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371.
Production of cattle lacking prion protein 总被引:14,自引:0,他引:14
Richt JA Kasinathan P Hamir AN Castilla J Sathiyaseelan T Vargas F Sathiyaseelan J Wu H Matsushita H Koster J Kato S Ishida I Soto C Robl JM Kuroiwa Y 《Nature biotechnology》2007,25(1):132-138
Prion diseases are caused by propagation of misfolded forms of the normal cellular prion protein PrP(C), such as PrP(BSE) in bovine spongiform encephalopathy (BSE) in cattle and PrP(CJD) in Creutzfeldt-Jakob disease (CJD) in humans. Disruption of PrP(C) expression in mice, a species that does not naturally contract prion diseases, results in no apparent developmental abnormalities. However, the impact of ablating PrP(C) function in natural host species of prion diseases is unknown. Here we report the generation and characterization of PrP(C)-deficient cattle produced by a sequential gene-targeting system. At over 20 months of age, the cattle are clinically, physiologically, histopathologically, immunologically and reproductively normal. Brain tissue homogenates are resistant to prion propagation in vitro as assessed by protein misfolding cyclic amplification. PrP(C)-deficient cattle may be a useful model for prion research and could provide industrial bovine products free of prion proteins. 相似文献
372.
Pedelini L Marquina M Ariño J Casamayor A Sanz L Bollen M Sanz P Garcia-Gimeno MA 《The Journal of biological chemistry》2007,282(5):3282-3292
We have recently characterized Ypi1 as an inhibitory subunit of yeast Glc7 PP1 protein phosphatase. In this work we demonstrate that Ypi1 forms a complex with Glc7 and Sds22, another Glc7 regulatory subunit that targets the phosphatase to substrates involved in cell cycle control. Interestingly, the combination of equimolar amounts of Ypi1 and Sds22 leads to an almost full inhibition of Glc7 activity. Because YPI1 is an essential gene, we have constructed conditional mutants that demonstrate that depletion of Ypi1 leads to alteration of nuclear localization of Glc7 and cell growth arrest in mid-mitosis with aberrant mitotic spindle. These phenotypes mimic those produced upon inactivation of Sds22. The fact that progressive depletion of either Ypi1 or Sds22 resulted in similar physiological phenotypes and that both proteins inhibit the phosphatase activity of Glc7 strongly suggest a common role of these two proteins in regulating Glc7 nuclear localization and function. 相似文献
373.
Funicello M Novelli M Ragni M Vottari T Cocuzza C Soriano-Lopez J Chiellini C Boschi F Marzola P Masiello P Saftig P Santini F St-Jacques R Desmarais S Morin N Mancini J Percival MD Pinchera A Maffei M 《PloS one》2007,2(8):e683
Growing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenotype of ctsk deficient animals (ctsk-/-). When the growth rate of ctsk-/- was compared to that of the wild type animals (WT), we could establish a time window (5-8 weeks of age) within which ctsk-/-display significantly lower body weight and WAT size as compared to WT. Such a difference was not observable in older mice. Upon treatment with high fat diet (HFD) for 12 weeks ctsk-/- gained significantly less weight than WT and showed reduced brown adipose tissue, liver mass and a lower percentage of body fat. Plasma triglycerides, cholesterol and leptin were significantly lower in HFD-fed-ctsk-/- as compared to HFD-fed WT animals. Adipocyte lipolysis rates were increased in both young and HFD-fed-ctsk-/-, as compared to WT. Carnitine palmitoyl transferase-1 activity, was higher in mitochondria isolated from the WAT of HFD treated ctsk-/- as compared to WT. Together, these data indicate that ctsk ablation in mice results in reduced body fat content under conditions requiring a rapid accumulation of fat stores. This observation could be partly explained by an increased release and/or utilization of FFA and by an augmented ratio of lipolysis/lipogenesis. These results also demonstrate that under a HFD, ctsk deficiency confers a partial resistance to the development of dyslipidemia. 相似文献
374.
