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151.
An experiment designed to generate a wide variety of epidemics of Mycosphaerella graminicola without deliberate replication was done in two years. Disease severity was estimated at frequent intervals during the life of the crop and yields measured at harvest. Absolute estimates of disease severity were derived by regression of visual estimates on subsamples of leaves on which severity was estimated objectively using an image analysis system. Yield was predicted best by the integral of the square root of M. graminicola severity over the normal lifetime of each leaf, measured in thermal time. Only the top two leaves contributed to yield loss; no influence of the third leaf on yield was detected. Thousand grain weight was best predicted by the integral of the square root of M. graminicola severity on the flag leaf alone. Parameter estimates were similar in the two years. The prediction equations were consistent with yields observed in an experiment done in a third year using two sowing dates and two rates of nitrogen fertilisation, despite a much greater range of disease severity. Although critical point models could describe each year's results adequately, neither parameter estimates nor the growth stage at which the best relation occurred were consistent across years. The equations to predict loss may be useful for farmers in decision-support systems which are based on prediction. 相似文献
152.
Ibtissam Echchgadda Jody C. Cantu Joey Butterworth Bryan Gamboa Ronald Barnes David A. Freeman Francis A. Ruhr Weston C. Williams Leland R. Johnson Jason Payne Robert J. Thomas William P. Roach Bennett L. Ibey 《Bioelectromagnetics》2023,44(1-2):5-16
Previous research has shown that virus infectivity can be dramatically reduced by radio frequency exposure in the gigahertz (GHz) frequency range. Given the worldwide SARS-CoV-2 pandemic, which has caused over 1 million deaths and has had a profound global economic impact, there is a need for a noninvasive technology that can reduce the transmission of virus among humans. RF is a potential wide area-of-effect viral decontamination technology that could be used in hospital rooms where patients are expelling virus, in grocery and convenience stores where local populations mix, and in first responder settings where rapid medical response spans many potentially infected locations within hours. In this study, we used bovine coronavirus (BCoV) as a surrogate of SARS-CoV-2 and exposed it to high peak power microwave (HPPM) pulses at four narrowband frequencies: 2.8, 5.6, 8.5, and 9.3 GHz. Exposures consisted of 2 µs pulses delivered at 500 Hz, with pulse counts varied by decades between 1 and 10,000. The peak field intensities (i.e. the instantaneous power density of each pulse) ranged between 0.6 and 6.5 MW/m2, depending on the microwave frequency. The HPPM exposures were delivered to plastic coverslips containing BCoV dried on the surface. Hemagglutination (HA) and cytopathic effect analyses were performed 6 days after inoculation of host cells to assess viral infectivity. No change in viral infectivity was seen with increasing dose (pulse number) across the tested frequencies. Under all conditions tested, exposure did not reduce infectivity more than 1.0 log10. For the conditions studied, high peak power pulsed RF exposures in the 2–10 GHz range appear ineffective as a virucidal approach for hard surface decontamination. © 2023 Bioelectromagnetics Society. 相似文献
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Plants surrounding a gap in stand compensated to some extent for leaf yield lost to missing plants. The contribution of plants in an adjacent row was much less than that of plants next to the gap within the row. Losses were reduced and quality was improved by replacing destroyed plants within two weeks of the original planting. 相似文献
154.
Yakov Chudnovsky Joey F. Li Peter J. Rizzo J. Woodland Hastings Thomas F. Fagan 《Journal of phycology》2002,38(3):543-550
Although nucleosomes and histones are lacking in dinoflagellate nuclei, small basic histone‐like proteins have been reported, but their function(s) is unknown. In this study we cloned and sequenced a gene for a histone‐like protein from the dinoflagellate Lingulodinium polyedrum (Stein) Dodge (HLp) (formerly Gonyaulax polyedra Stein) and investigated its post‐translational modification and DNA‐binding activities. HLp appears to be acetylated in L. polyedrum, and we identified several L. polyedrum proteins that possess histone acetyltransferase activity and may be responsible for this modification. HLp binds weakly to L. polyedrum DNA but to certain specific sequences with higher affinity, consistent with its having a regulatory function. 相似文献
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Growth hormone (GH) and insulin-like growth factor I (IGF-I) are expressed in specific regions of the central nervous system during early human development. They may consequently influence aspects of cognition, or emotional and behavioural adjustment from childhood to adulthood, in conditions associated with abnormalities of the somatotropic axis. GH receptors are relatively common within hippocampal and perihippocampal regions that are primarily involved in declarative memory for facts and events. They are also located in structures (e.g. the putamen) that are involved in the processing of social perceptions. IGF-I receptors have been discovered in the amygdala and prefrontal cortex, which contribute to the neural circuits known as the 'social brain'. The evaluation of emotional, social and behavioural adjustment among children who have deficiencies in GH or IGF-I functional integrity requires the objective assessment of their social-cognitive competence. We describe a computerized test battery, the Schedules for the Assessment of Social Intelligence (SASI), which has been shown to possess excellent psychometric properties in terms of reliability and validity. The SASI, which can be used by both children and adults, may provide new evidence for deficits and treatment effects of GH/IGF-I on emotional, behavioural and cognitive functions. 相似文献
157.
Bernhard OK Lai J Wilkinson J Sheil MM Cunningham AL 《The Journal of biological chemistry》2004,279(50):51828-51835
DC-SIGN (dendritic cell specific intracellular adhesion molecule 3 grabbing non-integrin) or CD209 is a type II transmembrane protein and one of several C-type lectin receptors expressed by dendritic cell subsets, which bind to high mannose glycoproteins promoting their endocytosis and potential degradation. DC-SIGN also mediates attachment of HIV to dendritic cells and binding to this receptor can subsequently lead to endocytosis or enhancement of CD4/CCR5-dependent infection. The latter was proposed to be facilitated by an interaction between DC-SIGN and CD4. Endocytosis of HIV virions does not necessarily lead to their complete degradation. A proportion of the virions remain infective and can be later presented to T cells mediating their infection in trans. Previously, the extracellular domain of recombinant DC-SIGN has been shown to assemble as tetramers and in the current study we use a short range covalent cross-linker and show that DC-SIGN exists as tetramers on the surface of immature monocyte-derived dendritic cells. There was no evidence of direct binding between DC-SIGN and CD4 either by cross-linking or by fluorescence resonance energy transfer measurements suggesting that there is no constitutive association of the majority of these proteins in the membrane. Importantly we also show that the tetrameric complexes, in contrast to DC-SIGN monomers, bind with high affinity to high mannose glycoproteins such as mannan or HIV gp120 suggesting that such an assembly is required for high affinity binding of glycoproteins to DC-SIGN, providing the first direct evidence that DC-SIGN tetramers are essential for high affinity interactions with pathogens like HIV. 相似文献
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