全文获取类型
收费全文 | 2821篇 |
免费 | 206篇 |
出版年
2023年 | 17篇 |
2022年 | 31篇 |
2021年 | 70篇 |
2020年 | 34篇 |
2019年 | 44篇 |
2018年 | 47篇 |
2017年 | 48篇 |
2016年 | 100篇 |
2015年 | 177篇 |
2014年 | 171篇 |
2013年 | 189篇 |
2012年 | 271篇 |
2011年 | 218篇 |
2010年 | 136篇 |
2009年 | 121篇 |
2008年 | 165篇 |
2007年 | 161篇 |
2006年 | 129篇 |
2005年 | 149篇 |
2004年 | 140篇 |
2003年 | 117篇 |
2002年 | 79篇 |
2001年 | 19篇 |
2000年 | 24篇 |
1999年 | 32篇 |
1998年 | 22篇 |
1997年 | 17篇 |
1996年 | 23篇 |
1995年 | 17篇 |
1994年 | 8篇 |
1993年 | 17篇 |
1992年 | 17篇 |
1991年 | 15篇 |
1990年 | 19篇 |
1989年 | 10篇 |
1988年 | 20篇 |
1987年 | 11篇 |
1986年 | 12篇 |
1985年 | 11篇 |
1984年 | 10篇 |
1983年 | 10篇 |
1982年 | 4篇 |
1981年 | 9篇 |
1980年 | 5篇 |
1979年 | 13篇 |
1976年 | 10篇 |
1975年 | 8篇 |
1972年 | 5篇 |
1966年 | 5篇 |
1931年 | 3篇 |
排序方式: 共有3027条查询结果,搜索用时 140 毫秒
211.
Daniel Berry Johanna E. Takach Christopher L. Schardl Nikki D. Charlton Barry Scott Carolyn A. Young 《Applied and environmental microbiology》2015,81(8):2797-2807
Peramine is an insect-feeding deterrent produced by Epichloë species in symbiotic association with C3 grasses. The perA gene responsible for peramine synthesis encodes a two-module nonribosomal peptide synthetase. Alleles of perA are found in most Epichloë species; however, peramine is not produced by many perA-containing Epichloë isolates. The genetic basis of these peramine-negative chemotypes is often unknown. Using PCR and DNA sequencing, we analyzed the perA genes from 72 Epichloë isolates and identified causative mutations of perA null alleles. We found nonfunctional perA-ΔR* alleles, which contain a transposon-associated deletion of the perA region encoding the C-terminal reductase domain, are widespread within the Epichloë genus and represent a prevalent mutation found in nonhybrid species. Disparate phylogenies of adjacent A2 and T2 domains indicated that the deletion of the reductase domain (R*) likely occurred once and early in the evolution of the genus, and subsequently there have been several recombinations between those domains. A number of novel point, deletion, and insertion mutations responsible for abolishing peramine production in full-length perA alleles were also identified. The regions encoding the first and second adenylation domains (A1 and A2, respectively) were common sites for such mutations. Using this information, a method was developed to predict peramine chemotypes by combining PCR product size polymorphism analysis with sequencing of the perA adenylation domains. 相似文献
212.
213.
Jenni Hultman Riitta Rahkila Javeria Ali Juho Rousu K. Johanna Bj?rkroth 《Applied and environmental microbiology》2015,81(20):7088-7097
Refrigerated food processing facilities are specific man-made niches likely to harbor cold-tolerant bacteria. To characterize this type of microbiota and study the link between processing plant and product microbiomes, we followed and compared microbiota associated with the raw materials and processing stages of a vacuum-packaged, cooked sausage product affected by a prolonged quality fluctuation with occasional spoilage manifestations during shelf life. A total of 195 samples were subjected to culturing and amplicon sequence analyses. Abundant mesophilic psychrotrophs were detected within the microbiomes throughout the different compartments of the production plant environment. However, each of the main genera of food safety and quality interest, e.g., Leuconostoc, Brochothrix, and Yersinia, had their own characteristic patterns of contamination. Bacteria from the genus Leuconostoc, commonly causing spoilage of cold-stored, modified-atmosphere-packaged foods, were detected in high abundance (up to >98%) in the sausages studied. The same operational taxonomic units (OTUs) were, however, detected in lower abundances in raw meat and emulsion (average relative abundance of 2% ± 5%), as well as on the processing plant surfaces (<4%). A completely different abundance profile was found for OTUs phylogenetically close to the species Yersinia pseudotuberculosis. These OTUs were detected in high abundance (up to 28%) on the processing plant surfaces but to a lesser extent (<1%) in raw meat, sausage emulsion, and sausages. The fact that Yersinia-like OTUs were found on the surfaces of a high-hygiene packaging compartment raises food safety concerns related to their resilient existence on surfaces. 相似文献
214.
