全文获取类型
收费全文 | 167809篇 |
免费 | 6797篇 |
国内免费 | 846篇 |
专业分类
175452篇 |
出版年
2022年 | 486篇 |
2021年 | 942篇 |
2020年 | 567篇 |
2019年 | 721篇 |
2018年 | 12558篇 |
2017年 | 11345篇 |
2016年 | 8757篇 |
2015年 | 2962篇 |
2014年 | 2996篇 |
2013年 | 4023篇 |
2012年 | 8719篇 |
2011年 | 17269篇 |
2010年 | 14840篇 |
2009年 | 10878篇 |
2008年 | 13693篇 |
2007年 | 15324篇 |
2006年 | 4359篇 |
2005年 | 4569篇 |
2004年 | 5107篇 |
2003年 | 4754篇 |
2002年 | 4535篇 |
2001年 | 1122篇 |
2000年 | 764篇 |
1999年 | 913篇 |
1998年 | 1195篇 |
1997年 | 875篇 |
1996年 | 796篇 |
1995年 | 760篇 |
1994年 | 744篇 |
1993年 | 714篇 |
1992年 | 643篇 |
1991年 | 641篇 |
1990年 | 586篇 |
1989年 | 623篇 |
1988年 | 545篇 |
1987年 | 523篇 |
1986年 | 461篇 |
1985年 | 593篇 |
1984年 | 752篇 |
1983年 | 658篇 |
1982年 | 761篇 |
1981年 | 796篇 |
1980年 | 721篇 |
1979年 | 500篇 |
1978年 | 557篇 |
1977年 | 527篇 |
1976年 | 525篇 |
1974年 | 498篇 |
1972年 | 516篇 |
1971年 | 537篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
Evidence that the intestinal microbiota is intrinsically linked with overall health, including cancer risk, is emerging. Moreover, its composition is not fixed but can be influenced by several dietary components. Dietary modifiers, including the consumption of live bacteria (probiotics) and indigestible or limited digestible food constituents such as oligosaccharides (prebiotics) and polyphenols or both (synbiotics), are recognized modifiers of the numbers and types of microbes and have been reported to reduce colon cancer risk experimentally. Microorganisms also have the ability to generate bioactive compounds from food components. Examples include equol from isoflavones, enterodiol and enterolactone from lignans and urolithins from ellagic acid, which have also been demonstrated to retard experimentally induced cancers. The gastrointestinal microbiota can also influence both sides of the energy balance equation, namely, as a factor influencing energy utilization from the diet and as a factor that influences host genes that regulate energy expenditure and storage. Because of the link between obesity and cancer incidence and mortality, this complex complexion deserves greater attention. Overall, a dynamic interrelationship exists between the intestinal microbiota and colon cancer risk, which can be modified by dietary components and eating behaviors. 相似文献
32.
33.
Background
Spidroins are a unique family of large, structural proteins that make up the bulk of spider silk fibers. Due to the highly variable nature of their repetitive sequences, spidroin evolutionary relationships have principally been determined from their non-repetitive carboxy (C)-terminal domains, though they offer limited character data. The few known spidroin amino (N)-terminal domains have been difficult to obtain, but potentially contain critical phylogenetic information for reconstructing the diversification of spider silks. Here we used silk gland expression data (ESTs) from highly divergent species to evaluate the functional significance and phylogenetic utility of spidroin N-terminal domains. 相似文献34.
Potential efficacy of zileuton, a 5-LOX inhibitor, was evaluated for the reduction of pulmonary adenomas in the A/J murine
model when administered via nose-only inhalation. Development of pulmonary adenomas was induced with benzo(a)pyrene. Animals were treated with a zileuton solution (5 mg/mL in 85:15 ethanol/water) either twice weekly or five times
a week via nose-only inhalation; The placebo solution (85:15 EtOH/H2O, no active) was also evaluated. Dose delivered was calculated to be 1.2 mg/kg per exposure for each zileuton group. After
20 weeks of treatment, surface tumors were enumerated and histologically assessed. A significant reduction in tumor count
was noted for both the twice weekly administration (40%) and the five times a week administration (59%). The data also showed
a significant reduction for the group, which received the placebo (approximately 58%). The treatment groups were also found
to have an impact on the histological stages of adenoma development. 相似文献
35.
Mandana Nikpour Dafna D Gladman Dominique Ibanez Paula J Harvey Murray B Urowitz 《Arthritis research & therapy》2010,12(3):R125
Introduction
Total cholesterol (TC) and blood pressure (BP) are likely to take a dynamic course over time in patients with systemic lupus erythematosus (SLE). This would have important implications in terms of using single-point-in-time measurements of these variables to assess coronary artery disease (CAD) risk. The objective of this study was to describe and quantify variability over time of TC and BP among patients with SLE and to determine their correlates. 相似文献36.
