全文获取类型
收费全文 | 4576篇 |
免费 | 359篇 |
国内免费 | 4篇 |
专业分类
4939篇 |
出版年
2022年 | 25篇 |
2021年 | 55篇 |
2020年 | 31篇 |
2019年 | 49篇 |
2018年 | 49篇 |
2017年 | 56篇 |
2016年 | 105篇 |
2015年 | 164篇 |
2014年 | 200篇 |
2013年 | 279篇 |
2012年 | 333篇 |
2011年 | 314篇 |
2010年 | 180篇 |
2009年 | 188篇 |
2008年 | 274篇 |
2007年 | 293篇 |
2006年 | 264篇 |
2005年 | 270篇 |
2004年 | 260篇 |
2003年 | 243篇 |
2002年 | 227篇 |
2001年 | 49篇 |
2000年 | 40篇 |
1999年 | 58篇 |
1998年 | 65篇 |
1997年 | 39篇 |
1996年 | 43篇 |
1995年 | 44篇 |
1994年 | 34篇 |
1993年 | 36篇 |
1992年 | 54篇 |
1991年 | 38篇 |
1990年 | 40篇 |
1989年 | 25篇 |
1988年 | 33篇 |
1987年 | 31篇 |
1986年 | 22篇 |
1985年 | 30篇 |
1984年 | 41篇 |
1983年 | 20篇 |
1982年 | 38篇 |
1981年 | 21篇 |
1980年 | 11篇 |
1979年 | 19篇 |
1978年 | 19篇 |
1977年 | 16篇 |
1976年 | 19篇 |
1975年 | 19篇 |
1974年 | 13篇 |
1969年 | 10篇 |
排序方式: 共有4939条查询结果,搜索用时 15 毫秒
81.
Elisabeth Breitenlechner Gert Goldenberg Joachim Lutz Klaus Oeggl 《Vegetation History and Archaeobotany》2013,22(4):351-366
The exploitation of copper ore deposits of the northern Greywacke Zone was initiated by the implementation of metallurgic technologies in the Eastern Alps thousands of years ago. This multi-proxy study aimed to detect prehistoric mining phases in the vicinity of a prominent copper ore deposit in the Lower Inn Valley. Therefore we studied a peat core from a fen using pollen, micro charcoal and geochemical analyses. In the same fen, an archaeological investigation revealed an ore beneficiation site, well dated by dendrochronological analysis to the Late Bronze Age (9th century b.c.). First hints of mining activities reflected by the occurrence of anthropogenic indicators in the pollen diagram, associated with elevated metal values, at the beginning of the Bronze Age might result from early mineral prospecting and metallurgical experiments around the use of fahlore. The local ore deposit was then abandoned until during the Bronze Age mining activities started to increase. This is reflected by an expansion of the pioneer species Pinus and Larix on mine spoil heaps in the proximity. Concomitantly metal ratios and micro charcoal increase. From about 1000 to 850 b.c. a strong impact of mining activities is displayed in the multi-proxy data. The local forest was partly cleared on and in the vicinity of the fen. According to dendrochronological data the ore beneficiation plant was in use from about 900 to 870 b.c. Until about 700 b.c. another period with moderate impact by mining activities in the further vicinity of the fen shows up. 相似文献
82.
83.
84.
85.
86.
Stefan Thiem Moritz F. Eissmann Emma Stuart Joachim Elzer Anna Jonas Michael Buchert Matthias Ernst 《Genesis (New York, N.Y. : 2000)》2016,54(12):626-635
Temporal and spatial regulation of genes mediated by tissue‐specific promoters and conditional gene expression systems provide a powerful tool to study gene function in health, disease, and during development. Although transgenic mice expressing the Cre recombinase in the gastric epithelium have been reported, there is a lack of models that allow inducible and reversible gene modification in the stomach. Here, we exploited the gastrointestinal epithelium‐specific expression pattern of the three trefoil factor (Tff) genes and bacterial artificial chromosome transgenesis to generate a novel mouse strain that expresses the CreERT2 recombinase and the reverse tetracycline transactivator (rtTA). The Tg(Tff1‐CreERT2;Tff2‐rtTA;Tff3‐Luc) strain confers tamoxifen‐inducible irreversible somatic recombination and allows simultaneous doxycycline‐dependent reversible gene activation in the gastric epithelium of developing and adult mice. This strain also confers luciferase activity to the intestinal epithelium to enable in vivo bioluminescence imaging. Using fluorescent reporters as conditional alleles, we show Tff1‐CreERT2 and Tff2‐rtTA transgene activity in a partially overlapping subset of long‐term regenerating gastric stem/progenitor cells. Therefore, the Tg(Tff1‐CreERT2;Tff2‐rtTA;Tff3‐Luc) strain can confer intermittent transgene expression to gastric epithelial cells that have undergone previous gene modification, and may be suitable to genetically model therapeutic intervention during development, tumorigenesis, and other genetically tractable diseases. Birth Defects Research (Part A) 106:626–635, 2016. © 2016 Wiley Periodicals, Inc. 相似文献
87.
88.
Sylvia Hofmann Chitra B. Baniya Spartak N. Litvinchuk Georg Miehe Jia‐Tang Li Joachim Schmidt 《Ecology and evolution》2019,9(24):14498-14511
Recent advances in the understanding of the evolution of the Asian continent challenge the long‐held belief of a faunal immigration into the Himalaya. Spiny frogs of the genus Nanorana are a characteristic faunal group of the Himalaya–Tibet orogen (HTO). We examine the phylogeny of these frogs to explore alternative biogeographic scenarios for their origin in the Greater Himalaya, namely, immigration, South Tibetan origin, strict vicariance. We sequenced 150 Nanorana samples from 62 localities for three mitochondrial (1,524 bp) and three nuclear markers (2,043 bp) and complemented the data with sequence data available from GenBank. We reconstructed a gene tree, phylogenetic networks, and ancestral areas. Based on the nuDNA, we also generated a time‐calibrated species tree. The results revealed two major clades (Nanorana and Quasipaa), which originated in the Lower Miocene from eastern China and subsequently spread into the HTO (Nanorana). Five well‐supported subclades are found within Nanorana: from the East, Central, and Northwest Himalaya, the Tibetan Plateau, and the southeastern Plateau margin. The latter subclade represents the most basal group (subgenus Chaparana), the Plateau group (Nanorana) represents the sister clade to all species of the Greater Himalaya (Paa). We found no evidence for an east–west range expansion of Paa along the Himalaya, nor clear support for a strict vicariance model. Diversification in each of the three Himalayan subclades has probably occurred in distinct areas. Specimens from the NW Himalaya are placed basally relative to the highly diverse Central Himalayan group, while the lineage from the Tibetan Plateau is placed within a more terminal clade. Our data indicate a Tibetan origin of Himalayan Nanorana and support a previous hypothesis, which implies that a significant part of the Himalayan biodiversity results from primary diversification of the species groups in South Tibet before this part of the HTO was uplifted to its recent heights. 相似文献
89.
90.
We investigate adaptive evolution of a quantitative trait under stabilizing selection with a moving optimum. We characterize three regimes, depending on whether (1) the beneficial mutation rate, (2) the fixation time, or (3) the rate of environmental change is the limiting factor for adaptation. If the environment is rate limiting, mutations with a small phenotypic effect are preferred over large mutations, in contrast to standard theory. 相似文献