Construction of a robust and sensitive arginine biosensor through ancestral protein reconstruction
下载免费PDF全文
![点击此处可从《Protein science : a publication of the Protein Society》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jason H Whitfield William H Zhang Michel K Herde Ben E Clifton Johanna Radziejewski Harald Janovjak Christian Henneberger Colin J Jackson 《Protein science : a publication of the Protein Society》2015,24(9):1412-1422
Biosensors for signaling molecules allow the study of physiological processes by bringing together the fields of protein engineering, fluorescence imaging, and cell biology. Construction of genetically encoded biosensors generally relies on the availability of a binding “core” that is both specific and stable, which can then be combined with fluorescent molecules to create a sensor. However, binding proteins with the desired properties are often not available in nature and substantial improvement to sensors can be required, particularly with regard to their durability. Ancestral protein reconstruction is a powerful protein-engineering tool able to generate highly stable and functional proteins. In this work, we sought to establish the utility of ancestral protein reconstruction to biosensor development, beginning with the construction of an l-arginine biosensor. l-arginine, as the immediate precursor to nitric oxide, is an important molecule in many physiological contexts including brain function. Using a combination of ancestral reconstruction and circular permutation, we constructed a Förster resonance energy transfer (FRET) biosensor for l-arginine (cpFLIPR). cpFLIPR displays high sensitivity and specificity, with a Kd of ∼14 µM and a maximal dynamic range of 35%. Importantly, cpFLIPR was highly robust, enabling accurate l-arginine measurement at physiological temperatures. We established that cpFLIPR is compatible with two-photon excitation fluorescence microscopy and report l-arginine concentrations in brain tissue. 相似文献
215.
Evidence of land‐sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species
下载免费PDF全文
![点击此处可从《Molecular ecology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Johanna L. Baily Guillaume Méric Sion Bayliss Geoffrey Foster Simon E. Moss Eleanor Watson Ben Pascoe Jane Mikhail Romain Pizzi Robert J. Goldstone David G. E. Smith Kim Willoughby Ailsa J. Hall Mark P. Dagleish 《Molecular ecology》2015,24(1):208-221
Environmental pollution often accompanies the expansion and urbanization of human populations where sewage and wastewaters commonly have an impact on the marine environments. Here, we explored the potential for faecal bacterial pathogens, of anthropic origin, to spread to marine wildlife in coastal areas. The common zoonotic bacterium Campylobacter was isolated from grey seals (Halichoerus grypus), an important sentinel species for environmental pollution, and compared to isolates from wild birds, agricultural sources and clinical samples to characterize possible transmission routes. Campylobacter jejuni was present in half of all grey seal pups sampled (24/50 dead and 46/90 live pups) in the breeding colony on the Isle of May (Scotland), where it was frequently associated with histological evidence of disease. Returning yearling animals (19/19) were negative for C. jejuni suggesting clearance of infection while away from the localized colony infection source. The genomes of 90 isolates from seals were sequenced and characterized using a whole‐genome multilocus sequence typing (MLST) approach and compared to 192 published genomes from multiple sources using population genetic approaches and a probabilistic genetic attribution model to infer the source of infection from MLST data. The strong genotype‐host association has enabled the application of source attribution models in epidemiological studies of human campylobacteriosis, and here assignment analyses consistently grouped seal isolates with those from human clinical samples. These findings are consistent with either a common infection source or direct transmission of human campylobacter to grey seals, raising concerns about the spread of human pathogens to wildlife marine sentinel species in coastal areas. 相似文献
216.
Johanna Chemnitz Petra C. Jentschke Manfred Ayasse Sandra Steiger 《Proceedings. Biological sciences / The Royal Society》2015,282(1812)
Long-range sex pheromones have been subjected to substantial research with a particular focus on their biosynthesis, peripheral perception, central processing and the resulting orientation behaviour of perceivers. Fundamental to the research on sex attractants was the assumption that they primarily coordinate species recognition. However, especially when they are produced by the less limiting sex (usually males), the evolution of heightened condition dependence might be expected and long-range sex pheromones might, therefore, also inform about a signaller''s quality. Here we provide, to our knowledge, the first comprehensive study of the role of a male''s long-range pheromone in mate choice that combines chemical analyses, video observations and field experiments with a multifactorial manipulation of males'' condition. We show that the emission of the long-distance sex pheromone of the burying beetle, Nicrophorus vespilloides is highly condition-dependent and reliably reflects nutritional state, age, body size and parasite load—key components of an individual''s somatic state. Both, the quantity and ratio of the pheromone components were affected but the time invested in pheromone emission was largely unaffected by a male''s condition. Moreover, the variation in pheromone emission caused by the variation in condition had a strong effect on the attractiveness of males in the field, with males in better nutritional condition, of older age, larger body size and bearing less parasites being more attractive. That a single pheromone is influenced by so many aspects of the somatic state and causes such variation in a male''s attractiveness under field conditions was hitherto unknown and highlights the need to integrate indicator models of sexual selection into pheromone research. 相似文献
217.