Thorsten Thye Genevieve Scarisbrick Edmund N. L. Browne Margaret Amanua Chinbuah John Gyapong Ivy Osei Ellis Owusu-Dabo Stefan Niemann Sabine Rüsch-Gerdes Christian G. Meyer Rolf D. Horstmann 《PloS one》2009,4(7)
The gene of Cytotoxic T Lymphocyte-associated Antigen 4 (CTLA4), a negative regulator of T lymphocytes, contains a single-nucleotide polymorphism (SNP) at position +6230A->G (ct60A->G), which has been found associated with several autoimmune diseases and appears to reduce T-cell inhibitory activity. In Ghana, West Africa, we compared the frequencies of CTLA4 +6230 A/G and 6 haplotype-tagging SNPs in 2010 smear-positive, HIV-negative patients with pulmonary tuberculosis (TB) and 2346 controls matched for age, gender and ethnicity. We found no difference in allele frequencies between cases and controls. However, +6230A and a distinct CTLA4 haplotype and a diplotype comprising the +6230A allele were significantly less frequent among cases with large opacities in chest radiographs compared to those with small ones (Pcorrected [cor] = 0.002, Pcor = 0.00045, P = 0.0005, respectively). This finding suggests that an increased T-cell activity associated with the CTLA4 +6230G allele contributes to pathology rather than to protection in pulmonary TB. 相似文献
37.
38.
Kondeti Subramanyam K. V. Sailaja Koona Subramanyam D. Muralidhara Rao K. Lakshmidevi 《Plant Cell, Tissue and Organ Culture》2011,105(2):181-192
Plants, when exposed to abiotic or biotic stress, produce several pathogenesis-related proteins to counteract the effects
of stress. Osmotin is one of the important pathogenesis-related proteins induced during several stress conditions. We have
developed improved salt stress tolerant transgenic chilli pepper plants (Capsicum annum L. var. Aiswarya 2103) by ectopic expression of the Nicotiana tabaccum osmotin gene using Agrobacterium
tumefaciens EHA105 as a vector. Four-week-old chilli pepper leaves were used as an explant and A. tumefaciens EHA105 harboring pBINASCOSM plasmid that contains osmotin gene under the control of CaMV 35S promoter and npt II as a selectable marker was used in co-cultivation. Transgene integration and expression were analyzed using molecular,
immunochemical, and biochemical assays. PCR and Southern blot analysis confirmed that osmotin gene has been successfully integrated
into the genome of chilli pepper plants. The osmotin gene was stably segregated and expressed in T2 generation transgenic chilli pepper plants, and it was confirmed by Western blot analysis. Biochemical assays of these putative
transgenic plants revealed enhanced levels of chlorophyll, proline, glycinebetaine, APX, SOD, DHAR, MDHAR, GR, and relative
water content. Yield potential of the putative transgenic chilli pepper plants was evaluated under salinity stress conditions
in a green house. The putative transgenic chilli pepper plants overexpressing the osmotin gene were morphologically similar
to wild-type plants and produced 3.32 kg chilli pepper fruits per plant at 300 mM NaCl concentration. 相似文献
39.
Andrew Leask 《Journal of cell communication and signaling》2010,4(1):71-72
Connective tissue growth factor (CTGF/CCN2) is overexpressed in diabetes. Diabetic rats possess myocardial and cardiomyocyte
hypertrophy. In a recent report, Wang and colleagues (Am J Physiol Cell Physiol. 2009 Jul 22. [Epub ahead of print]) show
that CCN2 directly mediates cardiomyocyte hypertrophy as well as that induced by high glucose and fatty acid. CCN2 acted via
the TrkA receptor. These data are the subject of this commentary, and emphasize that CCN2 may be an excellent target for therapy
in diabetes. 相似文献
40.
The ability of an invasive plant to occupy new areas is often attributed to both morphological and physiological plasticities
that allow them to remain viable over a wide range of environmental conditions. Studies addressing the ecological requirements
of Microstegium vimineum often consider soil moisture or soil moisture along with other factors as important explanatory components for the establishment
and persistence of this invasive monocot. However, controlled studies specifically targeting water relations in M. vimineum are needed. Therefore, the purpose of this study was to determine how different water availabilities influence the growth
and physiological performance of M. vimineum. This study utilized experimental microcosms to achieve different water availabilities including low soil moisture (<15%
water), moderate soil moisture (ca. 20–30%), and flooded conditions. While both flooded and low soil moisture resulted in
diminished growth, M. vimineum still survived under these conditions. Physiological processes including C4 metabolism, minimum stress under low water conditions, and the ability to increase tissue rigidity may confer some advantages
to M. vimineum during periods of limiting water conditions. Similarly, the proportionally low root biomass, shallow root structure, and
its ability to maintain stable water relations during flooding and/or soil waterlogging may facilitate M. vimineum’s ability to invade mesic habitats. It is likely, therefore, that the capacity to tolerate both low soil moistures and flooded
conditions has enhanced the ability of M. vimineum to populate disturbed systems in central North Carolina. 相似文献