全文获取类型
收费全文 | 383篇 |
免费 | 23篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 12篇 |
2020年 | 10篇 |
2019年 | 15篇 |
2018年 | 20篇 |
2017年 | 13篇 |
2016年 | 11篇 |
2015年 | 23篇 |
2014年 | 26篇 |
2013年 | 35篇 |
2012年 | 41篇 |
2011年 | 33篇 |
2010年 | 25篇 |
2009年 | 16篇 |
2008年 | 17篇 |
2007年 | 16篇 |
2006年 | 24篇 |
2005年 | 6篇 |
2004年 | 13篇 |
2003年 | 3篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1978年 | 1篇 |
排序方式: 共有406条查询结果,搜索用时 287 毫秒
81.
Genome Sequence of Acinetobacter sp. Strain HA, Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera 总被引:1,自引:0,他引:1
J Malhotra A Dua A Saxena N Sangwan U Mukherjee N Pandey R Rajagopal P Khurana JP Khurana R Lal 《Journal of bacteriology》2012,194(18):5156
In this study, Acinetobacter sp. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera. Here, we report the draft genome sequence (3,125,085 bp) of this strain that consists of 102 contigs, 2,911 predicted coding sequences, and a G+C content of 41%. 相似文献
82.
Nagendra K. Singh Deepak K. Gupta Pawan K. Jayaswal Ajay K. Mahato Sutapa Dutta Sangeeta Singh Shefali Bhutani Vivek Dogra Bikram P. Singh Giriraj Kumawat Jitendra K. Pal Awadhesh Pandit Archana Singh Hukum Rawal Akhilesh Kumar G. Rama Prashat Ambika Khare Rekha Yadav Ranjit S. Raje Mahendra N. Singh Subhojit Datta Bashasab Fakrudin Keshav B. Wanjari Rekha Kansal Prasanta K. Dash Pradeep K. Jain Ramcharan Bhattacharya Kishor Gaikwad Trilochan Mohapatra R. Srinivasan Tilak R. Sharma 《Journal of plant biochemistry and biotechnology.》2012,21(1):98-112
Pigeonpea (Cajanus cajan) is an important grain legume of the Indian subcontinent, South-East Asia and East Africa. More than eighty five percent of the world pigeonpea is produced and consumed in India where it is a key crop for food and nutritional security of the people. Here we present the first draft of the genome sequence of a popular pigeonpea variety ??Asha??. The genome was assembled using long sequence reads of 454 GS-FLX sequencing chemistry with mean read lengths of >550?bp and >10-fold genome coverage, resulting in 510,809,477?bp of high quality sequence. Total 47,004 protein coding genes and 12,511 transposable elements related genes were predicted. We identified 1,213 disease resistance/defense response genes and 152 abiotic stress tolerance genes in the pigeonpea genome that make it a hardy crop. In comparison to soybean, pigeonpea has relatively fewer number of genes for lipid biosynthesis and larger number of genes for cellulose synthesis. The sequence contigs were arranged in to 59,681 scaffolds, which were anchored to eleven chromosomes of pigeonpea with 347 genic-SNP markers of an intra-species reference genetic map. Eleven pigeonpea chromosomes showed low but significant synteny with the twenty chromosomes of soybean. The genome sequence was used to identify large number of hypervariable ??Arhar?? simple sequence repeat (HASSR) markers, 437 of which were experimentally validated for PCR amplification and high rate of polymorphism among pigeonpea varieties. These markers will be useful for fingerprinting and diversity analysis of pigeonpea germplasm and molecular breeding applications. This is the first plant genome sequence completed entirely through a network of Indian institutions led by the Indian Council of Agricultural Research and provides a valuable resource for the pigeonpea variety improvement. 相似文献
83.
Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR 总被引:13,自引:0,他引:13
Jain M Nijhawan A Tyagi AK Khurana JP 《Biochemical and biophysical research communications》2006,345(2):646-651
For accurate and reliable gene expression results, normalization of real-time PCR data is required against a control gene, which displays highly uniform expression in living organisms during various phases of development and under different environmental conditions. We assessed the gene expression of 10 frequently used housekeeping genes, including 18S rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-1alpha, eIF-4a, and beta-TUB, in a diverse set of 25 rice samples. Their expression varied considerably in different tissue samples analyzed. The expression of UBQ5 and eEF-1alpha was most stable across all the tissue samples examined. However, 18S and 25S rRNA exhibited most stable expression in plants grown under various environmental conditions. Also, a set of two genes was found to be better as control for normalization of the data. The expression of these genes (with more uniform expression) can be used for normalization of real-time PCR results for gene expression studies in a wide variety of samples in rice. 相似文献
84.
