全文获取类型
收费全文 | 4253篇 |
免费 | 415篇 |
出版年
2022年 | 37篇 |
2021年 | 78篇 |
2020年 | 40篇 |
2019年 | 50篇 |
2018年 | 58篇 |
2017年 | 59篇 |
2016年 | 102篇 |
2015年 | 193篇 |
2014年 | 202篇 |
2013年 | 224篇 |
2012年 | 308篇 |
2011年 | 267篇 |
2010年 | 161篇 |
2009年 | 140篇 |
2008年 | 214篇 |
2007年 | 213篇 |
2006年 | 176篇 |
2005年 | 195篇 |
2004年 | 180篇 |
2003年 | 170篇 |
2002年 | 127篇 |
2001年 | 65篇 |
2000年 | 82篇 |
1999年 | 82篇 |
1998年 | 48篇 |
1997年 | 36篇 |
1996年 | 38篇 |
1995年 | 34篇 |
1994年 | 30篇 |
1993年 | 31篇 |
1992年 | 58篇 |
1991年 | 60篇 |
1990年 | 61篇 |
1989年 | 42篇 |
1988年 | 54篇 |
1987年 | 35篇 |
1986年 | 43篇 |
1985年 | 38篇 |
1984年 | 46篇 |
1983年 | 31篇 |
1982年 | 28篇 |
1979年 | 32篇 |
1977年 | 24篇 |
1976年 | 25篇 |
1975年 | 31篇 |
1974年 | 24篇 |
1973年 | 27篇 |
1971年 | 22篇 |
1969年 | 30篇 |
1966年 | 20篇 |
排序方式: 共有4668条查询结果,搜索用时 203 毫秒
51.
52.
1. Cellulose decomposition in forest and orchard soils was investigated by studying the breakdown of boiled and washed cellophane in the soils and in vitro. Decomposition occurred from quick to slow in the order: orchard on clay soil, forest on clay soil, forest on sandy loam, and in the latter in the order: calcareous mull, acid mull and mor. 2. In the different forest soils which were investigated the rate of decomposition was parallel to their water capacity. It slowed down considerably when the water content of the soil decreased, especially after the wilting point was reached. 3. Of the fungi isolated from these soils, those from orchard soil — 5% to 50%Fusarium spp. — were among the fastest decomposers of cellulose. This agrees with, and may explain the high rate of decomposition in orchard soil. 4. Decomposition in pure culture is quicker than in soil. As filtersterilized soil extract checked the decomposition in pure culture, but heat-sterilized soil extract did not, an extractable but heat-sensitive substance may be one retarding factor. 相似文献
53.
54.
55.
56.
57.
58.
Marc Yudkoff David Pleasure Lynn Cregar Zhi-Ping Lin Ilana Nissim Janet Stern Itzhak Nissim 《Journal of neurochemistry》1990,55(1):137-145
The incorporation of [15N]glutamic acid into glutathione was studied in primary cultures of astrocytes. Turnover of the intracellular glutathione pool was rapid, attaining a steady state value of 30.0 atom% excess in 180 min. The intracellular glutathione concentration was high (20-40 nmol/mg protein) and the tripeptide was released rapidly into the incubation medium. Although labeling of glutathione (atom% excess) with [15N]glutamate occurred rapidly, little accumulation of 15N in glutathione was noted during the incubation compared with 15N in aspartate, glutamine, and alanine. Glutathione turnover was stimulated by incubating the astrocytes with diethylmaleate, an electrophile that caused a partial depletion of the glutathione pool(s). Diethylmaleate treatment also was associated with significant reductions of intraastrocytic glutamate, glycine, and cysteine, i.e., the constituents of glutathione. Glutathione synthesis could be stimulated by supplementing the steady-state incubation medium with 0.05 mM L-cysteine, such treatment again partially depleting intraastrocytic glutamate and causing significant reductions of 15N labeling of both alanine and glutamine, suggesting that glutamate had been diverted from the synthesis of these amino acids and toward the formation of glutathione. The current study underscores both the intensity of glutathione turnover in astrocytes and the relationship of this turnover to the metabolism of glutamate and other amino acids. 相似文献
59.
To study their role in the structure and function of bacteriorhodopsin, three prolines, presumed to be in the membrane-embedded alpha-helices, have been individually replaced as follows: Pro-50 and Pro-91 each by Gly and Ala and Pro-186 by Ala, Gly, and Val. The mutants of Pro-50 and Pro-91 all showed normal chromophore and proton pumping. However, the rates of regeneration of the chromophore in Pro-50----Ala, Pro-91----Ala and ----Gly with all-trans-retinal were about 30-fold slower than that in the wild-type, whereas the chromophore regeneration rate in Pro-50----Gly was 10-fold faster than in the wild-type. While, Pro-186----Ala regenerated the wild-type chromophore, the mutants Pro-186----Val and Pro-186----Gly showed large blue shifts (about 80 nm) in the chromophore regenerated with all-trans-retinal and showed no apparent dark-light adaptation. Pro-186----Gly first regenerated the wild-type chromophore with 13-cis-retinal which was thermally unstable and rapidly converted to the blue-shifted chromophore obtained with all-trans-retinal. High salt concentration restored the wild-type purple chromophore in the Pro-186----Gly mutant. Thus, in this mutant, the protein interconverts between two conformational states. Pro-186----Ala and Pro-186----Gly showed about 65%, whereas Pro-186----Val showed 10-20% of the normal proton pumping. 相似文献
60.
Function of plastid mRNA 3' inverted repeats. RNA stabilization and gene-specific protein binding 总被引:33,自引:0,他引:33
Plastid protein coding regions in plants are generally flanked by 3' inverted repeat (IR) sequences. In a previous work (Stern, D. B., and Gruissem, W. (1987) Cell 51, 1145-1157), we have shown that their role may be in RNA stabilization and as a processing signal that establishes the mature mRNA 3' end. In this report we have investigated the stability and protein interaction of chloroplast mRNA 3' IR-RNA sequences in more detail. Progressive deletions into the 3' IR-RNA sequences for the chloroplast cytochrome b6/f subunit IV (petD) mRNA reduce the stability of the RNA, indicating that the potential to form a stem/loop is a minimum requirement for petD 3' IR-RNA stability in vitro. Specific point mutants also destabilize the processed 3' IR-RNA, suggesting an important role for the primary sequence. Gel mobility shift and UV-cross-linking analysis has shown that 3' IR-RNAs of petD and two other chloroplast mRNAs (rbcL and psbA) interact with proteins in vitro. Comparison of the bound petD 3' IR-RNA proteins with proteins that bind to rbcL and psbA reveals that binding of certain proteins is gene-specific. Also, precursor and processed petD 3' IR-RNAs bind different sets of proteins. A single nucleotide transversion (T----A) near the base of the stem eliminates the binding of a 29-kDa protein to the petD 3' IR-RNA precursor. We discuss the possible role of 3' IR-RNA-protein interactions in plastid mRNA 3' end maturation and differential mRNA stability. 相似文献