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Jonathan D. Choi Lara A. Underkoffler Joelle N. Collins Shannon M. Marchegiani Natalie A. Terry Colin V. Beechey Rebecca J. Oakey 《Mammalian genome》2001,12(10):758-764
Microarray analysis allows the screening of thousands of identifiable genes in a single experiment. The challenge of this
approach is to combine the new technology with established genetic tools to associate genes with specific biological function.
In this study we have designed a screen to identify imprinted genes from mice with uniparental duplications of proximal Chromosomes
(Chrs) 7 and 11, using microarray analysis. By comparing the expression patterns in embryonic and newborn tissues of maternally
versus paternally inherited proximal Chrs 7 and 11, we have correctly identified four out of five known imprinted genes represented
on a microarray. We have additionally identified two novel imprinted candidate genes as well as a differentially expressed
clone that is a potential downstream target. Interpretation of the microarray data requires careful preparation of age- and
strain-matched samples and attention to detail in tissue dissection technique.
Received: 15 March 2001 / Accepted: 13 June 2001 相似文献
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Identified, proliferating S-phase cells in the postembryonic fish ear are known to be the precursors to new hair cells. It is not known, however, whether the ability to proliferate is restricted to a small population of cells. The ability of cells that are not normally in the cell cycle to enter S-phase was examined using the antimitotic drug cytosine arabinoside (ara-C). The normal population of S-phase cells in the saccule was destroyed by a single large dose of ara-C. Two weeks later, the prsence of S-phase cells was evaluated using the S-phase marker bromodeoxyuridine. The results strikingly demonstrate that S-phase cells are replaced, since S-phase cells returned to the saccule in the same number as found in normal fish. The data are interpreted to suggest that a large number of nonsensory support cells are capable of entering the cell cycle and that some mechanism must regulate which of these are actually cycling at any given time. © 1995 John Wiley & Sons, Inc. 相似文献