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41.
叶下珠有效部位对血栓形成的影响及其作用机制初探 总被引:6,自引:1,他引:6
采用Born方法和改良的Hamburger方法分别测定叶下珠 (Phyllanthusurinaria)含corilagin的有效部位 (代号PUW )在体内外对血小板聚集功能和对血小板与中性粒细胞之间粘附反应的影响 ;应用Myers方法评价PUW灌胃对小鼠尾静脉注射花生四烯酸 (AA)引起猝死的保护作用 ;运用改良的Charl ton方法及陈长勋等方法分别观察PUW灌胃对电刺激大鼠颈动脉血栓形成和下腔静脉血栓形成的影响 ;采用Tomihisa方法 ,观察PUW对大鼠尾尖出血时间的影响。结果显示 ,PUW在体内外对ADP、AA或血小板活化因子 (PAF)诱导的血小板聚集均无明显抑制作用 ;PUW呈浓度依赖性明显阻抑AA激活的血小板与中性粒细胞之间的粘附反应 ,其半数抑制浓度 (IC50 )为 39 7mg/kg。PUW (10、2 0和 4 0mg/kg)灌胃呈剂量依赖性显著减少AA致小鼠死亡的数量 ,明显延长电刺激大鼠颈动脉血栓形成时间 ,减轻大鼠下腔静脉血栓的干、湿重。 2 0mg/kg的PUW对出血时间无明显影响 ,4 0mg/kg的PUW虽延长出血时间 ,但与阿司匹林 (2 0mg/kg)比较 ,出血时间明显缩短 (P <0 0 5 )。本实验结果提示 ,PUW灌胃在多种体内血栓模型中均具有明显的抗血栓形成作用 ,其机制可能与阻抑血小板和中性粒细胞之间的的粘附作用密切相关。 相似文献
42.
雄性麋鹿的吼叫行为、序位等级与成功繁殖 总被引:14,自引:1,他引:14
为探讨雄性麋鹿(Elaphurus davidianus)的吼叫行为与序位等级的关系,以及这种关系在成功繁殖中的作用。2000年7-8月,在北京麋鹿苑选定12头成体雄鹿,用目标取样法观察记录每头鹿的吼叫、圈群和交配行为的发生频次。用有效比值[被雌鹿接受的频次/雄鹿圈群(或交配)的总频次]来衡量雄鹿圈群行为(或交配行为)的有效性。依据雄鹿在发情期的表现状态,把雄鹿归为3类:占有雌鹿群的“群主”(4头)、未占有雌鹿群而挑战“群主”的“挑战者”(5头)和远离繁殖群的“单身汉”(3头)。不同序位等级雄鹿的吼叫频次、圈群(或交配)行为频次、圈群行为(或交配行为)的有效值存在显著差异(P<0.05)。对吼叫行为的分析表明:“群主”吼叫频次最高,“单身汉”吼叫频次最低。“群主”的有效值最高,“单身汉”的有效值最低。据此认为雄性麋鹿的吼叫行为与其序位等级密切相关,而序位等级又决定了雄鹿参与繁殖的机会。此外,还记录到占群雄鹿的警戒吼叫,这是对Wemmer et al.(1983)警戒吼叫行为仅见于雌鹿的观察结果的补充。 相似文献
43.
为了探讨CRF在抑郁症发生发展过程中的作用.对正常大鼠侧脑室慢性注射CRF21天并与慢性非预见性应激刺激21天建立的抑郁症模型大鼠进行比较。运用旷场行为实验(open-field)观察大鼠主动性活动能力.用Morris water Maze法.以训练期的逃避潜伏期为指标检测大鼠空间学习记忆能力。采用HPLC—UV法测定血清皮质醇含量,RT—PCR法检测CRF及其受体mRNA的表达。结果显示:慢性应激21天建立的模型大鼠主动性活动和学习记忆能力均明显下降.血清皮质醇含量显著升高,CRF及其受体R1 mRNA的表达增加。大鼠侧脑室慢性注射CRF21天后.其体重增量、主动性活动和学习记忆能力与慢性应激模型大鼠一样均明显降低。这些工作证明了CRF在抑郁症的发生发展过程中发挥了至关重要的作用.慢性应激导致机体CRF分泌持续增加可能是抑郁症发病的主要原因。 相似文献
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46.
