全文获取类型
收费全文 | 2394篇 |
免费 | 219篇 |
国内免费 | 2篇 |
专业分类
2615篇 |
出版年
2023年 | 10篇 |
2022年 | 25篇 |
2021年 | 44篇 |
2020年 | 27篇 |
2019年 | 34篇 |
2018年 | 35篇 |
2017年 | 32篇 |
2016年 | 47篇 |
2015年 | 102篇 |
2014年 | 105篇 |
2013年 | 133篇 |
2012年 | 175篇 |
2011年 | 173篇 |
2010年 | 112篇 |
2009年 | 74篇 |
2008年 | 143篇 |
2007年 | 124篇 |
2006年 | 122篇 |
2005年 | 135篇 |
2004年 | 136篇 |
2003年 | 137篇 |
2002年 | 126篇 |
2001年 | 33篇 |
2000年 | 28篇 |
1999年 | 31篇 |
1998年 | 19篇 |
1997年 | 26篇 |
1996年 | 23篇 |
1995年 | 21篇 |
1994年 | 14篇 |
1993年 | 32篇 |
1992年 | 19篇 |
1991年 | 18篇 |
1990年 | 13篇 |
1989年 | 10篇 |
1988年 | 13篇 |
1987年 | 10篇 |
1986年 | 15篇 |
1985年 | 20篇 |
1984年 | 18篇 |
1983年 | 11篇 |
1982年 | 27篇 |
1981年 | 16篇 |
1980年 | 17篇 |
1979年 | 9篇 |
1978年 | 11篇 |
1976年 | 11篇 |
1975年 | 12篇 |
1974年 | 9篇 |
1973年 | 13篇 |
排序方式: 共有2615条查询结果,搜索用时 15 毫秒
1.
Burt V. Bronk Joe D. Patton David N. Mellard 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1982,697(3):278-285
Chick embryo fibroblasts were treated with the monofunctional alkylating agent methylmethane sulfonate at various concentrations for 1 h at 42°C, rinsed and then incubated post-treatment at various temperatures at which the kinetics of alkali-labile bond disappearance was followed. Growth experiments showed that these cells grew similarly at temperatures of either 37°C or 42°C. Repair as assessed by removal of alkali-labile bond was also similar for postincubation in the temperature range 37–42°C for damage due to methylmethane sulfonate treatment at concentrations less than 1.5 mM. When the postincubation temperature was raised higher than 42.5–43°C, this type of repair was stopped. The normal internal body temperature of adult chickens is about 41.6°C. Hence the present finding indicates that chick cells are much more severely restricted in DNA repair at temperatures above normal than are mammalian cells, which can function in this respect for several deg. C above 37°C. 相似文献
2.
Joe T. Ritchie 《Plant and Soil》1981,58(1-3):81-96
3.
4.
Repair of benzo[a]pyrene-initiated DNA damage in human cells requires activation of DNA polymerase alpha 总被引:1,自引:0,他引:1
Normal human fibroblasts treated with r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) yielded DNA polymerase alpha with elevated levels of activity, incorporated [3H]thymidine as a function of unscheduled DNA synthesis, and exhibited restoration of normal DNA-strand length as a function of unscheduled DNA synthesis. Lipoprotein-deficient fibroblasts treated with BPDE did not show elevated levels of DNA polymerase alpha activity, exhibited minimal [3H]thymidine incorporation, and had fragmented DNA after 24 h of repair in the absence of lipoprotein or phosphatidylinositol supplementation. When DNA polymerase beta activity was inhibited, cells with normal lipoprotein uptake exhibited [3H]thymidine incorporation into BPDE-damaged DNA but did not show an increase in DNA-strand length. DNA polymerase alpha activity and [3H]thymidine incorporation in lipoprotein-deficient fibroblasts increased to normal levels when the cells were permeabilized and low-density lipoproteins or phosphatidylinositol were introduced into the cells. DNA polymerase alpha isolated from normal human fibroblasts, but not from lipoprotein-deficient fibroblasts, showed increased specific activity after the cells were treated with BPDE. When BPDE-treated lipoprotein-deficient fibroblasts were permeabilized and 32P-ATP was introduced into the cells along with lipoproteins, 32P-labeled DNA polymerase alpha with significantly increased specific activity was isolated from the cells. These data suggest that treatment of human fibroblasts with BPDE initiates unscheduled DNA synthesis, as a function of DNA excision repair, which is correlated with increased activity of DNA polymerase alpha, and that increased DNA polymerase alpha activity may be correlated with phosphorylation of the enzyme in a reaction that is stimulated by low-density lipoprotein or by the lipoprotein component, phosphatidylinositol. 相似文献
5.