Hassan Hosseini Monfared Joaquin Sanchiz Zahra Kalantari 《Inorganica chimica acta》2009,362(10):3791-3886
The in-situ formed hydrazone Schiff base ligand (E)-N′-(2-oxy-3-methoxybenzylidene)benzohydrazide (L2−) reacts with copper(II) acetate to a tetranuclear open cubane [Cu(L)]4 complex which crystallizes as two symmetry-independent (Z′ = 2) S4-symmetrical molecules in different twofold special positions with a homodromic water tetramer. The two independent (A and B) open- or pseudo-cubanes with Cu4O4 cores of 4 + 2 class (Ruiz classification) each have three different magnetic exchange pathways leading to an overall antiferromagnetic coupling with J1B = J2B = −17.2 cm−1, J1A = −36.7 cm−1, J2A = −159 cm−1, J3A = J3B = 33.5 cm−1, g = 2.40 and ρ = 0.0687. The magnetic properties have been analysed using the H = −Σi,jJij(SiSj) spin Hamiltonian. 相似文献
375.
The Escherichia coli HtrA protein is a periplasmic protease/chaperone that is upregulated under stress conditions. The protease and chaperone activities of HtrA eliminate or refold damaged and unfolded proteins in the bacterial periplasm that are generated upon stress conditions. In the absence of substrates, HtrA oligomerizes into a hexameric cage, but binding of misfolded proteins transforms the hexamers into bigger 12-mer and 24-mer cages that encapsulate the substrates for degradation or refolding. HtrA also undergoes partial degradation as a consequence of self-cleavage of the mature protein, producing short-HtrA protein (s-HtrA). The aim of this study was to examine the physiological role of this self-cleavage process. We found that the only requirement for self-cleavage of HtrA into s-HtrA in vitro was the hydrolysis of protein substrates. In fact, peptides resulting from the hydrolysis of the protein substrates were sufficient to induce autocleavage. However, the continuous presence of full-length substrate delayed the process. In addition, we observed that the hexameric cage structure is required for autocleavage and that s-HtrA accumulates only late in the degradation reaction. These results suggest that self-cleavage occurs when HtrA reassembles back into the resting hexameric structure and peptides resulting from substrate hydrolysis are allosterically stimulating the HtrA proteolytic activity. Our data support a model in which the physiological role of the self-cleavage process is to eliminate the excess of HtrA once the stress conditions cease.The cell envelope of gram-negative bacteria mediates the communication of the cell with the environment, and it is responsible for many vital functions, including nutrient uptake and interaction with other bacteria and host cells. These activities are performed by a large collection of proteins that make the periplasm a cellular compartment with an even higher protein concentration than the cytoplasm (2). Bacteria are frequently exposed to multiple stresses such as heat shock, osmotic stress, and pH changes and are regularly challenged by the host immune system. Thus, the maintenance of periplasmic proteins in a fully functional state is a challenging task undertaken by the protein quality control system (5). It is generally accepted that under stress conditions misfolded proteins, protein fragments, and mislocalized membrane proteins appear, activating a stress response through three different signal transduction pathways (σE, Cpx, and Bae) (21, 22). Activation of this stress response in the periplasm triggers the upregulation of molecular chaperones, peptidases, proteases, and other enzymes with a role in eliminating or refolding damaged periplasmic proteins.The Escherichia coli HtrA protein (also called DegP or protease Do) is a periplasmic protein (4) that is upregulated under stress conditions such as heat shock (14, 15). HtrA functions as a chaperone and a protease in a temperature-dependent fashion (24). Recent studies have also shown that HtrA substrates targeted for degradation or refolding are recognized differently, suggesting that the mechanisms through which HtrA recognizes the substrate may play a role in the protease-chaperone switch (8).HtrA contains an N-terminal protease domain, followed by the PDZ1 and PDZ2 domains. In the absence of substrates, HtrA oligomerizes into a hexameric cage (12) that represents the resting state of the protein (10, 13). Upon binding to protein substrates, HtrA transforms into bigger cages formed by 12 or 24 monomers that encapsulate substrates for degradation or refolding (9, 13).HtrA is a 474-residue protein whose first 