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991.
Abstract Multi component reactions (MCR) together with a suitable set of nucleobase building blocks provide a valuable tool for the fast generation of combinatorial libraries. These arrays of compounds are of high interest as anticancer and antiviral drugs. The preparation of small libraries and their analytics is described. 相似文献
992.
Marri Lönnberg Pertti Lehikoinen Rainer Käppi 《Nucleosides, nucleotides & nucleic acids》2013,32(1-2):397-398
Abstract Kinetics of the reactions of 6-substituted 9-(β-Q-ribofuranosyl)purines with aqueous alkalies have been studied liquid chromatographicaIly. 相似文献
993.
Abstract The detailed mechanisms of pH-independent cleavage and isomerization reactions of the phosphodiester bond are evaluated by examining kinetics of hydrolytic reactions of selected mono- and dialkyl esters of 3′-uridylic acid. The mechanistic choices are made by comparing LFER's between reaction rates and acidity of the alkyl group of phosphodi- and triesters. 相似文献
994.
Magdalena Eriksson Bengt Nordeh Bengt Jernström 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):717-720
Abstract Flow linear dichroism and fluorescence spectroscopy show that the covalent (+)-anti-BPDE-DNA complex adopts two rapidly interchanging conformations. The binding introduces local flexibility in DNA facilitating further covalent attacks. 相似文献
995.
A. Nyilas A. Földesi J. Chattopadhyaya 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):787-793
Abstract Phenyl-, 4-chlorophenyl- and 4-nitrophenylsufonylethoxycarbonyl groups have been reported for the first time as the exocyclic amino protecting groups in nucleoside chemistry. They are all stable under the standard conditions of manipulations in phosphotriester and phosphiteamidite chemistry, they are removable both under the alkaline hydrolytic conditions and also under the influence of non-nucleophilic tertiary bases. N3-Phenyl- and 4-toluenesulfonylethoxycarbonyl derivatives of uridine have been also prepared and characterized by 15N-NMR spectroscopy, their stabilities under different conditions have been tested. 相似文献
996.
Satu Mikkola Mikko Oivanen Kari Neuvonen Suvi Piitari Kaisa Ketomäki Harri Lönnberg 《Nucleosides, nucleotides & nucleic acids》2013,32(10-12):1675-1692
Abstract Uridylyl-(3′,5′)-8-carboxymethylaminoadenosine has been synthesised, and its transesterification to uridine 2′,3′-cyclic phosphate in the presence and absence of Zn2+ ion has been studied. The results show that a carboxylate function in the vicinity of the phosphodiester bond accelerates the metal ion promoted cleavage but not the metal ion independent reaction. Under acidic conditions, the predominant reaction is the cleavage of the side chain, giving the 8-amino derivative. 相似文献
997.
H. Seliger H. Fancelli G. Gröger S. Klein U. Kotschi R. Schlenk 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):645-650
Abstract Complete genes can now be prepared in single-batch procedures a) as one long oligonucleotide strand, b) as a number of simultaneously synthesized fragments. For further increase of overall efficiency DNA fragments terminated by a 3′-ribonucleoside can be joined by solid-phase single strand ligation using RNA ligase. This paper describes the application of these techniques to the preparation of parts of the human proopiomelanocortin gene. 相似文献
998.
Abstract A new plasmid which makes it possible to measure formation of a mature protein, as well as prematurely terminated gene products, has been constructed. It can be used for the analysis of translational efficiency of specified codon sequences in vivo. 相似文献
999.
S. Schlosser G. Gröger O. Holfeld H. Seliger 《Nucleosides, nucleotides & nucleic acids》2013,32(9-11):1515-1522
Abstract Using appropriately designed primers we amplified a 783 bp frag ment of the triple helical portion of human α1-collagen (I) by PCR methods. The expression of this biosynthetic collagen gene and a modified one containing cell adhesion sequences was carried out in a prokaryotic (E. coli) and a eukaryotic (baculovirus) system. The expression products were purified by affinity chromatography. 相似文献
1000.
Melanie Köllmer Carmen Popescu Prashanth Manda Leon Zhou Richard A. Gemeinhart 《AAPS PharmSciTech》2013,14(4):1333-1340
Pharmaceutical excipients contain reactive groups and impurities due to manufacturing processes that can cause decomposition of active drug compounds. The aim of this investigation was to determine if commercially available oral disintegrating tablet (ODT) platforms induce active pharmaceutical ingredient (API) degradation. Benzocaine was selected as the model API due to known degradation through ester and primary amino groups. Benzocaine was either compressed at a constant pressure, 20 kN, or at pressure necessary to produce a set hardness, i.e., where a series of tablets were produced at different compression forces until an average hardness of approximately 100 N was achieved. Tablets were then stored for 6 months under International Conference on Harmonization recommended conditions, 25°C and 60% relative humidity (RH), or under accelerated conditions, 40°C and 75% RH. Benzocaine degradation was monitored by liquid chromatography–mass spectrometry. Regardless of the ODT platform, no degradation of benzocaine was observed in tablets that were kept for 6 months at 25°C and 60% RH. After storage for 30 days under accelerated conditions, benzocaine degradation was observed in a single platform. Qualitative differences in ODT platform behavior were observed in physical appearance of the tablets after storage under different temperature and humidity conditions. 相似文献