85.
Inner epidermal membrane of the onion bulb scales was studied as a natural polymer support for immobilization of the glucose oxidase (GOD) enzyme for biosensor application. Onion epidermal membrane was used for immobilization of glucose oxidase and was associated with dissolved oxygen (DO) probe for biosensor reading. Glucose was detected on the basis of depletion of oxygen, when immobilized GOD oxidizes glucose into gluconolactone. A wide detection range between 22.5 and 450 mg/dl was estimated from calibration plot. A single membrane was reused for 127 reactions with retention of approximately 90% of its initial enzyme activity. Membrane was stable for 45 days ( approximately 90% activity) when stored in buffer at 4 degrees C. Surface structure studies of the immobilized membranes were carried under SEM. To our knowledge, this is the first report on employing inner epidermal membrane of onion bulb scales as the solid support for immobilization of enzyme. 相似文献
86.
87.
Anjali Apte-Deshpnade Goutam Mandal Sudheerbabu Soorapaneni Bhaskarjyoti Prasad Jitendra Kumar Sriram Padmanabhan 《Biotechnology letters》2009,31(6):811-817
Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced
after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (~1 g/l) of extracellular glycosylated rSAK (~18 kDa) with
negligible plasminogen activation activity. Addition of tunicamycin during the induction phase resulted in expression of non-glycosylated
and highly active rSAK (~15 kDa) from the same clone. Two simple steps of ion-exchange chromatography produced an homogenous
rSAK of >95% purity which suitable for future structural and functional studies. 相似文献
88.
We studied the enhanced production of high quality biomass, δ-aminolevulinic acid (δ-ALA), bilipigments, and antioxidants
from five tropical blue green algae (cyanobacteria) in a full factorial design using free and immobilized cells in batch culture.
Production of nutraceuticals was high in spray dried powder prepared from immobilized cell cultures. Nostochopsis lobatus showed superiority over rest of the species with respect to bilipigments, δ-ALA, nutritive value, antioxidant capacity, and
ascorbate oxidase (APX) activity. Antioxidative capacity of phycobiliproteins extracted from these cyanobacteria (121.15 μM
TE/g, Nostoc verrucosum to 217.62 μM TE/g, Nostochopsis lobatus) was invariably higher than those observed for higher plant sources and substantially increased under immobilized cell culture
condition. Antioxidative enzyme, ascorbate oxidase remained stable in dry food preparations with considerably high activity
under immobilized cell preparations (APXmax, 3.40 μmol/min/mg chlorophyll). These observations have important connotations in light of upcoming food and nutraceutical
industries in the global market. Use of immobilized cells in batch culture could be an effective approach for scaling up production
for commercial use. 相似文献
89.
Foliar chemicals are variable within a plant and this may affect herbivore feeding preference. This study was carried out to quantify concentrations of primary (nitrogen, water, and total nonstructural carbohydrates) and secondary substances (sinigrin) in young and old leaves of Raphanus sativus L. and to evaluate performance and survival of a generalist herbivore Spodoptera litura F. feeding on them. Forty to 50-d-old R. sativus plants were used in both foliar chemical analysis and insect performance bioassays. Leaves located on the third to the sixth node from the base of the plant were defined as old leaves and the remaining leaves (from seventh node to the plant apex) of the plant were referred as young leaves in this study. All foliar chemicals except water differed significantly between young and old leaves. Moreover, young leaves were more nutritious but much more defended, based on sinigrin content, against S. litura than old leaves. Performance and survival of S. litura were reduced on young leaves as compared with old leaves. Male and female larval duration only differed significantly on young leaves. Female larval development time was longer than male development time on young leaves, but not on older leaves. Therefore, this study revealed that defenses in young leaves have differential effects upon male and female S. litura. 相似文献
90.
Jitendra Kumar Mishra Krishnananda Samanta Manish Jain Madhu Dikshit Gautam Panda 《Bioorganic & medicinal chemistry letters》2010,20(1):244-247
A diverse group of novel medium ring heterocycles derived from naturally abundant proteinogenic amino acids were evaluated for their potency towards antithrombotic activity. The more potent benzofused oxazepine and oxazocine scaffolds were diversified by incorporating different amino acids at the position number 3. Further the effect of ring size has also been taken into account and it was observed that the eight-membered oxazocines ane more potent compared to the corresponding oxazepines. 相似文献