were prepared to the inclusion body protein (gene VI product) and the gene I product of cauliflower mosaic virus (CaMV). Translational fusions between the lacZ gene and gene VI or gene I were constructed by cloning the relevant DNA fragments into the expression vectors pUR290, pUR291 or pUR292. Large amounts of fusion protein were synthesized when the inserted DNA fragment was in frame with the lacZ gene of the expression vector. These fusion proteins were used to raise specific antisera to gene VI and gene I proteins of CaMV. Antiserum to the gene VI product detected a range of proteins in crude extracts and in a subcellular fraction enriched for virus inclusion bodies. This range of proteins was further shown to be related to gene VI by Staphylococcus aureus V8 partial proteolysis. Antiserum to the gene I product detected viral specific proteins of 46, 42 and 38 K in preparations of CaMV replication complexes from infected plants but not in any other subcellular fraction. 相似文献
286.
Big Moose Basin: simulation of response to acidic deposition 总被引:2,自引:1,他引:1
George F. Davis John J. Whipple Steven A. Gherini Carl W. Chen Robert A. Goldstein Arland H. Johannes Peter W.H Chan Ronald K. Munson 《Biogeochemistry》1987,3(1-3):141-161
The ILWAS model has been enhanced for application to multiple-lake hydrologic basins. This version of the model has been applied to the Big Moose basin, which includes Big Moose Lake and its tributary streams, lakes, and watersheds. The basin, as defined, includes an area of 96 km2, with over 20 lakes and ponds, and 70 km of streams. Hydrologic and chemical calibrations have been made using data from seven sampling stations. When total atmospheric sulfur loading to the basin is halved, the model predicts, after four years of simulation, a decreasing sulfate concentration and to a lesser extent a rising alkalinity at Big Moose Lake outlet. At the end of four years, the results show an increase in pH of 0.1 to 0.5 pH units depending upon season. 相似文献
287.
Cost of tokking: the energetics of substrate communication in the tok-tok beetle,Psammodes striatus 总被引:1,自引:0,他引:1
John R. B. Lighton 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1987,157(1):11-20
Summary
Psammodes striatus, the tok-tok beetle, communicates by substrate vibrations produced by tapping its abdomen on the ground. MaleP. striatus responded readily to computer-synthesized vibrations. Oxygen consumption rate (
) of individual male beetles (n=3; mean mass 3.01 g) was continually recorded by computer before, during and after tapping communication with the computer, which also counted the beetle's replies. Standard
of motionless beetles was also measured, during which time marked discontinuous ventilation was apparent. Since the relation between
and tapping rate was linear, it was possible to estimate net cost of tapping (0.0279 l O2 g–1 tap–1) and minimum cost of tapping (0.0286 l O2 g–1 tap–1). Kinematic analysis of trains of taps showed constant intertap period (ca. 150 ms) and distinctive amplitude modulation. The efficiency of muscular movement inP. striatus is 23% of metabolic input, assuming no elastic storage. This figure will drop to ca. 5–10% if significant elastic storage takes place. Minimum cost of searching for a mate via pedestrian locomotion is 340 J kg–1 m–2, but drops to 33 J kg–1 m–2 for tapping communication. Similar energetic ratios may play a role in the early stages of the evolution of some communication systems.Abbreviation
STT
computer-synthetized tap train 相似文献
288.
John T. Hogg 《Ethology : formerly Zeitschrift fur Tierpsychologie》1987,75(2):119-144
Rocky Mountain bighorn (Ovis canadensis canadensis) rams employed three distinct mating tactics. When tending, rams defended single estrous ewes. In coursing, rams forced copulations with defended ewes, and, in blocking, rams sequestered ewes from more dominant rams. Ewes utilized a traditional area when tended, attempted to escape to this area when blocked, and resisted coursing ram attempts to force copulations. Between-year variation in the dispersion of estrous ewes about the tending area strongly influenced the consort and probably mating success of dominant rams. Thus, ewe spatial predictability during estrus — achieved by clustering in tended estrus and resisting blocking rams — appears to be an important mechanism of mate choice in this species. Ewes apparently did not gain material or risk-related benefits by mating dominant rams. That such males provide ewes with “good genes” is an attractive remaining possibility. 相似文献
289.
Abstract: A series of l-phenyl-1 H -3-benzazepine analogues were assessed for enantiomeric and structure-affinity relationships at human putamen D-1 dopamine receptors labelled with [3 H]SCH 23390. Substitution at the 7-position of both 3-H and 3-methyl benzazepine molecules critically affected affinity for these receptors over a 500-fold range. The general rank order of potency of 7-substituents was Cl = Br ≫ CH3 > OH ≥ H. 3-Methyl substituents increased the affinity of 7-H and 7-OH compounds two- to fivefold compared to desmethyl counterparts. The displacement of [3 H]SCH 23390 binding showed substantial enantioselec-tivity; the R-enantiomer of SKF 83566 was 500-fold more potent that its S-antipode. However, the displacement of [3 H]spiperone binding from D-2 sites in the same tissue showed negligible enantioselectivity. Through such structure-affinity relationships, these studies may help to define the topography of the human brain D-1 dopamine receptor and guide the design of more selecive agents for functional studies. 相似文献
290.
Molecular Forms of Acetylcholinesterase and Butyrylcholinesterase in Human Plasma and Cerebrospinal Fluid 总被引:1,自引:0,他引:1
John R. Atack Elaine K. Perry James R. Bonham Robert H. Perry 《Journal of neurochemistry》1987,48(6):1845-1850
The measurement of cholinesterase activities in either plasma or cerebrospinal fluid (CSF) may ultimately prove to be relevant in the diagnosis of neurological and neuropsychiatric disorders. However, studies to date have examined only total enzyme activities. Therefore in the present study we have examined the distribution of the individual molecular forms of both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in plasma and CSF using sucrose density gradient centrifugation. Although the total activities of AChE were of the same order of magnitude in plasma and CSF, there was a considerable difference (120-500-fold) between total BChE activity in the CSF and the BChE-rich plasma. The analysis of the individual molecular forms revealed that the predominant molecular species of AChE and BChE in the CSF--both lumbar and ventricular--was the G4 form. The G4 form also constituted the majority of the plasma BChE activity and, on average, over half (56%) of the plasma AChE activity. The significance of the AChE and BChE molecular form compositions of both plasma and CSF and their possible relationship to pathological states are discussed. 相似文献