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Young-Lai Cho Sung-Mo Hur Ji-Yoon Kim Ji-Hee Kim Dong-Keon Lee Jongeon Choe Moo-Ho Won Kwon-Soo Ha Dooil Jeoung Sanghwa Han Sungwoo Ryoo Hansoo Lee Jeong-Ki Min Young-Guen Kwon Dong-Hyun Kim Young-Myeong Kim 《The Journal of biological chemistry》2015,290(1):467-477
Ginsenoside Rg5 is a compound newly synthesized during the steaming process of ginseng; however, its biological activity has not been elucidated with regard to endothelial function. We found that Rg5 stimulated in vitro angiogenesis of human endothelial cells, consistent with increased neovascularization and blood perfusion in a mouse hind limb ischemia model. Rg5 also evoked vasorelaxation in aortic rings isolated from wild type and high cholesterol-fed ApoE−/− mice but not from endothelial nitric-oxide synthase (eNOS) knock-out mice. Angiogenic activity of Rg5 was highly associated with a specific increase in insulin-like growth factor-1 receptor (IGF-1R) phosphorylation and subsequent activation of multiple angiogenic signals, including ERK, FAK, Akt/eNOS/NO, and Gi-mediated phospholipase C/Ca2+/eNOS dimerization pathways. The vasodilative activity of Rg5 was mediated by the eNOS/NO/cGMP axis. IGF-1R knockdown suppressed Rg5-induced angiogenesis and vasorelaxation by inhibiting key angiogenic signaling and NO/cGMP pathways. In silico docking analysis showed that Rg5 bound with high affinity to IGF-1R at the same binding site of IGF. Rg5 blocked binding of IGF-1 to its receptor with an IC50 of ∼90 nmol/liter. However, Rg5 did not induce vascular inflammation and permeability. These data suggest that Rg5 plays a novel role as an IGF-1R agonist, promoting therapeutic angiogenesis and improving hypertension without adverse effects in the vasculature. 相似文献
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Hui Du Sunghan Kim Yoon-Sun Hur Myung-Sok Lee Suk-Ha Lee Choong-Ill Cheon 《Molecules and cells》2015,38(2):187-194
Thioredoxin (TRX) is a disulfide reductase present ubiquitously in all taxa and plays an important role as a regulator of cellular redox state. Recently, a redox-independent, chaperone function has also been reported for some thioredoxins. We previously identified nodulin-35, the subunit of soybean uricase, as an interacting target of a cytosolic soybean thioredoxin, GmTRX. Here we report the further characterization of the interaction, which turns out to be independent of the disulfide reductase function and results in the co-localization of GmTRX and nodulin-35 in peroxisomes, suggesting a possible function of GmTRX in peroxisomes. In addition, the chaperone function of GmTRX was demonstrated in in vitro molecular chaperone activity assays including the thermal denaturation assay and malate dehydrogenase aggregation assay. Our results demonstrate that the target of GmTRX is not only confined to the nodulin-35, but many other peroxisomal proteins, including catalase (AtCAT), transthyretin-like protein 1 (AtTTL1), and acyl-coenzyme A oxidase 4 (AtACX4), also interact with the GmTRX. Together with an increased uricase activity of nodulin-35 and reduced ROS accumulation observed in the presence of GmTRX in our results, especially under heat shock and oxidative stress conditions, it appears that GmTRX represents a novel thioredoxin that is co-localized to the peroxisomes, possibly providing functional integrity to peroxisomal proteins. 相似文献
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Kim SN Shim HP Jeon BN Choi WI Hur MW Girton JR Kim SH Jeon SH 《Molecules and cells》2011,32(6):549-554
Polycomb group (PcG) proteins maintain the spatial expression patterns of genes that are involved in cell-fate specification
along the anterior-posterior (A/P) axis. This repression requires cis-acting silencers, which are called PcG response elements (PREs). One of the PcG proteins, Pleiohomeotic (Pho), which has
a zinc finger DNA binding protein, plays a critical role in recruiting other PcG proteins to bind to PREs. In this study,
we characterized the effects of a pho mutation on embryonic segmentation. pho maternal mutant embryos showed various segmental defects including pair-rule gene mutant patterns. Our results indicated
that engrailed and even-skipped genes were misexpressed in pho mutant embryos, which caused embryonic segment defects. 相似文献
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The complete mitochondrial genome (JN415127) was sequenced and annotated newly from an individual of Hynobius yangi populations which were collected in Ulju-gun, Korea. The total length of the H. yangi genome is 16,403 bp, with a total base composition of 33.5% A, 32.3% T, 20.5% C, and 13.6% G. 相似文献
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