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41.
A detailed morphological staging system for cattle embryos at stages following blastocyst hatching and preceding gastrulation is presented here together with spatiotemporal mapping of gene expression for BMP4, BRACHYURY, CERBERUS1 (CER1), CRIPTO, EOMESODERMIN, FURIN and NODAL. Five stages are defined based on distinct developmental events. The first of these is the differentiation of the visceral hypoblast underlying the epiblast, from the parietal hypoblast underlying the mural trophoblast. The second concerns the formation of an asymmetrically positioned, morphologically recognisable region within the visceral hypoblast that is marked by the presence of CER1 and absence of BMP4 expression. We have termed this the anterior visceral hypoblast or AVH. Intra-epiblast cavity formation and the disappearance of the polar trophoblast overlying the epiblast (Rauber’s layer) have been mapped in relation to AVH formation. The third chronological event involves the transition of the epiblast into the embryonic ectoderm with concomitant onset of posterior NODAL, EOMES and BRACHYURY expression. Lastly, gastrulation commences as the posterior medial embryonic ectoderm layer thickens to form the primitive streak and cells ingress between the embryonic ectoderm and hypoblast. At this stage a novel domain of CER1 expression is seen whereas the AVH disappears. Comparison with the mouse reveals that while gene expression patterns at the onset of gastrulation are well conserved, asymmetry establishment, which relies on extraembryonic tissues such as the hypoblast and trophoblast, has diverged in terms of both gene expression and morphology. 相似文献
42.
Michelle van der Bank F. H. van der Bank B. -E. van Wyk 《Plant Systematics and Evolution》1999,219(1-2):27-38
We have tested the hypothesis that reseeding is a plesiomorphic character state and that sprouting is a derived state inAspalathus linearis, and that the latter is an adaptation to ensure fire-survival in a fireprone environment. Samples of five seeder and four sprouter populations of A.linearis were examined by horizontal starch gel electrophoresis to assess the amount of genetic differentiation within and between sprouting and seeding populations, and to determine the extent of gene flow between the populations. Leaf extracts were surveyed for ten enzymes and gene products revealed genetic variation at 13 (76%) of 17 protein coding loci. Allele frequency differences were found between sprouting and seeding populations and genetic distance values show that the sprouters are grouped separate from the seeders, thus providing support for the morphological data on which the above mentioned hypothesis is based. It is evident that evolution operates at the population level inA. linearis. 相似文献
43.
Jacob van Etten 《Journal of ethnobiology and ethnomedicine》2006,2(1):1-17
Small-scale studies on long-term change in agricultural knowledge might uncover insights with broader, regional implications. This article evaluates change in farmer knowledge about crop genetic resources in highland Guatemala between 1927/37 and 2004. It concentrates on maize (Zea mays ssp. mays L.) in one Guatemalan township, Jacaltenango, an area with much ecological and maize diversity. It relies on a particular type of baseline information: lists of farmer-defined cultivars drawn up by ethnographers in the first half of the twentieth century. A questionnaire format based on two independent lists of local farmer cultivars dating from 1927 and 1937 was used to assess changes in maize diversity. Comparisons between attributes given to each cultivar in the past and in 2004 were used as a partial test of the stability of cultivar identity. In farmers' perceptions, cultivar loss was low and limited to certain cultivars adapted to the warmer environments. Crop production problems were mentioned as the main motives for change. No evidence for a loss of cultivars due to the political violence of the 1980s was found. In the lower areas many newly introduced cultivars were found, which reportedly provide solutions for the production problems the older cultivars have. The article contrasts these findings with those of an earlier study which suggested much cultivar loss due to political violence, and draws conclusions about the methodological implications. 相似文献
44.
A comparison of the respiratory chain in particles from Paracoccus denitrificans and bovine heart mitochondria by EPR spectroscopy 总被引:6,自引:0,他引:6
S P Albracht H W van Verseveld W R Hagen M L Kalkman 《Biochimica et biophysica acta》1980,593(2):173-186
A study is presented on the EPR characteristics of the paramagnetic groups in the respiratory chain present in membrane particles of Paracoccus denitrificans, the respiratory system of which is very similar to that in submitochondrial particles from beef heart. All paramagnetic prosthetic groups of the mitochondrial system are also found in the bacterial plasma membrane. Their properties suggest that the respiratory groups are embedded in very similar protein environments in the two systems. 相似文献
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Hatchery selection promotes boldness in newly hatched brown trout (Salmo trutta): implications for dominance 总被引:2,自引:0,他引:2
Sundstrom L. Fredrik; Petersson Erik; Hojesjo Johan; Johnsson Jorgen I.; Jarvi Torbjorn 《Behavioral ecology》2004,15(2):192-198
By using newly hatched (approximately 2 weeks old) brown trout(Salmo trutta) from six families of wild and six families ofsea-ranched origin (seventh generation), we tested the hypothesesthat (1) the hatchery environment selects for increased boldness,and (2) boldness predicts dominance status. Sea-ranched troutspend their first 2 years in the hatchery before being releasedinto the wild at the onset of seaward migration. Trout werepresented with a novel object (tack) and with food (brine shrimp),and their responses were measured and scored in terms of boldness.Siblings with increasing difference in boldness were then pairedin dyadic contests. Fish of sea-ranged origin were on averagebolder than were fish of wild origin, and bolder individualswere more likely to become dominant regardless of origin. Boldnesswas not related to RNA levels, indicating that bold behaviorwas not a consequence of higher metabolism or growth rate. Neitherwas size a predictor of bold behavior or the outcome of dyadiccontests. These results are consistent with studies on olderlife stages showing increased boldness toward predators in hatchery-selectedfish, which suggests that behavioral consequences of hatcheryselection are manifested very early in life. The concordancebetween boldness and dominance may suggest that these behaviorsare linked in a risk prone-aggressive phenotype, which may bepromoted by hatchery selection. However, we also found significantvariation in behavioral and growth-related traits among families,suggesting that heritable variation has not been exhausted bysea-ranching procedures. 相似文献
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Two cases of right lower lobe oedema are presented as a supposedly very rare sign of left heart failure after myocardial infarction in the presence of acute mitral regurgitation. Causes of unilateral oedema and its clinical relevance in the diagnostic and therapeutic perspectives are discussed, and a review of the literature on this topic is presented. 相似文献
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