全文获取类型
收费全文 | 4527篇 |
免费 | 323篇 |
国内免费 | 1篇 |
专业分类
4851篇 |
出版年
2024年 | 3篇 |
2023年 | 33篇 |
2022年 | 77篇 |
2021年 | 127篇 |
2020年 | 52篇 |
2019年 | 83篇 |
2018年 | 113篇 |
2017年 | 133篇 |
2016年 | 173篇 |
2015年 | 230篇 |
2014年 | 276篇 |
2013年 | 333篇 |
2012年 | 417篇 |
2011年 | 426篇 |
2010年 | 230篇 |
2009年 | 170篇 |
2008年 | 249篇 |
2007年 | 272篇 |
2006年 | 254篇 |
2005年 | 267篇 |
2004年 | 226篇 |
2003年 | 192篇 |
2002年 | 172篇 |
2001年 | 52篇 |
2000年 | 19篇 |
1999年 | 28篇 |
1998年 | 44篇 |
1997年 | 19篇 |
1996年 | 17篇 |
1995年 | 21篇 |
1994年 | 10篇 |
1993年 | 15篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 9篇 |
1989年 | 6篇 |
1988年 | 10篇 |
1987年 | 14篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1968年 | 2篇 |
1967年 | 2篇 |
排序方式: 共有4851条查询结果,搜索用时 15 毫秒
31.
Human brain function is mediated by biochemical processes, many of which can be visualized and quantified by positron emission tomography (PET). PET brain imaging of monoamine oxidase A (MAOA)—an enzyme metabolizing neurotransmitters—revealed that MAOA levels vary widely between healthy men and this variability was not explained by the common MAOA genotype (VNTR genotype), suggesting that environmental factors, through epigenetic modifications, may mediate it. Here, we analyzed MAOA methylation in white blood cells (by bisulphite conversion of genomic DNA and subsequent sequencing of cloned DNA products) and measured brain MAOA levels (using PET and [11C]clorgyline, a radiotracer with specificity for MAOA) in 34 healthy non-smoking male volunteers. We found significant interindividual differences in methylation status and methylation patterns of the core MAOA promoter. The VNTR genotype did not influence the methylation status of the gene or brain MAOA activity. In contrast, we found a robust association of the regional and CpG site-specific methylation of the core MAOA promoter with brain MAOA levels. These results suggest that the methylation status of the MAOA promoter (detected in white blood cells) can reliably predict the brain endophenotype. Therefore, the status of MAOA methylation observed in healthy males merits consideration as a variable contributing to interindividual differences in behavior. 相似文献
32.
33.
Yao Shi Juan Yuan Vilma Rraklli Eva Maxymovitz Miriam Cipullo Mingzhi Liu Shuijie Li Isabelle Westerlund Oscar C Bedoya-Reina Petra Bullova Joanna Rorbach C Christofer Juhlin Adam Stenman Catharina Larsson Per Kogner Maureen J OSullivan Susanne Schlisio Johan Holmberg 《Nucleic acids research》2021,49(5):2509
34.
35.
36.
37.
Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis 下载免费PDF全文
Ursula Ferretti Joanna Ciura Brigitte Ksas Marek Rác Michaela Sedlářová Jerzy Kruk Michel Havaux Pavel Pospíšil 《The Plant journal : for cell and molecular biology》2018,95(5):848-861
Prenylquinols (tocochromanols and plastoquinols) serve as efficient physical and chemical quenchers of singlet oxygen (1O2) formed during high light stress in higher plants. Although quenching of 1O2 by prenylquinols has been previously studied, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is limited in vivo. In the present study, the role of plastoquinol‐9 (PQH2‐9) in chemical quenching of 1O2 was studied in Arabidopsis thaliana lines overexpressing the SOLANESYL DIPHOSPHATE SYNTHASE 1 gene (SPS1oex) involved in PQH2‐9 and plastochromanol‐8 biosynthesis. In this work, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is presented, which is obtained by microscopic techniques in vivo. Chemical quenching of 1O2 was associated with consumption of PQH2‐9 and formation of its various oxidized forms. Oxidation of PQH2‐9 by 1O2 leads to plastoquinone‐9 (PQ‐9), which is subsequently oxidized to hydroxyplastoquinone‐9 [PQ(OH)‐9]. We provide here evidence that oxidation of PQ(OH)‐9 by 1O2 results in the formation of trihydroxyplastoquinone‐9 [PQ(OH)3‐9]. It is concluded here that PQH2‐9 serves as an efficient 1O2 chemical quencher in Arabidopsis, and PQ(OH)3‐9 can be considered as a natural product of 1O2 reaction with PQ(OH)‐9. The understanding of the mechanisms underlying 1O2 chemical quenching provides information on the role of plastoquinols and their oxidation products in the response of plants to photooxidative stress. 相似文献
38.