26 amino acids are removed at the N terminus most likely by a signal peptidase rendering the mature 48-kDa protein (14, 15). This form of the protein will hereon be referred to as full-length HtrA. However, it has been described (11, 23) that mature HtrA undergoes partial degradation both in vivo and in vitro as a consequence of self-cleavage occurring after Cys69 and Gln82 of the mature protein. These forms of the protein have been named short-HtrA (s-HtrA) (23).A similar phenomenon of autocleavage has been observed in other members of the HtrA family such as the human homologs HtrA1 (7) and HtrA2 proteins (6). The autocleavage process is not specific for proteases of the HtrA family. Several prokaryotic proteins involved in regulation of gene expression, such as the SOS response proteins LexA (16-18) and UmuD (3), are inactivated through a self-cleaving mechanism. Conversely, many mammalian proteases are produced as longer inactive precursors and depend on an intramolecular cleavage event to become active. This is the case for some gastric proteases such as pepsin and chymosin or the lysosome cathepsins D and E (1).Although autocleavage as a mechanism of activation or inactivation of certain proteases is well documented, the physiological role and the events triggering the self-cleavage of HtrA are poorly understood. In this study, we observed that the hexameric cage structure is required to observe autocleavage of HtrA. In addition, we analyzed the conditions that led to self-cleavage of HtrA and we found that the only requirement to observe accumulation of the s-HtrA form in vitro was the hydrolysis of protein substrates. In fact, peptides resulting from the degradation of protein substrates were sufficient to induce autocleavage. Therefore, considering the current functional model for HtrA (9, 13), our data suggest that the physiological role of the HtrA autocleavage is to eliminate the excess of HtrA protein expressed under stress conditions when the enzymatic activities of the protein are no longer needed. 相似文献
376.
Before we can realize our long term goal of engineering lung tissue worthy of clinical applications, advances in the identification and utilization of cell sources, development of standardized procedures for differentiation of cells, production of matrix tailored to meet the needs of the lung and design of methods or techniques of applying the engineered tissues into the injured lung environment will need to occur. Design of better biomaterials with the capacity to guide stem cell behavior and facilitate lung lineage choice as well as seamlessly integrate with living lung tissue will be achieved through advances in the development of decellularized matrices and new understandings related to the influence of extracellular matrix on cell behavior and function. We have strong hopes that recent developments in the engineering of conducting airway from decellularized trachea will lead to similar breakthroughs in the engineering of distal lung components in the future.Key words: adult and embryonic stem cells, tissue engineered lung, lung stem cells, lung matrices, lung development 相似文献
377.
Tongyu Cao Wikramanayake Elizabeth Alvarez-Connelly Jessica Simon Lucia M. Mauro Javier Guzman George Elgart Lawrence A. Schachner Juan Chen Lisa R. Plano Joaquin J. Jimenez 《Cell stress & chaperones》2010,15(6):985-991
Alopecia areata (AA) is a common autoimmune disease characterized by non-scarring hair loss. Previous studies have demonstrated
an association between AA and physiological/psychological stress. In this study, we investigated the effects of heat treatment,
a physiological stress, on AA development in C3H/HeJ mice. Whereas this strain of mice are predisposed to AA at low incidence
by 18 months of age, we observed a significant increase in the incidence of hair loss in heat-treated 8-month-old C3H/HeJ
mice compared with sham-treated mice. Histological analysis detected mononuclear cell infiltration in anagen hair follicles,
a characteristic of AA, in heat-treated mouse skin. As expected, increased expression of induced HSPA1A/B (formerly called
HSP70i) was detected in skin samples from heat-treated mice. Importantly, increased HSPA1A/B expression was also detected
in skin samples from C3H/HeJ mice that developed AA spontaneously. Our results suggest that induction of HSPA1A/B may precipitate
the development of AA in C3H/HeJ mice. For future studies, the C3H/HeJ mice with heat treatment may prove a useful model to
investigate stress response in AA. 相似文献
378.
