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红细胞膜骨架与脂双层间存在着相互作用,其中带4.1蛋白与血型糖蛋白C/D间的相互作用对维持正常红细胞的形态和机械稳定性起着重要作用,研究表明,带4.1蛋白在血型糖蛋白C、D上的结合位点分别位于血型糖蛋白C的第82~98位氨基酸残基和血型糖蛋白D的第61~77位氨基酸残基. 相似文献
23.
盐或低温胁迫对花生幼苗下胚轴ATP酶和质膜中PIP2含量的影响 总被引:2,自引:0,他引:2
经6%-12%Dextran T70密度梯度离心,获得了纯度较高的7d龄花生幼苗下胚轴质膜和液泡膜制剂。150mmol/L NaCl或10℃低温处理花生幼苗24h,其下胚轴质膜上的Mg^2+激活的ATPase活性分别提高了37.6%和17.2%;Ca^2+-ATPase活性分别提高45.8%和33.6%。上述盐或低温处理也提高了液泡膜上Mg^2+激活的ATPase活性,分别为对照的141.2%和1 相似文献
24.
The simple design of traditional spinner flasks makes the on-line estimation of cellular metabolism impossible. An on-line estimation system has been developed and used for the monitoring of oxygen uptake rate (OUR) for insect cells growing in a modified spinner flask. Neglect of oxygen desorption from culture media is a common source of error in OUR measurements for Sf21 cells. Therefore, an algorithm was developed to compensate for the affect of such desorption process on the determination of OUR. A modified spinner flask was successfully used as a low-volume bioreactor for insect cell cultivation and the OUR measurement developed here is both convenient and reliable. 相似文献
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本文首次报道了五步蛇身体主要部位的数量性状与其排毒量的关系。其中我们发现与排毒量有关的部位是头积、体粗、体重、佛指长。 相似文献
27.
Conditions affecting direct gene transfer into rodent muscle in vivo. 总被引:23,自引:0,他引:23
This report extends our previous findings that mouse muscle cells in situ can take up naked DNA injected intramuscularly in vivo. Various conditions such as needle type, speed of injection, volume of injection fluid, tonicity of injection fluid, type of solute, type of muscle, physiologic condition of the muscle and age of the animals were appraised for their effect on the levels of luciferase activity expressed from the pRSVL plasmid. Specific conditions such as the use of normal saline as an injection fluid increased the efficiency of expression. The implantation of DNA pellets was an effective way to deliver DNA to muscle, especially for smaller muscle groups. Also, newborn and adult rat muscles expressed plasmid DNA delivered intramuscularly. 相似文献
28.
From ten genera and 146 bacterial strains, 22 strains producing alpha-amino acid ester hydrolase were selected. Among them, AS 1.586 and 41-2 were the best. The optimal conditions for synthesis of cephalexin by pseudomonas aeruginosa 1.204 were investigated. The optimal pH and temperature for enzymatic synthesis reaction was pH 6.8 and 25 degrees C, respectively. By using 1% 7-ADCA, 3% PGME and 4% biomass, about 70% of 7-ADCA was converted to cephalexin under the mentioned conditions. 相似文献
29.
Chromosomes of root tip cells ofAllium cepa andAllium sativum were studied in early, middle and late telophase to examine the organization of mitotic chromosomes, taking advantage of the naturally occurring chromosome dispersion during the process of decondensation in telophase. Longitudinal and transverse sections of telophase chromosomes viewed under the transmission electron microscope showed that mitotic chromosomes inAllium were composed of helically coiled 400–550 nm chromatin fibres. In some regions of the longitudinal sections, these chromatin fibres were seen to be orientated parallel to one another but formed roughly a right angle to the long axis of the chromosome. In transverse sections, the telophase chromosome appeared to have a hollow centre encircled by the 400–550 nm chromatin fibre which in turn was a hollow tube structure formed by the coiling of a thinner fibre of 170–200 nm. In addition, cross views of chromatin fibres of 170–200 nm and 50–70 nm were also identified in telophase chromosome preparations. These two organizational levels of chromatin fibres also showed a hollow centre. The process of decondensation of telophase chromosomes is described, and some morphological characteristics associated with the activities of chromosome decondensation are analysed. Based on the observations made onAllium chromosomes in this study, various models of chromosome organization are discussed. 相似文献
30.
道路绿化带可以净化空气,改善道路环境,道路中的小气候条件会改变道路污染物扩散方式和速度,进而会影响到绿化带对污染物净化效果。气象条件对道路绿地对大气污染物净化效果影响的研究将有助于了解道路绿地的净化途径,为改善城市道路环境提供依据。对太原市18个道路绿地气象因子和5种主要污染物浓度进行了观测。结果表明:夏季,太原市城市道路内各气象要素之间存在一定的相关性,气温和地温正相关显著,空气相对湿度与地温及气温呈显著和极显著负相关。大部分情况下,有绿地非机动车道污染物平均浓度低于无绿地非机动车道对照点平均浓度,即道路绿地起到了对道路污染物的净化作用。道路绿地对污染物的净化百分率与气象因子存在显著的回归关系,并可以建立达到统计显著水平的回归方程,但不同污染物受不同的主导气象因子影响。气象条件会影响道路绿地对道路污染物的净化效果,今后的城市建设和道路绿地规划中应更多地考虑气象条件对绿地净化效果的影响。 相似文献