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991.
AimWe incorporated genetic structure and life history phase in species distribution models (SDMs) constructed for a widespread spiny lobster, to reveal local adaptations specific to individual subspecies and predict future range shifts under the RCP 8.5 climate change scenario.LocationIndo‐West Pacific.MethodsMaxEnt was used to construct present‐day SDMs for the spiny lobster Panulirus homarus and individually for the three genetically distinct subspecies of which it comprises. SDMs incorporated both sea surface and benthic (seafloor) climate layers to recreate discrete influences of these habitats during the drifting larval and benthic juvenile and adult life history phases. Principle component analysis (PCA) was used to infer environmental variables to which individual subspecies were adapted. SDM projections of present‐day habitat suitability were compared with predictions for the year 2,100, under the RCP 8.5 climate change scenario.ResultsIn the PCA, salinity best explained P. h. megasculptus habitat suitability, compared with current velocity in P. h. rubellus and sea surface temperature in P. h. homarus. Drifting and benthic life history phases were adapted to different combinations of sea surface and benthic environmental variables considered. Highly suitable habitats for benthic phases were spatially enveloped within more extensive sea surface habitats suitable for drifting larvae. SDMs predicted that present‐day highly suitable habitats for P. homarus will decrease by the year 2,100.Main conclusionsIncorporating genetic structure in SDMs showed that individual spiny lobster subspecies had unique adaptations, which could not be resolved in species‐level models. The use of sea surface and benthic climate layers revealed the relative importance of environmental variables during drifting and benthic life history phases. SDMs that included genetic structure and life history were more informative in predictive models of climate change effects. 相似文献
992.
Quantitative trait locus (QTL) isogenic recombinant analysis: a method for high-resolution mapping of QTL within a single population 总被引:3,自引:0,他引:3 下载免费PDF全文
Peleman JD Wye C Zethof J Sørensen AP Verbakel H van Oeveren J Gerats T van der Voort JR 《Genetics》2005,171(3):1341-1352
In the quest for fine mapping quantitative trait loci (QTL) at a subcentimorgan scale, several methods that involve the construction of inbred lines and the generation of large progenies of such inbred lines have been developed (Complex Trait Consortium 2003). Here we present an alternative method that significantly speeds up QTL fine mapping by using one segregating population. As a first step, a rough mapping analysis is performed on a small part of the population. Once the QTL have been mapped to a chromosomal interval by standard procedures, a large population of 1000 plants or more is analyzed with markers flanking the defined QTL to select QTL isogenic recombinants (QIRs). QIRs bear a recombination event in the QTL interval of interest, while other QTL have the same homozygous genotype. Only these QIRs are subsequently phenotyped to fine map the QTL. By focusing at an early stage on the informative individuals in the population only, the efforts in population genotyping and phenotyping are significantly reduced as compared to prior methods. The principles of this approach are demonstrated by fine mapping an erucic acid QTL of rapeseed at a subcentimorgan scale. 相似文献
993.
