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91.
Hao Bing Li Guo Qiang Xie Jun Ma Gui Ru Liu Shu Min Wen Tomohiro Ban Sukumar Chakraborty Chun Ji Liu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):941-950
Fusarium head blight (FHB) and crown rot (CR) are two wheat diseases caused by the same Fusarium pathogens. Progress towards CR resistance could benefit from FHB-resistant germplasm if the same genes are involved in resistance
to these two different diseases. Two independent studies were conducted to investigate the relationship between host resistances
to these two diseases. In the first study 32 genotypes were assessed and no significant correlation between their reactions
to FHB and CR was detected. The second study was based on a QTL analysis of a doubled haploid population derived from a variety
with resistance to both diseases. Results from this study showed that loci conferring resistance to FHB and CR are located
on different chromosomes. Together, these results suggest that, despite a common aetiology, different host genes are involved
in the resistance against FHB and CR in wheat. Thus, although it is possible that genes affecting both diseases may exist
in other germplasm or under different conditions, separate screening seems to be needed in identifying sources of CR resistance. 相似文献
92.
Xianghong Li John Gui Hong Yang Wenjun Wu Fuyou Li He Tian Chunhui Huang 《Inorganica chimica acta》2008,361(9-10):2835-2840
Three new amphiphilic ruthenium complexes (Ru-1, Ru-2 and Ru-3) based on phenanthrenyl derivatives have been synthesized and used as photosensitizers for dye-sensitized solar cells (DSSCs). The ruthenium complex Ru-1 containing a carbazole group showed especially improved photophysical properties (red-shifted metal-to-ligand charge-transfer transition absorptions and enhanced molar extinction coefficients) and interesting electrochemical properties, resulting in its improved open circuit potential and high overall light-to-electric power conversion efficiency of 5.3% (AM1.5, 75 mW/cm2). These facts indicate that the carbazole-based phenanthrenyl ruthenium complex is a promising candidate for improving the conversion efficiency of dye-sensitized solar cells. 相似文献
93.
Extraction of intracellular protein from Escherichia coli is traditionally achieved by mechanical, chemical or enzymatic disruption technology. In this study, a novel thermolysis method was used to disrupt E. coli cells to release a recombinant thermostable esterase. We found that heat treatment of E. coli was highly effective to destroy the integrity of bacterial cell walls and release the recombinant hyperthermophilic esterase at temperatures above 60 degrees C. The effects of temperature, pH and cell concentration on the efficiency of cell disruption were examined. The most effective temperature for cell disruption was at 80 degrees C. The pH and cell concentration had only minor effect on the release of the hyperthermophilic esterase. In addition, we found that the hyperthermophilic esterase could be purified at the early stage of the thermolysis, which is a major advantage of the thermolysis method. Finally, a comparison between thermolysis and traditional methods for the disruption of cells and the release of the thermostable enzyme was made. 相似文献
94.
Yin J Xia JH Du XZ Liu J Zhou L Hong YH Gui JF 《The International journal of developmental biology》2007,51(8):761-769
Midkine (Mdk) genes have been revealed to have different expression patterns in vertebrates and therefore, additional studies on Mdk expression patterns are required in more species. In this study, CagMdkb has been cloned and characterized from a SMART cDNA library of 10-somite stage embryos of Carassius auratus gibelio. Its full length cDNA is 1091 bp and encodes a sequence of 147 amino acids, which shows 97.3% identity to zebrafish Mdkb on the amino acid level. RT-PCR analysis reveals that CagMdkb is first transcribed in gastrula embryos and maintains a relatively stable expression level during subsequent embryogenesis. Western blot analysis reveals a 19 kDa maternal CagMdkb protein band and the zygotic CagMdkb protein is expressed from gastrula stage. At around 10 somite stage, the 19 kDa CagMdkb is processed to another protein band of about 17 kDa, which might be the secreted form with the 21-residue signal peptide removed. With immunofluorescence analysis, maternal CagMdkb protein was found to be localized in each blastamere cell of early embryos. The zygotic CagMdkb positive fluorescence signal was detected from a pair of large neurons at 18-somite stage. At the later stages, CagMdkb protein was also extended to numerous small neurons in the forebrain, midbrain and hindbrain, as well as to nerve fibers in the spinal cord. Co-localization with 3A10 antibody revealed CagMdkb immunoreactivity on developing Mauthner neurons, a member of reticulospinal neurons. In addition, ectopic expression of CagMdkb in early embryos of gibel carp and zebrafish suppressed head formation and CagMdkb function was found to depend on secretory activity. All these findings indicate that CagMdkb plays an important role in neural development during gibel carp embryogenesis and there is functional conservation of Mdkb in fish head formation. 相似文献
95.
Wang HY Zhou L Gui JF 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2007,146(1):47-52
Previous studies have demonstrated that germinal vesicle of amphibian oocyte contains small nuclear ribonucleoprotein polypeptide C (SNRPC). In this study, a putative member of SNRPC was identified from Carassius auratus gibelio oocyte cDNA library. Its full-length cDNA has an open reading frame of 201 nt for encoding a peptide of 66 aa, a short 5'-UTR of 19 nt and a long 3'-UTR of 347 nt including a polyadenylation signal and poly- (A) tail, and the deduced amino acid sequence has 47% identity with the C-terminal of the zebrafish small nuclear ribonucleoprotein polypeptide C. Western blot analysis revealed its oocyte-specific expression. Immunofluorescence localization indicated that its gene product localized to numerous nucleoli within the oocytes and showed dynamic changes with the nucleoli during oocyte maturation. RT-PCR and Western blot analysis further revealed its constant presence in the oocytes and in the embryos until hatching. The data suggested that the newly identified CagOSNRPC might be a nucleolar protein. 相似文献
96.
97.
Deng Nian-Hua Luo Wen Gui Dan-Dan Yan Bin-Jie Zhou Kun Tian Kai-Jiang Ren Zhong Xiong Wen-Hao Jiang Zhi-Sheng 《Molecular and cellular biochemistry》2022,477(1):255-265
Molecular and Cellular Biochemistry - Diabetic cardiomyopathy (DCM) is a cardiovascular complication that tends to occur in patients with diabetes, obesity, or insulin resistance, with a higher... 相似文献
98.
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100.
Li‐Fei Bai Hua Zhao Cheng‐Yi Tang Yan‐Jun Pang Rong‐Wu Yang Xiao‐Ming Wang Gui‐Hua Lu Yong‐Hua Yang 《Chirality》2015,27(3):274-280
In this study, a shikonin ester derivative, compound 3g , was selected to evaluate its anticancer activities and we found that compound 3g exhibited better antitubulin activities against the human HepG2 cell line with an IC50 value of 1.097 μM. Furthermore, the inhibition of tubulin polymerization results indicated that compound 3g demonstrated the most potent antitubulin activity (IC50 = 13.88), which was compared with shikonin and colchicine as positive controls (IC50 = 25.28 μM and 22.56 μM), respectively. Compound 3g was simulated to have good binding site with tubulin and arrested the cell cycle at G2/M phase, which also induces apoptosis in HepG2 cells, in which P53 and members of Bcl‐2 protein family were both involved in the progress of apoptosis revealed by western blot. Confocal microscopy observations revealed compound 3g targeted tubulin and altered its polymerization by interfering with microtubule organization. Based on these results, compound 3g functions as a potent anticancer agent targeting tubulin. Chirality 27:274–280, 2015.. © 2015 Wiley Periodicals, Inc. 相似文献