6.
Terry Joe Sprinkle F. Arthur McMorris Jun Yoshino George H. De Vries 《Neurochemical research》1985,10(7):919-931
The relative levels of the central nervous system myelin marker enzyme 2:3-cyclic nucleotide 3-phosphodiesterase (EC 3.1.4.37, CNPase) were determined in neuroblastoma, astrocyte, oligodendrocyte and Schwann cell cultures and in freshly isolated human lymphocytes and platelets. The highest specific activities were associated with the cells that elaborate myelin membrane in the central and peripheral nervous system, oligodendrocytes and Schwann cells, respectively. Antiserum to bovine CNPase recognized both CNP1 and CNP2 in CNS myelin and human oligodendroglioma. In addition, a 53,000 dalton protein was evident on autoradiographs of immunoblotted PNS myelin and human oligodendroglioma proteins. Cultured rat oligodendrocyte, C6 and mouse NA neuroblastoma CNPase appear to share common determinants with the corresponding normal rat CNS enzyme. 相似文献
7.
Human lymphocytes which were non-mitogen-stimulated, and which were depleted of lipoproteins, were found to be deficient in DNA excision repair typically initiated in these cells in response to treatment with a direct-acting polynuclear aromatic hydrocarbon carcinogen. Lymphocytes either depleted of lipoproteins or supplemented with human low-density lipoproteins formed DNA-carcinogen adducts which were not chromatographically distinguishable. The state of lipoprotein depletion did not alter lymphocyte uptake of thymidine from the medium. Lymphocytes which were depleted of lipoproteins, treated with carcinogen, and subsequently supplemented with low-density lipoproteins, regained the ability to engage in DNA excision repair. The data suggest that either low-density lipoprotein(s), or a component(s) of low-density lipoprotein(s), is required by human lymphocytes in order to initiate excision repair of carcinogen-damaged DNA. 相似文献
8.
Summary Kinetic studies of the uptake of hydroquinone--D-glucoside (arbutin) by excised roots of barley demonstrated that this compound is actively transported. Similar studies on the uptake of hydroquinone indicated that the latter compound enters the root tissues by diffusion. A concentration gradient favouring diffusion is maintained for at least three hours by the conversion of the aglucone to its correspondings glucoside. Hydroquinone, at a concentration of 5 mM, reduced uptake of 86rubidium ion by approximately 30%.This work was supported by a grant from the National Research. Council of Canada 相似文献
9.
10.
Ted C. J. Turlings James H. Tumlinson W. Joe Lewis Louise E. M. Vet 《Journal of Insect Behavior》1989,2(2):217-225
Females of Cotesia marginiventris(Cresson), a generalist larval parasitoid, were observed to respond to host related odors in a four-arm olfactometer. The females were significantly more responsive to the odors after a brief contact experience with host-damaged leaves contaminated with host by products. During the experience, actual encounters with hosts were not required to improve subsequent responses to host-related odors. The response to odors of the plant-host complex with which parasitoids had experience was significantly higher than the response to odors of an alternative plant-host complex. This suggests that the experience effect is due, at least partly, to associative learning. We suspect that females of this generalist parasitoid, as was recently found for those of a specialist, recognize specific semiochemicals when they contact frass of suitable host larvae. The parasitoids, subsequently, associate the surrounding odors with the possible presence of hosts, and use these odors as cues in their search for more hosts. This could be an important component in the host-searching behavior of many parasitoids.Mention of a proprietary product does not constitute an endorsement or the recommendation for its use by the USDA. 相似文献