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991.
992.
Rémy Moisant Elliott Cowles Léonie Broutel Véronique Deparis Anthony Baud Joerg von Hagen Harald Kolmar 《Biotechnology progress》2023,39(5):e3372
A challenging aspect with the use of the Sp2/0 hybridoma cell line in commercial manufacturing processes of recombinant therapeutic proteins is their exogenous lipids requirement for cell proliferation and optimal protein secretion. Lipids are commonly provided to the culture using serum or serum-derivatives, such as lipoprotein supplement. The batch-to-batch variability of these non-chemically defined raw-materials is known to impact cell culture process performance. Lipoprotein supplement variability and its impact on fed-batch production of a recombinant monoclonal antibody (mAb) expressed in Sp2/0 cells were studied using 36 batches from the same vendor. Several batches were associated with early viability drops leading to low process performance during fed-batch production. Increased caspase-3 activity (an indicator of apoptosis) was correlated to viability drops when low-performing batches were used. Addition of an antioxidant to the culture limited the increase in caspase-3 activity. Physicochemical characterization of batches confirmed that lipoproteins are mainly composed of lipids and proteins; no clear correlation between low-performing batches and lipoprotein supplement composition was observed. Controlled lipoprotein oxidation leads to lipoprotein solution browning, increasing absorbance at 276 nm and results in poor process performance. Because low-performing batches absorb more at 276 nm than other batches, oxidized lipids were suspected to be the root cause of low-performing batches. This study increased the understanding of lipoprotein supplement composition, its sensitivity to oxidation and its impact on process performance. 相似文献
993.
Cornelia E. den Heyer W. Don Bowen Julian Dale Jean-François Gosselin Michael O. Hammill David W. Johnston Shelley L. C. Lang Kimberly T. Murray Garry B. Stenson Stephanie A. Wood 《Marine Mammal Science》2021,37(2):611-630
The northwest Atlantic subspecies of gray seal (Halicheorus grypus grypus) has been increasing for more than a half century and has reestablished breeding colonies in Canadian and US waters. In 2016, visual, oblique, and vertical large-format digital photographic surveys were conducted at all known breeding colonies in the northwest Atlantic. Total pup production in the northwest Atlantic was estimated to be 109,000 (SE = 17,500) pups. At 87,500 (SE = 15,100) pups, Sable Island accounts for 80% of total pup production. Regional differences in pup production trends are evident. Pup production in the Gulf of St. Lawrence and along the eastern shore of Nova Scotia has been relatively stable. Since 2004, the rate of increase in pup production at Sable Island has slowed to about 5%–7% per year, while the newer colonies in southwest Nova Scotia and the northeastern United States are increasing rapidly. In 2016, the Muskeget Island (MA) breeding colony produced 3,900 (SE = 200) pups, making it the third largest breeding colony in the northwest Atlantic. This southward shift in production may reflect climate-mediated changes in population growth as well as reestablishment of colonies throughout the former range associated with increased protection. 相似文献
994.
Biomass for bioenergy is debated for its potential synergies or tradeoffs with other provisioning and regulating ecosystem services (ESS). This biomass may originate from different production systems and may be purposefully grown or obtained from residues. Increased concerns globally about the sustainable production of biomass for bioenergy has resulted in numerous certification schemes focusing on best management practices, mostly operating at the plot/field scale. In this study, we compare the ESS of two watersheds in the southeastern US. We show the ESS tradeoffs and synergies of plantation forestry, i.e., pine poles, and agricultural production, i.e., wheat straw and corn stover, with the counterfactual natural or semi-natural forest in both watersheds. The plantation forestry showed less distinct tradeoffs than did corn and wheat production, i.e., for carbon storage, P and sediment retention, groundwater recharge, and biodiversity. Using indicators of landscape composition and configuration, we showed that landscape planning can affect the overall ESS supply and can partly determine if locally set environmental thresholds are being met. Indicators on landscape composition, configuration and naturalness explained more than 30% of the variation in ESS supply. Landscape elements such as largely connected forest patches or more complex agricultural patches, e.g., mosaics with shrub and grassland patches, may enhance ESS supply in both of the bioenergy production systems. If tradeoffs between biomass production and other ESS are not addressed by landscape planning, it may be reasonable to include rules in certification schemes that require, e.g., the connectivity of natural or semi-natural forest patches in plantation forestry or semi-natural landscape elements in agricultural production systems. Integrating indicators on landscape configuration and composition into certification schemes is particularly relevant considering that certification schemes are governance tools used to ensure comparable sustainability standards for biomass produced in countries with variable or absent legal frameworks for landscape planning. 相似文献
995.