Christoph Gerhardt Johanna Maria Lier Stephan Burmühl Andreas Struchtrup Kathleen Deutschmann Maik Vetter Tristan Leu Sandra Reeg Tilman Grune Ulrich Rüther 《The Journal of cell biology》2015,210(1):1027-1045
Mutations in RPGRIP1L result in severe human diseases called ciliopathies. To unravel the molecular function of RPGRIP1L, we analyzed Rpgrip1l−/− mouse embryos, which display a ciliopathy phenotype and die, at the latest, around birth. In these embryos, cilia-mediated signaling was severely disturbed. Defects in Shh signaling suggested that the Rpgrip1l deficiency causes an impairment of protein degradation and protein processing. Indeed, we detected a cilia-dependent decreased proteasomal activity in the absence of Rpgrip1l. We found different proteasomal components localized to cilia and identified Psmd2, a component of the regulatory proteasomal 19S subunit, as an interaction partner for Rpgrip1l. Quantifications of proteasomal substrates demonstrated that Rpgrip1l regulates proteasomal activity specifically at the basal body. Our study suggests that Rpgrip1l controls ciliary signaling by regulating the activity of the ciliary proteasome via Psmd2. 相似文献
218.
Johanna Apro Paolo Parini Anders Broijersén Bo Angelin Mats Rudling 《Journal of lipid research》2015,56(8):1633-1639
At a given level of serum cholesterol, patients with T2D have an increased risk of developing atherosclerosis compared with nondiabetic subjects. We hypothesized that T2D patients have an increased interstitial fluid (IF)-to-serum gradient ratio for LDL, due to leakage over the vascular wall. Therefore, lipoprotein profiles in serum and IF from 35 T2D patients and 35 healthy controls were assayed using fast performance liquid chromatography. The IF-to-serum gradients for VLDL and LDL cholesterol, as well as for apoB, were clearly reduced in T2D patients compared with healthy controls. No such differences were observed for HDL cholesterol. Contrary to our hypothesis, the atherogenic VLDL and LDL particles were not increased in IF from diabetic patients. Instead, they were relatively sparser than in healthy controls. The most probable explanation to our unexpected finding is that these lipoproteins are more susceptible to retainment in the extravascular space of these patients, reflecting a more active uptake by, or adhesion to, tissue cells, including macrophages in the vascular wall. Further studies are warranted to further characterize the mechanisms underlying these observations, which may be highly relevant for the understanding of why the propensity to develop atherosclerosis is increased in T2D. 相似文献
219.
Johanna Ceschin Hans Caspar Hürlimann Christelle Saint-Marc Delphine Albrecht Typhaine Violo Michel Moenner Bertrand Daignan-Fornier Beno?t Pinson 《The Journal of biological chemistry》2015,290(39):23947-23959
5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside monophosphate (AICAR) is a natural metabolite with potent anti-proliferative and low energy mimetic properties. At high concentration, AICAR is toxic for yeast and mammalian cells, but the molecular basis of this toxicity is poorly understood. Here, we report the identification of yeast purine salvage pathway mutants that are synthetically lethal with AICAR accumulation. Genetic suppression revealed that this synthetic lethality is in part due to low expression of adenine phosphoribosyl transferase under high AICAR conditions. In addition, metabolite profiling points to the AICAR/NTP balance as crucial for optimal utilization of glucose as a carbon source. Indeed, we found that AICAR toxicity in yeast and human cells is alleviated when glucose is replaced by an alternative carbon source. Together, our metabolic analyses unveil the AICAR/NTP balance as a major factor of AICAR antiproliferative effects. 相似文献
220.
Johanna A. Bac-Molenaar Emilie F. Fradin Frank F.M. Becker Juriaan A. Rienstra J. van der Schoot Dick Vreugdenhil Joost J.B. Keurentjes 《The Plant cell》2015,27(7):1857-1874
For crops that are grown for their fruits or seeds, elevated temperatures that occur during flowering and seed or fruit set have a stronger effect on yield than high temperatures during the vegetative stage. Even short-term exposure to heat can have a large impact on yield. In this study, we used Arabidopsis thaliana to study the effect of short-term heat exposure on flower and seed development. The impact of a single hot day (35°C) was determined in more than 250 natural accessions by measuring the lengths of the siliques along the main inflorescence. Two sensitive developmental stages were identified, one before anthesis, during male and female meiosis, and one after anthesis, during fertilization and early embryo development. In addition, we observed a correlation between flowering time and heat tolerance. Genome-wide association mapping revealed four quantitative trait loci (QTLs) strongly associated with the heat response. These QTLs were developmental stage specific, as different QTLs were detected before and after anthesis. For a number of QTLs, T-DNA insertion knockout lines could validate assigned candidate genes. Our findings show that the regulation of complex traits can be highly dependent on the developmental timing. 相似文献