Gisela Nogales-Gadea Emma Mormeneo Inés García-Consuegra Juan C. Rubio Anna Orozco Joaquin Arenas Miguel A. Martín Alejandro Lucia Anna M. Gómez-Foix Ramon Martí Antoni L. Andreu 《PloS one》2010,5(10)
Background
Mutations in the PYGM gene encoding skeletal muscle glycogen phosphorylase (GP) cause a metabolic disorder known as McArdle''s disease. Previous studies in muscle biopsies and cultured muscle cells from McArdle patients have shown that PYGM mutations abolish GP activity in skeletal muscle, but that the enzyme activity reappears when muscle cells are in culture. The identification of the GP isoenzyme that accounts for this activity remains controversial.Methodology/Principal Findings
In this study we present two related patients harbouring a novel PYGM mutation, p.R771PfsX33. In the patients'' skeletal muscle biopsies, PYGM mRNA levels were ∼60% lower than those observed in two matched healthy controls; biochemical analysis of a patient muscle biopsy resulted in undetectable GP protein and GP activity. A strong reduction of the PYGM mRNA was observed in cultured muscle cells from patients and controls, as compared to the levels observed in muscle tissue. In cultured cells, PYGM mRNA levels were negligible regardless of the differentiation stage. After a 12 day period of differentiation similar expression of the brain and liver isoforms were observed at the mRNA level in cells from patients and controls. Total GP activity (measured with AMP) was not different either; however, the active GP activity and immunoreactive GP protein levels were lower in patients'' cell cultures. GP immunoreactivity was mainly due to brain and liver GP but muscle GP seemed to be responsible for the differences.Conclusions/Significance
These results indicate that in both patients'' and controls'' cell cultures, unlike in skeletal muscle tissue, most of the protein and GP activities result from the expression of brain GP and liver GP genes, although there is still some activity resulting from the expression of the muscle GP gene. More research is necessary to clarify the differential mechanisms of metabolic adaptations that McArdle cultures undergo in vitro. 相似文献379.
Mari Carmen Gil Borlado David Moreno Lastres Maritza Gonzalez Hoyuela Maria Moran Alberto Blazquez Rosa Pello Lorena Marin Buera Toni Gabaldon Juan Jose Garcia Pe?as Miguel A. Martín Joaquin Arenas Cristina Ugalde 《PloS one》2010,5(9)
Background
In recent years clinical evidence has emphasized the importance of the mtDNA genetic background that hosts a primary pathogenic mutation in the clinical expression of mitochondrial disorders, but little experimental confirmation has been provided. We have analyzed the pathogenic role of a novel homoplasmic mutation (m.15533 A>G) in the cytochrome b (MT-CYB) gene in a patient presenting with lactic acidosis, seizures, mild mental delay, and behaviour abnormalities.Methodology
Spectrophotometric analyses of the respiratory chain enzyme activities were performed in different tissues, the whole muscle mitochondrial DNA of the patient was sequenced, and the novel mutation was confirmed by PCR-RFLP. Transmitochondrial cybrids were constructed to confirm the pathogenicity of the mutation, and assembly/stability studies were carried out in fibroblasts and cybrids by means of mitochondrial translation inhibition in combination with blue native gel electrophoresis.Principal Findings
Biochemical analyses revealed a decrease in respiratory chain complex III activity in patient''s skeletal muscle, and a combined enzyme defect of complexes III and IV in fibroblasts. Mutant transmitochondrial cybrids restored normal enzyme activities and steady-state protein levels, the mutation was mildly conserved along evolution, and the proband''s mother and maternal aunt, both clinically unaffected, also harboured the homoplasmic mutation. These data suggested a nuclear genetic origin of the disease. However, by forcing the de novo functioning of the OXPHOS system, a severe delay in the biogenesis of the respiratory chain complexes was observed in the mutants, which demonstrated a direct functional effect of the mitochondrial genetic background.Conclusions
Our results point to possible pitfalls in the detection of pathogenic mitochondrial mutations, and highlight the role of the genetic mtDNA background in the development of mitochondrial disorders. 相似文献380.
Complex coherent dynamics is present in a wide variety of neural systems. A typical example is the voltage transitions between up and down states observed in cortical areas in the brain. In this work, we study this phenomenon via a biologically motivated stochastic model of up and down transitions. The model is constituted by a simple bistable rate dynamics, where the synaptic current is modulated by short-term synaptic processes which introduce stochasticity and temporal correlations. A complete analysis of our model, both with mean-field approaches and numerical simulations, shows the appearance of complex transitions between high (up) and low (down) neural activity states, driven by the synaptic noise, with permanence times in the up state distributed according to a power-law. We show that the experimentally observed large fluctuation in up and down permanence times can be explained as the result of sufficiently noisy dynamical synapses with sufficiently large recovery times. Static synapses cannot account for this behavior, nor can dynamical synapses in the absence of noise. 相似文献