Alexander W. Fischer Michelle Y. Jaeckstein Kristina Gottschling Markus Heine Frederike Sass Nils Mangels Christian Schlein Anna Worthmann Oliver T. Bruns Yucheng Yuan Hua Zhu Ou Chen Harald Ittrich Stefan K. Nilsson Patrik Stefanicka Jozef Ukropec Miroslav Balaz Hua Dong Joerg Heeren 《Cell metabolism》2021,33(3):547-564.e7
996.
Rosolino Bumbalo Marilena Lieber Lisa Schroeder Yasemin Polat Heinz Breer Joerg Fleischer 《Cellular and molecular neurobiology》2017,37(4):729-742
Neurons of the Grueneberg ganglion respond to cool temperatures as well as to distinct odorants and extend axonal processes to the olfactory bulb of the brain. Analyses of transgenic mice, in which Grueneberg ganglion neurons and their axons are labeled, revealed that these axons innervated nine distinct glomeruli distributed in a characteristic topographical pattern in dorsal, lateral, ventral, and medial regions of rather posterior areas in the bulb. To assess activation of these glomeruli (hereinafter designated as Grueneberg glomeruli) upon stimulation of Grueneberg ganglion neurons, mice were exposed to the odorant 2,3-dimethylpyrazine (2,3-DMP) and the expression of the activity-dependent marker c-Fos in juxtaglomerular cells of the relevant glomeruli was monitored. It was found that all of these glomeruli were activated, irrespective of their localization in the bulb. To verify that the activation of juxtaglomerular cells in Grueneberg glomeruli was indeed based on stimulation of Grueneberg ganglion neurons, the 2,3-DMP-induced responses in these glomeruli were investigated in mice lacking the cyclic nucleotide-gated channel CNGA3 which is critical for chemo- and thermosensory signal transduction in Grueneberg ganglion neurons. This approach revealed that elimination of CNGA3 led to a reduction of the odorant-induced activity in Grueneberg glomeruli, indicating that the activation of these glomeruli is based on a preceding stimulation of the Grueneberg ganglion. Analyzing whether Grueneberg glomeruli in the bulb might also process thermosensory information, it was found that upon exposure to coolness, Grueneberg glomeruli were activated. Investigating mice lacking CNGA3, the activation of these glomeruli by cool temperatures was attenuated. 相似文献
997.
998.
999.
Gaëtan Burgio Michel Baylac Evelyne Heyer Xavier Montagutelli 《Evolution; international journal of organic evolution》2009,63(10):2668-2686
To assess the genetic basis of the skull shape variation and morphological integration in mice, we have used a tool based on the cross between the distantly related mouse species Mus spretus (SEG/Pas strain) and the laboratory strain C57BL/6 called interspecific recombinant congenic strains (IRCSs). The genome of each IRCS consists on average of 1.3% of SEG/Pas derived sequences, located on multiple chromosomes as small-sized, DNA segments. Quantitative trait loci (QTL) on the skull shape, separated into dorsal and ventral sides, were analyzed in 17 IRCSs by a Procrustes superimposition method using 3D landmarks. The shapes of 16 strains differed significantly from C57BL/6. Discrepancy in the QTLs effects was found between the dorsal side and the anterior region of the ventral side due to a differential effect of the SEG/Pas alleles on the skull shape. A comprehensive analysis of all allelic combinations of the BCG-66H strain showed strong epistatic interactions between SEG/Pas segment acting on both skull sides. Epistatic pleiotropy and covariation between sides were dependent in SEG/Pas alleles direction and contributed to the strong morphological integration between sides. Introduction of Mus spretus alleles in a C57BL/6 background induced strong morphological changes mostly in SEG/Pas alleles direction and provided evidence for high level of morphological integration. 相似文献
1000.
Weise Ellen M. Van Wyngaarden Mallory Den Heyer Cornelia Mills Flemming Joanna Kess Tony Einfeldt Anthony L. Fisher Jonathan A. D. Ditta Reina Pare Guillaume Ruzzante Daniel E. 《Marine biotechnology (New York, N.Y.)》2023,25(4):580-587
Marine Biotechnology - The ability to identify sex is necessary in population biology for a proper understanding of the dynamics of a population. In Atlantic halibut, phenotypic sex identification... 相似文献