Johan De Vogel-van den Bosch Sjoerd A.A. van den Berg Silvia BijlandPeter J. Voshol Louis M. Havekes Hans A. RomijnJoris Hoeks Denis van Beurden Matthijs K.C. HesselinkPatrick Schrauwen Ko Willems van Dijk 《The Journal of nutritional biochemistry》2011,22(4):366-371
Excess dietary long-chain fatty acid (LCFA) intake results in ectopic lipid accumulation and insulin resistance. Since medium-chain fatty acids (MCFA) are preferentially oxidized over LCFA, we hypothesized that diets rich in MCFA result in a lower ectopic lipid accumulation and insulin resistance compared to diets rich in LCFA. Feeding mice high-fat (HF) (45% kcal fat) diets for 8 weeks rich in triacylglycerols composed of MCFA (HFMCT) or LCFA (HFLCT) revealed a lower body weight gain in the HFMCT-fed mice. Indirect calorimetry revealed higher fat oxidation on HFMCT compared to HFLCT (0.011.0±0.0007 vs. 0.0096±0.0015 kcal/g body weight per hour, P<.05). In line with this, neutral lipid immunohistochemistry revealed significantly lower lipid storage in skeletal muscle (0.05±0.08 vs. 0.30±0.23 area%, P <.05) and in liver (0.9±0.4 vs. 6.4±0.8 area%, P<.05) after HFMCT vs. HFLCT, while ectopic fat storage in low fat (LF) was very low. Hyperinsulinemic euglycemic clamps revealed that the HFMCT and HFLCT resulted in severe whole body insulin resistance (glucose infusion rate: 53.1±6.8, 50.8±15.3 vs. 124.6±25.4 μmol min−1 kg−1, P<.001 in HFMCT, HFLCT and LF-fed mice, respectively). However, under hyperinsulinemic conditions, HFMCT revealed a lower endogenous glucose output (22.6±8.0 vs. 34.7±8.5 μmol min−1 kg−1, P<.05) and a lower peripheral glucose disappearance (75.7±7.8 vs. 93.4±12.4 μmol min−1 kg−1, P<.03) compared to HFLCT-fed mice. In conclusion, both HF diets induced whole body insulin resistance compared to LF. However, the HFMCT gained less weight, had less ectopic lipid accumulation, while peripheral insulin resistance was more pronounced compared to HFLCT. This suggests that HF-diets rich in medium- versus long-chain triacylglycerols induce insulin resistance via distinct mechanisms. 相似文献
994.
Facilitation in plant communities: the past, the present, and the future 总被引:24,自引:11,他引:24
Rob W. Brooker Fernando T. Maestre Ragan M. Callaway Christopher L. Lortie Lohengrin A. Cavieres Georges Kunstler Pierre Liancourt Katja Tielbörger Justin M. J. Travis Fabien Anthelme Cristina Armas Lluis Coll Emmanuel Corcket Sylvain Delzon Estelle Forey Zaal Kikvidze Johan Olofsson Francisco Pugnaire Constanza L. Quiroz Patrick Saccone Katja Schiffers Merav Seifan Blaize Touzard Richard Michalet 《Journal of Ecology》2008,96(1):18-34
995.
Mammalian embryonic stem cells have the potential to differentiate into all cell types of an adult individual. The culturing
of human embryonic stem cells renders possible studies that were previously only available in animal models. Embryonic stem
cells constitute a particularly attractive tool for studies of self-renewal, commitment, differentiation, maturation and cell-cell
interaction. There is currently considerable hope that studies of embryonic stem cells will lead to new therapies; either
by themselves, through cell replacement strategies, or by generating results assisting other fields of research to reach clinical
results. There are, however, considerable challenges to be met before embryonic stem cells can be used in large-scale clinical
trials.Stem cell research is an area that has given rise to much debate internationally, within science, law and politics
as well as within philosophy and ethics. The ethical attitudes expressed in the public debate over stem cell research notably
divide over three important distinctions: (1) Reproductive versus therapeutic cloning; (2) Using already existing embryos
versus producing new embryos for research purposes; (3) Production of embryos from eggs and sperm versus through somatic-cell
nuclear transfer. The potential medical benefits that may result from embryonic stem cell research arguably support a continued
development in this area. However, some opponents argue that this research offends the (relative or absolute) moral status
of an unborn human. Furthermore, the research would probably prove to be a both time-consuming and very expensive method for
treating disease. Thus, the questions arise whom the new technique wouldbenefit and at what cost, if ever developed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
996.
