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181.
MiR‐616‐3p modulates cell proliferation and migration through targeting tissue factor pathway inhibitor 2 in preeclampsia 下载免费PDF全文
Yetao Xu Dan Wu Ziyan Jiang Yuanyuan Zhang Sailan Wang Zhonghua Ma Bingqing Hui Jing Wang Weiping Qian Zhiping Ge Lizhou Sun 《Cell proliferation》2018,51(5)
Objectives
Despite improvements in diagnosis and treatment, preeclampsia (PE) continues to pose a significant risk of maternal and foetal morbidity and mortality if not addressed promptly. An increasing number of studies have suggested that tissue factor pathway inhibitor 2 (TFPI2) acts as a suppressor gene, possibly inhibiting multiple serine proteases affecting cell proliferation and migration. It plays an essential role in the occurrence and development of PE, but the pathogenesis remains unclear.Materials and methods
In our research, we performed western blotting, immunohistochemistry and qPCR assays to investigate TFPI2 and miR‐616‐3p expression in preeclamptic placental tissues. Cell assays were performed in HTR‐8/SVneo and JEG3 cell lines. Cell proliferation and migration events were investigated by MTT, EdU and transwell assays. In conjunction with bioinformatics analysis, luciferase reporter assays were performed to elucidate the mechanism by which miR‐616‐3p binds to TFPI2 mRNA.Results
We established that TFPI2 protein levels were significantly upregulated in PE placental tissues. In addition, we found that miR‐616‐3p binds specifically to the 3′‐UTR region of TFPI2 mRNA. Furthermore, miR‐616‐3p knockdown or TFPI2 overexpression substantially impaired cell growth and migration, whereas miR‐616‐3p upregulation or TFPI2 knockdown stimulated cell proliferation and migration. This miR‐616‐3p / TFPI2 axis was also found to affect the epithelial‐mesenchymal transition process in PE.Conclusions
Our results demonstrated that TFPI2 plays a vital role in the progression of PE and might provide a prospective therapeutic strategy to mitigate the severity of the disorder.182.
Acupuncture has been an effective treatment for various pain in China for several thousand years. However, the mechanisms underlying this mysterious ancient healing are still largely unknown. Here we applied photoacoustic microscopy (PAM) to investigate brain hemodynamic changes in response to electronic acupuncture (EA) at ST36 (Zusanli). Due to the high optical absorption of blood at 532 nm, PAM could sensitively probe changes in hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) of cortical regions in high resolution. Six healthy mice were stimulated at the acupoint and three healthy mice were stimulated at sham points. Remarkable CBV changes in sensorimotor and retrosplenial agranular cortex were observed. Results showed the potential of PAM as a visualization tool to study the acupuncture effect on brain hemodynamics in animal models.
183.
错配修复(mismatch repair,MMR)是DNA复制后的一种修复机制,对维持基因组稳定起重要作用.错配修复基因功能缺陷是继癌基因激活、抑癌基因失活之后又一肿瘤的发生、发展机制,错配修复基因的异常表达与全身多种肿瘤相关.涎腺肿瘤为口腔颌面部常见肿瘤之一,具有与其他系统肿瘤相似的组织学类型,多来源于肌上皮.近年来,有关涎腺肿瘤与错配修复基因的关系正逐步成为研究热点,本文就错配修复基因的组成、作用机制以及与涎腺肿瘤发生、发展的关系作一综述. 相似文献
184.
将1mm厚凝固于复印膜上的水琼脂(15%~2.0%)凝胶板侵入含12mmol/L植酸钠的Gly—HCl缓冲液中达1h以上,取出干至胶表面无水迹,于上加Aspergillussp.59—2植酸酶或与电泳后的凝胶板紧贴10~60min。然后水平置于恒温水浴锅中反应一定时间,取出浸入1mol/LH2SO4-2%(NH4)6Mn7O24-10%FeSO4相似文献
185.
Besides mediating the viral entry process, the human immunodeficiency virus (HIV-1) envelope protein gp41 can bind to many host cell components and regulate cell functions. Using a yeast two-hybrid system, we screened a human bone marrow cDNA library and identified a novel gp41-binding protein, CD74 (the MHC class II-associated invariant chain). Here, we report possible biological effects mediated by interaction between gp41 and CD74. We found that HIV-1 gp41 could bind directly to host CD74 in HIV-1-infected cells, and the peptide 6358 derived from gp41 loop region (aa 597-611) could effectively block the gp41-CD74 interaction. As a result of this binding, recombinant soluble gp41 and gp41 peptide 6358 activated the CD74-mediated ERK/MAPK pathway and significantly enhanced HIV-1 infection in vitro. Conversely, the enhancing effect could be suppressed by the recombinant CD74 extracellular domain. These results reveal a novel mechanism underlying gp41 mediation of HIV-1 infection and replication. 相似文献
186.
为了研究ApCl基因的功能,应用基因重组技术将ApCl基因片段克隆入大肠埃希菌—酵母穿梭质拉pGAPZαA,构建重组真核表达质拉pGAPZαA-ApCl,电转化巴斯德单赤酵母GS115,Zeocin筛选出阳性克隆转化子.通过比较转化空载体pGAPZaA和重组载体pGAPZαA-ApCl的不同菌株分别在含有高盐和高山梨醇浓度的液体培养基中生长情况,发现毕赤酵母GS115在转化ApCl基因后其抗旱、抗盐能力显著提高(约3倍),进一步验证了ApCl基因对提高生物抗逆能力有显著作用,为将来分离该蛋白及进一步研究奠定基础. 相似文献
187.
应用单向SDS—PAGE结合蛋白质铬银染色技术对水稻野败型细胞质雄性不育系珍汕97A和其保持系的叶绿体、线粒体和细胞质的蛋白质多肽进行了比较研究,发现两系之间存在明显的差异,生殖器官(穗子)上的差异比营养器官(叶片)上的差异更为显著。在成熟穗上,叶绿体可溶性蛋白不育系有25条带,保持系仅16条带,两者间有19个多肽不同;线粒体可溶性蛋白不育系有28条带,保持系比不育系少30.1和21.8KD两个多肽;细胞质可溶性蛋白丙酮沉淀物的水溶性蛋白组分不育系有24条带,保持系为29条带,两系间却有7条多肽存在差别;细胞质可溶性蛋白丙酮沉淀物的SDS-增溶性蛋白组分不育系有18条带,保持系只有11条带,两者间亦有7条多肽出现差异。由此可以看出,水稻野败型CMS表型的表达可能需要较多个基因的启动和关闭,既与叶绿体和线粒体有关,还涉及到核基因组的作用。 相似文献
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