全文获取类型
收费全文 | 4919篇 |
免费 | 452篇 |
国内免费 | 350篇 |
出版年
2024年 | 15篇 |
2023年 | 74篇 |
2022年 | 163篇 |
2021年 | 260篇 |
2020年 | 175篇 |
2019年 | 259篇 |
2018年 | 221篇 |
2017年 | 137篇 |
2016年 | 244篇 |
2015年 | 359篇 |
2014年 | 348篇 |
2013年 | 371篇 |
2012年 | 444篇 |
2011年 | 368篇 |
2010年 | 267篇 |
2009年 | 239篇 |
2008年 | 259篇 |
2007年 | 238篇 |
2006年 | 199篇 |
2005年 | 142篇 |
2004年 | 132篇 |
2003年 | 130篇 |
2002年 | 98篇 |
2001年 | 91篇 |
2000年 | 58篇 |
1999年 | 73篇 |
1998年 | 46篇 |
1997年 | 36篇 |
1996年 | 41篇 |
1995年 | 28篇 |
1994年 | 32篇 |
1993年 | 31篇 |
1992年 | 31篇 |
1991年 | 27篇 |
1990年 | 23篇 |
1989年 | 11篇 |
1988年 | 16篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1985年 | 11篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有5721条查询结果,搜索用时 15 毫秒
61.
62.
D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA. 总被引:114,自引:0,他引:114
Human cyclin D1 has been associated with a wide variety of proliferative diseases but its biochemical role is unknown. In diploid fibroblasts we find that cyclin D1 is complexed with many other cellular proteins. Among them are protein kinase catalytic subunits CDK2, CDK4 (previously called PSK-J3), and CDK5 (also called PSSALRE). In addition, polypeptides of 21 kd and 36 kd are identified in association with cyclin D1. We show that the 36 kd protein is the proliferating cell nuclear antigen, PCNA. Cyclin D3 also associates with multiple protein kinases, p21 and PCNA. It is proposed that there exists a quaternary complex of D cyclin, CDK, PCNA, and p21 and that many combinatorial variations (cyclin D1, D3, CDK2, 4, and 5) may assemble in vivo. These findings link a human putative G1 cyclin that is associated with oncogenesis with a well-characterized DNA replication and repair factor. 相似文献
63.
64.
Plant Ecology - There is increasing evidence that climate change and nutrient fluctuations can affect the invasion of alien plants. However, most studies have been performed in pairwise experiments... 相似文献
65.
66.
Yuan Xiong Chenchen Yan Lang Chen Yori Endo Yun Sun Wu Zhou Yiqiang Hu Liangcong Hu Dong Chen Hang Xue Bobin Mi Guohui Liu 《Journal of cellular and molecular medicine》2020,24(1):1076-1086
Interleukin‐10 (IL‐10) displays well‐documented anti‐inflammatory effects, but its effects on osteoblast differentiation have not been investigated. In this study, we found IL‐10 negatively regulates microRNA‐7025‐5p (miR‐7025‐5p), the down‐regulation of which enhances osteoblast differentiation. Furthermore, through luciferase reporter assays, we found evidence that insulin‐like growth factor 1 receptor (IGF1R) is a miR‐7025‐5p target gene that positively regulates osteoblast differentiation. In vivo studies indicated that the pre‐injection of IL‐10 leads to increased bone formation, while agomiR‐7025‐5p injection delays fracture healing. Taken together, these results indicate that IL‐10 induces osteoblast differentiation via regulation of the miR‐7025‐5p/IGF1R axis. IL‐10 therefore represents a promising therapeutic strategy to promote fracture healing. 相似文献
67.
