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Henrik Kibak Lincoln Taiz Thomas Starke Paul Bernasconi Johann Peter Gogarten 《Journal of bioenergetics and biomembranes》1992,24(4):415-424
Proton pumping ATPases/ATPsynthases are found in all groups of present-day organisms. The structure of V- and F-type ATPases/ATP synthases is very conserved throughout evolution. Sequence analysis shows that the V- and F-type ATPases evolved from the same enzyme already present in the last common ancestor of all known extant life forms. The catalytic and noncatalytic subunits found in the dissociable head groups of the V/F-type ATPases are paralogous subunits, i.e., these two types of subunits evolved from a common ancestral gene. The gene duplication giving rise to these two genes (i.e., encoding the catalytic and noncatalytic subunits) predates the time of the last common ancestor.Mapping of gene duplication events that occurred in the evolution of the proteolipid, the noncatalytic and the catalytic subunits, onto the tree of life leads to a prediction for the likely subunit structure of the encoded ATPases. A correlation between structure and function of V/F-ATPases has been established for present-day organisms. Implications resulting from this correlation for the bioenergetics operative in proto-eukaryotes and in the last common ancestor are presented. The similarities of the V/F-ATPase subunits to an ATPase-like protein that was implicated to play a role in flagellar assembly are evaluated.Different V-ATPase isoforms have been detected in some higher eukaryotes. These data are analyzed with respect to the possible function of the different isoforms (tissue specific, organelle specific) and with respect to the point in their evolution when these gene duplications giving rise to the isoforms had occurred, i.e., how far these isoforms are distributed. 相似文献
13.
Proton pumping ATPases are found in all groups of present day organisms. The F-ATPases of eubacteria, mitochondria and chloroplasts also function as ATP synthases, i.e., they catalyze the final step that transforms the energy available from reduction/oxidation reactions (e.g., in photosynthesis) into ATP, the usual energy currency of modern cells. The primary structure of these ATPases/ATP synthases was found to be much more conserved between different groups of bacteria than other parts of the photosynthetic machinery, e.g., reaction center proteins and redox carrier complexes.These F-ATPases and the vacuolar type ATPase, which is found on many of the endomembranes of eukaryotic cells, were shown to be homologous to each other; i.e., these two groups of ATPases evolved from the same enzyme present in the common ancestor. (The term eubacteria is used here to denote the phylogenetic group containing all bacteria except the archaebacteria.) Sequences obtained for the plasmamembrane ATPase of various archaebacteria revealed that this ATPase is much more similar to the eukaryotic than to the eubacterial counterpart. The eukaryotic cell of higher organisms evolved from a symbiosis between eubacteria (that evolved into mitochondria and chloroplasts) and a host organism. Using the vacuolar type ATPase as a molecular marker for the cytoplasmic component of the eukaryotic cell reveals that this host organism was a close relative of the archaebacteria.A unique feature of the evolution of the ATPases is the presence of a non-catalytic subunit that is paralogous to the catalytic subunit, i.e., the two types of subunits evolved from a common ancestral gene. Since the gene duplication that gave rise to these two types of subunits had already occurred in the last common ancestor of all living organisms, this non-catalytic subunit can be used to root the tree of life by means of an outgroup; that is, the location of the last common ancestor of the major domains of living organisms (archaebacteria, eubacteria and eukaryotes) can be located in the tree of life without assuming constant or equal rates of change in the different branches.A correlation between structure and function of ATPases has been established for present day organisms. Implications resulting from this correlation for biochemical pathways, especially photosynthesis, that were operative in the last common ancestor and preceding life forms are discussed. 相似文献
14.
An age-specific life table for the chicken miteDermanyssus gallinae (DeGeer, 1778) was based on various observations carried out at 25°C. The generation time was calculated to be 16.8 days; the intrinsic rate of natural increase was 0.12 per day, and the net reproductive rate was 7.2. A nonlinear function was found satisfactory to describe the developmental rate of the different immature lifestages at temperatures below 40°C. The stage-specific survival of the immature life-stages was generally high between 10 and 37°C, but decreased quickly outside this temperature range. Most of the eggs were laid during the first three weeks of the adult life. The proportion of surviving females rapidly decreases after moulting to the adult stage. At a temperature of 30°C, the highest number of eggs (3 eggs per day) was laid. 相似文献
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The D.M. Raup & A. Michelson model of assessing shell-coiling constitutes a method which simplifies the measuring of spiral-coiling gastropod shells. With, this method the information is obtained by measuring the photograph of the object.The newly developed method has the advantage of dispensing with an exact shell orientation (horizontally located axis) and magnification.Instead of photographing the object with a camera the shell is placed upon photographic paper and in this manner a contact showing the shell outlines can be achieved by exposing and developing the print. Correct information is then obtained by means of the conversion factors (1–3) as outlined in this paper. 相似文献
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Abstract Adaptation may be the result of a change in affinity and/or number of cAMP-binding sites at the cell surface. To test this possibility we used agip 53, a mutant that does not synthesize cAMP in response to cAMP stimulation. cAMP induced a fast decrease in cAMP-binding to aggregation-competent cells, which reached a maximum at 10–20 s and was reversible with a t 0.5 of about 70 s. The decrease in cAMP-binding involved 46000 sites per cell and was mainly due to a reduction in the apparent affinity for cAMP-binding and to a smaller extent to slowly dissociating cAMP. Our results suggest that under these conditions only a fraction of the cAMP-binding sites at the cell surface are involved in transmembrane signalling, which is indeed observed for many of the physiological responses in Dictyostelium discoideum . 相似文献
19.
Oscar K. K. Bedzo Kim Trollope Lalitha D. Gottumukkala Gerhardt Coetzee Johann F. Görgens 《Biotechnology progress》2019,35(3):e2797
The immobilization of β-fructofuranosidase for short-chain fructooligosaccharide (scFOS) synthesis holds the potential for a more efficient use of the biocatalyst. However, the choice of carrier and immobilization technique is a key to achieving that efficiency. In this study, calcium alginate (CA), Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) were tested as supports for immobilizing a novel engineered β-fructofuranosidase from Aspergillus japonicus for scFOS synthesis. Several immobilization parameters were estimated to ascertain the effectiveness of the carriers in immobilizing the enzyme. The performance of the immobilized biocatalysts are compared in terms of the yield of scFOS produced and reusability. The selection of carriers and reagents was motivated by the need to ensure safety of application in the production of food-grade products. The CA and AI900 both recorded impressive immobilization yields of 82 and 62%, respectively, while the DMM recorded 47%. Enzyme immobilizations on CA, AI900 and DMM showed activity recoveries of 23, 27, and 17%, respectively. The CA, AI900 immobilized and the free enzymes recorded their highest scFOS yields of 59, 53, and 61%, respectively. The AI900 immobilized enzyme produced a consistent scFOS yield and composition for 12 batch cycles but for the CA immobilized enzyme, only 6 batch cycles gave a consistent scFOS yield. In its first record of application in scFOS production, the AI900 anion exchange resin exhibited potential as an adequate carrier for industrial application with possible savings on cost of immobilization and reduced technical difficulty. 相似文献
20.
Johann Gasteiger 《生物产业技术》2008,(1):30-30
生物必须能在恶劣的环境中生存,为了达到这个目的,它们必须通过化学反应来产生能量以保持理想的温度,并且要代谢养料将其转化成代谢产物.最终转换成生物大分子。 相似文献