虎纹捕鸟蛛的饲养与繁殖技术 总被引:12,自引:0,他引:12
本文简要介绍饲养、繁殖虎纹捕鸟蛛的技术,包括它的形态特征、生活习性、食物种类及饲养器具等,并介绍了在饲养和繁殖中应注意的一些问题。 相似文献
47.
Sun QiangLing Liu YinKun Lu WenJing Cheng Gang Zhou HaiJun Zhou XinWen Wei LiMing Dai Zhi Guo Kun Lu HaoJie 《中国科学C辑(英文版)》2007,50(3):403-411
In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins
with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified
and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue
were generated by using SDS-PAGE, two-dimensional electrophoresis (2-DE) followed by fast fluorescence staining based on multiplexed
proteomics (MP) technology. 301 visible protein spots on the gel were detected and 85 of glycoproteins were further successfully
identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF-MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell
cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile
they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal
human liver tissue would contribute to the subsequent research. 相似文献
48.
Xin‐Hua Ma Zhi‐Biao Wang Lei Zhang Wei Li Cui‐Min Deng Tian‐Hua Zhong Guang‐Yu Li Wei‐Ming Zheng Yong‐Hong Zhang 《化学与生物多样性》2017,14(5)
One new ent‐kaurane diterpenoid, 11β,16α‐dihydroxy‐ent‐kauran‐19‐oic acid ( 1 ), together with eight known analogues 2 – 9 were isolated from the aerial parts of Wedelia prostrata. One of the acidic diterpenoids, kaurenoic acid ( 3 ), was converted to seven derivatives, 10 – 16 . All compounds were evaluated for their cytotoxic activity in vitro against human leukemia (K562), liver (HepG‐2), and stomach (SGC‐7901) cancer cell lines. Only four kaurenoic acid derivatives, 13 – 16 , with 15‐keto and substitutions at C(19) position, exhibited notable cytotoxic activities on these tumor cell lines with IC50 value ranging from 0.05 to 3.71 μm . Compounds 10 – 12 , with oxime on C(15) showed moderate inhibitory effects and compounds 1 – 9 showed no cytotoxicities on them. Structure–activity relationships were also discussed based on the experimental data obtained. The known derivative, 15‐oxokaurenoic acid 4‐piperdin‐1‐ylbutyl ester ( 17 ), induced typical apoptotic cell death in colon SW480 cells upon evaluation of the apoptosis‐inducing activity by flow‐cytometric analysis. 相似文献
49.
Ge X McFarlane C Vajjala A Lokireddy S Ng ZH Tan CK Tan NS Wahli W Sharma M Kambadur R 《Cell research》2011,21(11):1591-1604
TGF-β and myostatin are the two most important regulators of muscle growth. Both growth factors have been shown to signal through a Smad3-dependent pathway. However to date, the role of Smad3 in muscle growth and differentiation is not investigated. Here, we demonstrate that Smad3-null mice have decreased muscle mass and pronounced skeletal muscle atrophy. Consistent with this, we also find increased protein ubiquitination and elevated levels of the ubiquitin E3 ligase MuRF1 in muscle tissue isolated from Smad3-null mice. Loss of Smad3 also led to defective satellite cell (SC) functionality. Smad3-null SCs showed reduced propensity for self-renewal, which may lead to a progressive loss of SC number. Indeed, decreased SC number was observed in skeletal muscle from Smad3-null mice showing signs of severe muscle wasting. Further in vitro analysis of primary myoblast cultures identified that Smad3-null myoblasts exhibit impaired proliferation, differentiation and fusion, resulting in the formation of atrophied myotubes. A search for the molecular mechanism revealed that loss of Smad3 results in increased myostatin expression in Smad3-null muscle and myoblasts. Given that myostatin is a negative regulator, we hypothesize that increased myostatin levels are responsible for the atrophic phenotype in Smad3-null mice. Consistent with this theory, inactivation of myostatin in Smad3-null mice rescues the muscle atrophy phenotype. 相似文献
50.