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111.
Atsuko Satoh Jong-Keun Kim Ikuko Miyahara Bart Devreese Isabel Vandenberghe Ayse Hacisalihoglu Toshihide Okajima Shun'ichi Kuroda Osao Adachi Johannis A Duine Jozef Van Beeumen Katsuyuki Tanizawa Ken Hirotsu 《The Journal of biological chemistry》2002,277(4):2830-2834
The crystal structure of a quinohemoprotein amine dehydrogenase from Pseudomonas putida has been determined at 1.9-A resolution. The enzyme comprises three non-identical subunits: a four-domain alpha-subunit that harbors a di-heme cytochrome c, a seven-bladed beta-propeller beta-subunit that provides part of the active site, and a small gamma-subunit that contains a novel cross-linked, proteinous quinone cofactor, cysteine tryptophylquinone. More surprisingly, the catalytic gamma-subunit contains three additional chemical cross-links that encage the cysteine tryptophylquinone cofactor, involving a cysteine side chain bridged to either an Asp or Glu residue all in a hitherto unknown thioether bonding with a methylene carbon atom of acidic amino acid side chains. Thus, the structure of the 79-residue gamma-subunit is quite unusual, containing four internal cross-links in such a short polypeptide chain that would otherwise be difficult to fold into a globular structure. 相似文献
112.
Johannis P. Kamerling Josef Makovitzky Roland Schauer Johannes F.G. Vliegenthart Margret Wember 《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,714(2):351-355
Analysis of the sialic acids obtained by mild acid hydrolysis of B lymphocytes reveals the presence of N-acetylneuraminic acid and 9-O-acetyl-N-acetylneuraminic acid. For T lymphocytes only N-acetylneuraminic acid has been demonstrated to occur. The applied methods include quantitative colorimetry, thin-layer chromatography and combined gas-liquid chromatography-mass spectrometry. 相似文献
113.
Glycoconjugate Journal - 相似文献
114.
Herman Van Halbeek Johan Haverkamp Johannis P. Kamerling Johannes F.G. Vliegenthart Cornelis Versluis Roland Schauer 《Carbohydrate research》1978,60(1):51-62
A set of partially O-methylated derivatives of methyl N- acetyl-N-methyl-β-D-neuraminate methyl glycoside has been prepared as reference compounds for the Incorporation of acylneuraminic acids into methylation analysis. G.1.c.-m.s. data for the O-trimethylsilyl and O-acetyl derivatives of these compounds are described in detail. The various substances give rise to highly characteristic mass-spectrometric fragmentation-patterns. 相似文献
115.
116.
Kanika Vanshylla Veronica Di Cristanziano Franziska Kleipass Felix Dewald Philipp Schommers Lutz Gieselmann Henning Gruell Maike Schlotz Meryem S. Ercanoglu Ricarda Stumpf Petra Mayer Matthias Zehner Eva Heger Wibke Johannis Carola Horn Isabelle Suárez Norma Jung Susanne Salomon Florian Klein 《Cell host & microbe》2021,29(6):917-929.e4
117.
Erika Staudacher Friedrich Altmann Leopold März Karl Hård Johannis P. Kamerling Johannes F. G. Vliegenthart 《Glycoconjugate journal》1992,9(2):82-85
Chymotryptic glycopeptides were prepared from a honeybee (Apis mellifica) venom phospholipase A2 (E.C. 3.1.1.4) fraction, with high affinity towards lentil (Lens culinaris) lectin. Treatment of the glycopeptide mixture with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A, followed by HPLC fractionation, yielded two oligosaccharides, which were analysed by 500 MHz 1H-NMR spectroscopy to give the following structures [formula: see text] This is the first report on a naturally occurring glycoprotein N-glycan with two fucose residues linked to the asparagine-bound N-acetylglucosamine. 相似文献
118.
Abstract The methylotrophic yeasts, Hansenula polymorpha and Candida boidinii , and the methylotrophic Gram-negative bacteria, Paracoccus denitrificans and Thiobacillus versutus (but not Methylophaga marina ), contain NAD/GSH-dependent formaldehyde dehydrogenase when grown on C1 -compounds. The enzymes appeared to be similar to each other and to the mammalian counterparts with respect to substrate specificity, including the ability to act as an alcohol dehydrogenase class III. The Gram-positive bacteria, Amycolatopsis methanolica and Rhodococcus erythropolis , possess NAD/Factor-dependent formaldehyde dehydrogenase when grown on C1 -compounds or on C1 -unit-containing substrates, respectively. These enzymes also exhibit alcohol dehydrogenase class III activity. Thus, like the mammalian ones, methylotrophic formaldehyde dehydrogenases show dual substrate specificity, suggesting that this is an inherent property of the enzyme. 相似文献
119.
Andrea van der Meer Johannis P. Kamerling Johannes F.G. Vliegenthart Karl Schmid Roland Schauer 《Biochimica et Biophysica Acta (BBA)/General Subjects》1983,757(3):371-376
Analysis of the sialic acid fraction obtained by mild acid hydrolysis of the Cuvierian tubules of the sea cucumber Holothuria forskali Della Chiaje revealed the presence of F-glycolylneuraminic acid and fucopyranosyl-(1 → 4)-N-glycolylneuraminic acid. Furthermore, methylation analysis of a pronase-digest of the tubules demonstrated that all N-glycolylneuraminic acid residues are substituted at C-4, explaining the earlier reported resistance of sialic acid in tubules to cleavage by neuraminidase. 相似文献
120.
Johannis P. Kamerling Gérard Strecker Jean-Pierre Farriaux Lambertus Dorland Johan Haverkamp Johannes F.G. Vliegenthart 《Biochimica et Biophysica Acta (BBA)/General Subjects》1979,583(3):403-408
A new metabolite, namely 2-acetamidoglucal, has been found in the urine of a patient with sialuria in addition to the metabolites N-acetylneuraminic acid, N-acetylmannosamine, N-acetylglucosamine and N-deoxy-2,3-dehydro-Nacetylneuraminic acid reported earlier. The structure has been identified by mass spectrometry and 360 MHz proton nuclear magnetic resonance spectroscopy and verified by synthesis. All accumulated compounds fit into the metabolic pathway for the biosynthesis of CMP-N-acetylneuraminic acid. Sialuria is discussed in terms of a failure of regulation of UDP-N-acetyl-glucosamine 2-epimerase. 相似文献