Juhwan Lee Gabriel Pedroso Bruce A. Linquist Daniel Putnam Chris van Kessel Johan Six 《Global Change Biology Bioenergy》2012,4(5):521-533
The production potential of switchgrass (Panicum virgatum L.) has not been estimated in a Mediterranean climate on a regional basis and its economic and environmental contribution as a biofuel crop remains unknown. The objectives of the study were to calibrate and validate a biogeochemical model, DAYCENT, and to predict the biomass yield potential of switchgrass across the Central Valley of California. Six common cultivars were calibrated using published data across the US and validated with data generated from four field trials in California (2007–2009). After calibration, the modeled range of yields across the cultivars and various management practices in the US (excluding California) was 2.4–41.2 Mg ha?1 yr?1, generally compatible with the observed yield range of 1.3–33.7 Mg ha?1 yr?1. Overall, the model was successfully validated in California; the model explained 66–90% of observed yield variation in 2007–2009. The range of modeled yields was 2.0–41.4 Mg ha?1 yr?1, which corresponded to the observed range of 1.3–41.1 Mg ha?1 yr?1. The response to N fertilizer and harvest frequency on yields were also reasonably validated. The model estimated that Alamo (21–23 Mg ha?1 yr?1) and Kanlow (22–24 Mg ha?1 yr?1) had greatest yield potential during the years after establishment. The effects of soil texture on modeled yields tended to be consistent for all cultivars, but there were distinct climatic (e.g., annual mean maximum temperature) controls among the cultivars. Our modeled results suggest that early stand maintenance of irrigated switchgrass is strongly dependent on available soil N; estimated yields increased by 1.6–5.5 Mg ha?1 yr?1 when residual soil mineral N was sufficient for optimal re‐growth. Therefore, management options of switchgrass for regional biomass production should be ecotype‐specific and ensure available soil N maintenance. 相似文献
997.
Ping-Pin Zheng Rob Willemsen Johan M. Kros 《Biochemical and biophysical research communications》2009,380(4):823-824
Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be used for portraying of functional vasculature in experimental zebrafish embryos. The staining sufficiently revealed aortic arches, dorsal aorta, posterior cardinal vein, dorsal longitudinal anastomotic vessels, intersegmental vessels as well as subintestinal vessel basket. We conclude that Hb staining offers an informative and rapid method for in vivo portraying of functional vasculature in experimental zebrafish embryos. It is also suitable for large scale experiments. 相似文献
998.
Claus Rueffler Johan A. J. Metz Tom J. M. Van Dooren 《Journal of mathematical biology》2013,66(1-2):225-279
We analyze long-term evolutionary dynamics in a large class of life history models. The model family is characterized by discrete-time population dynamics and a finite number of individual states such that the life cycle can be described in terms of a population projection matrix. We allow an arbitrary number of demographic parameters to be subject to density-dependent population regulation and two or more demographic parameters to be subject to evolutionary change. Our aim is to identify structural features of life cycles and modes of population regulation that correspond to specific evolutionary dynamics. Our derivations are based on a fitness proxy that is an algebraically simple function of loops within the life cycle. This allows us to phrase the results in terms of properties of such loops which are readily interpreted biologically. The following results could be obtained. First, we give sufficient conditions for the existence of optimisation principles in models with an arbitrary number of evolving traits. These models are then classified with respect to their appropriate optimisation principle. Second, under the assumption of just two evolving traits we identify structural features of the life cycle that determine whether equilibria of the monomorphic adaptive dynamics (evolutionarily singular points) correspond to fitness minima or maxima. Third, for one class of frequency-dependent models, where optimisation is not possible, we present sufficient conditions that allow classifying singular points in terms of the curvature of the trade-off curve. Throughout the article we illustrate the utility of our framework with a variety of examples. 相似文献
999.
1000.
Parallel gene analysis with allele-specific padlock probes and tag microarrays 总被引:9,自引:3,他引:9 下载免费PDF全文
Banér J Isaksson A Waldenström E Jarvius J Landegren U Nilsson M 《Nucleic acids research》2003,31(17):e103
Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory technique suitable to analyze numerous target sequences in multiplexed assays. Sets of padlock probes were applied to analyze single nucleotide variation directly in total genomic DNA or cDNA for parallel genotyping or gene expression analysis. All reacted probes were then co-amplified and identified by hybridization to a standard tag oligonucleotide array. The technique was illustrated by analyzing normal and pathogenic variation within the Wilson disease-related ATP7B gene, both at the level of DNA and RNA, using allele-specific padlock probes. 相似文献