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31.
Abstract— The NH2-terminal amino acids of Wolfgram and Folch-Lees proteolipids of bovine and human CNS myelin were determined using the cyanate method (Starke & Smyth , 1963) followed by direct amino acid analysis of the products. Glycine predominated in every case and was recovered in amounts similar to the results described by Whikehart & Lees (1973), who used a dansylation technique followed by thin layer chromatography of the DNS-amino acids. In the present study substantial amounts of glutamic acid, serine, alanine and aspartic acid were also recovered, plus traces of other amino acids. Few differences were observed between Wolfgram and Folch-Lees proteolipids. The end group products of purified W1 proteolipid of bovine Wolfgram fraction, of diazometholysed Folch-Lees proteolipid, and of a sample of phosphatidyl serine had essentially the same composition. The similarity of these results, especially for both fractionated and unfractionated Wolfgram proteolipid, may be evidence that the observed products are derived from phosphoglycerides present in proteolipid rather than from the actual NH2-terminals of the protein. 相似文献
32.
H C Seftel S G Baker M P Sandler M B Forman B I Joffe D Mendelsohn T Jenkins C J Mieny 《BMJ (Clinical research ed.)》1980,281(6241):633-636
From 1972 to 1979 34 patients with homozygous familial hypercholesterolaemia were seen in one clinic in Johannesburg. All were Afrikaners and most lived in Transvaal Province. Their epidemiological, genetic, clinical, and biochemical characteristics were studied. The course of the disease varied considerably among the 34 patients, with no fewer than six surviving into their fourth or fifth decades. In some patients arterial atheroma was severe while cutaneotendinous xanthomas were slight and vice versa. Coronary heart disease was common but peripheral and cerebral arterial disease was rare. Another prominent finding was high concentrations of low-density lipoprotein cholesterol coupled with low high-density lipoprotein cholesterol values. The prevalences of homozygotes and heterozygotes with familial hypercholesterolaemia in Transvaal Afrikaners, calculated from this group of patients, were 1 in 30,000 and 1 in 100 respectively. These figures are the highest ever reported and may help to explain why South African whites have the highest death rate from coronary heart disease in the Western world. 相似文献
33.
A. Z. Joffe 《Mycopathologia》1969,37(2):150-160
Summary A comparative study was carried out in groundnut fields in Israel of the mycoflora of rhizosphere (R) and soil adhering to geocarps (G) and of the soil (S).Of a total of 157 fungal species isolated and listed, 133 occurred in R, 96 in S, and 86 in G. Ten species considered rare or worthy of special note are briefly discussed.Species ofAspergillus were most numerous in heavy soil, species ofPenicillium in light soil, and species ofFusarium in medium soil.Penicillium funiculosum andP. rubrum were the species occurring with greatest frequency, and these were equally common in R, S, and G of light and medium soil. Soil inoculation withA. flavus depressed the number of species in the mycoflora of R and S.Quantitative comparison of R, S, and G on the three soils showed that numbers for R exceeded those for S and G, forP. funiculosum andP. rubrum on all soils, and for several Aspergilli on heavy soil. Among fungi more prevalent in S than in R and G wasA. flavus on medium and heavy soil. No special affinity of any one of the 3 major groups (Aspergillus, Penicillium, Fusarium) for the mycoflorae of R or S or G has been found. The relative number of colonies of all fungi in R and S (R/S ratio) was in almost all cases in excess of 1 on all soil types.This research is supported by grant number FG-161 of the United States Department of Agriculture, to whom the author is indebted. 相似文献
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Ordered arrangement and rearrangement of chromosomes during spermatogenesis in two species of planarians (Plathelminthes) 总被引:3,自引:0,他引:3
The pattern of distribution of telomeric DNA (TTAGGG), 28S rDNA, and 5S rDNA has been studied using fluorescence in situ
hybridization (FISH) and primed in situ labelling during spermatogenesis and sperm formation in the filiform spermatozoa of
two species of planarians, Dendrocoelum lacteum and Polycelis tenuis (Turbellaria, Plathelminthes). In both species, the positions of FISH signals found with each probe sequence are constant
from cell to cell in the nuclei of mature sperm. Chromosome regions containing 5S and 28S rDNA genes are gathered in distinct
bundles of spiral form. In early spermatids with roundish nuclei, the sites of a given sequence on different chromosomes remain
separate. Centromeres (marked by 5S rDNA) gather into a single cluster in the central region of the slightly elongated sperm
nucleus. During spermatid maturation, this cluster migrates to the distal pole of the nucleus. In Polycelis, telomeric sites gather into three distinct clusters at both ends and in the middle of the moderately elongated nucleus.
These clusters retain their relative positions as the spermatid matures. All the chromosome ends bearing 28S rDNA gather only
into the proximal cluster. Our data suggest that structures in the nucleus selectively recognise chromosome regions containing
specific DNA sequences, which helps these regions to find their regular places in the mature sperm nucleus and causes clustering
of the sites of these sequences located on different chromosomes. This hypothesis is supported by observations on elongated
sperm of other animals in which a correlation exists between ordered arrangement of chromosomes in the mature sperm nucleus
and clustering of sites of the same sequence from different chromosomes during spermiogenesis.
Received: 15 December 1997; in revised form: 24 March 1998 / Accepted: 14 April 1998 相似文献
39.
Neuroscience research on sex difference is currently a controversial field, frequently accused of purveying a ‘neurosexism’ that functions to naturalise gender inequalities. However, there has been little empirical investigation of how information about neurobiological sex difference is interpreted within wider society. This paper presents a case study that tracks the journey of one high-profile study of neurobiological sex differences from its scientific publication through various layers of the public domain. A content analysis was performed to ascertain how the study was represented in five domains of communication: the original scientific article, a press release, the traditional news media, online reader comments and blog entries. Analysis suggested that scientific research on sex difference offers an opportunity to rehearse abiding cultural understandings of gender. In both scientific and popular contexts, traditional gender stereotypes were projected onto the novel scientific information, which was harnessed to demonstrate the factual truth and normative legitimacy of these beliefs. Though strains of misogyny were evident within the readers’ comments, most discussion of the study took pains to portray the sexes’ unique abilities as equal and ‘complementary’. However, this content often resembled a form of benevolent sexism, in which praise of women’s social-emotional skills compensated for their relegation from more esteemed trait-domains, such as rationality and productivity. The paper suggests that embedding these stereotype patterns in neuroscience may intensify their rhetorical potency by lending them the epistemic authority of science. It argues that the neuroscience of sex difference does not merely reflect, but can actively shape the gender norms of contemporary society. 相似文献
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