39.
Magdalena Chelchowska Jadwiga Ambroszkiewicz Katarzyna Jablonka-Salach Joanna Gajewska Tomasz M. Maciejewski Ewa Bulska Teresa Laskowska-Klita Jerzy Leibschang 《Biological trace element research》2013,155(2):169-175
To assess the effect of lead exposure from cigarette smoke on fetal growth, blood lead concentrations were measured using inductively coupled plasma mass spectrometry in 150 healthy pregnant women. Mean lead concentrations in plasma and whole blood were significantly higher in the smoking group compared with the nonsmoking group in each trimester of pregnancy (p?<?0.001). Logistic regression analysis showed the highest impact of the number of cigarettes smoked per day for serum lead concentration (β?=?0.238; p?<?0.05), while in whole blood, it was duration of smoking before conception (β?=?0.297; p?<?0.001). Birth weight of the smoking mothers' infants was significantly lower (mean?±?SEM, 3,192?±?50.8 and 3,569?±?49.6 g, respectively; p?<?0.001) and negatively correlated with lead levels in plasma (r?=??0.38; p?<?0.001) and in whole blood (r?=??0.27; p?<?0.001). Therefore, it is suggested that smoking during pregnancy increases lead concentrations in maternal blood. Fetal exposure to low doses of lead in utero may be a serious risk factor causing lower birth weight. 相似文献
40.
Bioluminescence imaging is widely used for optical cell tracking approaches. However, reliable and quantitative bioluminescence of transplanted cells in the brain is highly challenging. In this study we established a new bioluminescence imaging protocol dedicated for neuroimaging, which increases sensitivity especially for noninvasive tracking of brain cell grafts. Different D-Luciferin concentrations (15, 150, 300 and 750 mg/kg), injection routes (iv, ip, sc), types of anesthesia (Isoflurane, Ketamine/Xylazine, Pentobarbital) and timing of injection were compared using DCX-Luc transgenic mice for brain specific bioluminescence. Luciferase kinetics was quantitatively evaluated for maximal photon emission, total photon emission and time-to-peak. Photon emission followed a D-Luciferin dose-dependent relation without saturation, but with delay in time-to-peak increasing for increasing concentrations. The comparison of intravenous, subcutaneous and intraperitoneal substrate injection reflects expected pharmacokinetics with fastest and highest photon emission for intravenous administration. Ketamine/Xylazine and Pentobarbital anesthesia showed no significant beneficial effect on maximal photon emission. However, a strong difference in outcome was observed by injecting the substrate pre Isoflurane anesthesia. This protocol optimization for brain specific bioluminescence imaging comprises injection of 300 mg/kg D-Luciferin pre Isoflurane anesthesia as an efficient and stable method with a signal gain of approx. 200% (compared to 150 mg/kg post Isoflurane). Gain in sensitivity by the novel imaging protocol was quantitatively assessed by signal-to-noise calculations of luciferase-expressing neural stem cells grafted into mouse brains (transplantation of 3,000–300,000 cells). The optimized imaging protocol lowered the detection limit from 6,000 to 3,000 cells by a gain in signal-to-noise ratio. 相似文献