Yaoyi Xiong Lushun Yuan Jing Xiong Huimin Xu Yongwen Luo Gang Wang Lingao Ju Yu Xiao Xinghuan Wang 《Journal of cellular and molecular medicine》2020,24(3):2342-2355
The precision evaluation of prognosis is crucial for clinical treatment decision of bladder cancer (BCa). Therefore, establishing an effective prognostic model for BCa has significant clinical implications. We performed WGCNA and DEG screening to initially identify the candidate genes. The candidate genes were applied to construct a LASSO Cox regression analysis model. The effectiveness and accuracy of the prognostic model were tested by internal/external validation and pan‐cancer validation and time‐dependent ROC. Additionally, a nomogram based on the parameter selected from univariate and multivariate cox regression analysis was constructed. Eight genes were eventually screened out as progression‐related differentially expressed candidates in BCa. LASSO Cox regression analysis identified 3 genes to build up the outcome model in E‐MTAB‐4321 and the outcome model had good performance in predicting patient progress free survival of BCa patients in discovery and test set. Subsequently, another three datasets also have a good predictive value for BCa patients' OS and DFS. Time‐dependent ROC indicated an ideal predictive accuracy of the outcome model. Meanwhile, the nomogram showed a good performance and clinical utility. In addition, the prognostic model also exhibits good performance in pan‐cancer patients. Our outcome model was the first prognosis model for human bladder cancer progression prediction via integrative bioinformatics analysis, which may aid in clinical decision‐making. 相似文献
68.
69.
Jun Bian Zhenjian Zhuo Jinhong Zhu Zhonghua Yang Zhang Jiao Yong Li Jiwen Cheng Haixia Zhou Suhong Li Li Li Jing He Yanfei Liu 《Journal of cellular and molecular medicine》2020,24(16):9280-9286
Neuroblastoma ranks as the most commonly seen and deadly solid tumour in infancy. The aberrant activity of m6A‐RNA methyltransferase METTL3 is involved in human cancers. Therefore, functional genetic variants in the METTL3 gene may contribute to neuroblastoma risk. In the current nine‐centre case‐control study, we aimed to analyse the association between the METTL3 gene single nucleotide polymorphisms (SNPs) and neuroblastoma susceptibility. We genotyped four METTL3 gene SNPs (rs1061026 T>G, rs1061027 C>A, rs1139130 A>G, and rs1263801 G>C) in 968 neuroblastoma patients and 1814 controls in China. We found significant associations between these SNPs and neuroblastoma risk in neither single‐locus nor combined analyses. Interestingly, in the stratified analysis, we observed a significant risk association with rs1061027 AA in subgroups of children ≤ 18 months of age (adjusted OR = 1.87, 95% CI = 1.03‐3.41, P = .040) and females (adjusted OR = 1.86, 95% CI = 1.07‐3.24, P = .028). Overall, we identified a significant association between METTL3 gene rs1061027 C>A polymorphism and neuroblastoma risk in children ≤18 months of age and females. Our findings provide novel insights into the genetic determinants of neuroblastoma. 相似文献
70.
Bobin Mi Yuan Xiong Chenchen Yan Lang Chen Hang Xue Adriana C. Panayi Liangcong Hu Yiqiang Hu Wu Zhou Faqi Cao Guohui Liu 《Journal of cellular and molecular medicine》2020,24(11):6385-6396
N6-methyladenosine (m6A) modification has been reported in various diseases and implicated in increasing numbers of biological processes. However, previous studies have not focused on the role of m6A modification in fracture healing. Here, we demonstrated that m6A modifications are decreased during fracture healing and that methyltransferase-like 3 (METTL3) is the main factor involved in the abnormal changes in m6A modifications. Down-regulation of METTL3 promotes osteogenic processes both in vitro and in vivo, and this effect is recapitulated by the suppression of miR-7212-5p maturation. Further studies have shown that miR-7212-5p inhibits osteoblast differentiation in MC3T3-E1 cells by targeting FGFR3. The present study demonstrated an important role of the METTL3/miR-7212-5p/FGFR3 axis and provided new insights on m6A modification in fracture healing. 相似文献