生物功能化色谱技术研究胰岛素与其受体间的相互作用

生物功能化色谱技术是通过模拟细胞膜表面环境,在色谱系统内实现研究生物分子间相互作用过程的新技术。利用生物功能化色谱方法研究了胰岛素与其受体间的相互作用。将提取出的胰岛素受体混合物固定化在人工膜(Immobilized artificial membrane, IAM)的表面,并确定了固定化的最佳pH值为7.2,最佳离子强度为20 mmol,最佳有机溶剂浓度为2% (v/v)。通过区带洗脱法确定了胰岛素受体的存在及其与胰岛素间存在相互作用;通过前沿分析法确定了胰岛素受体与胰岛素间相互作用的结合常数大小约为0.701 nmol/L。     

芦笋提取液体外抗氧化、细胞毒作用及抑制α-葡萄糖苷酶活性研究

本文研究了芦笋提取液的抗氧化活性、对结肠癌细胞的细胞毒作用及对α-葡萄糖苷酶活性的抑制作用。通过研究其对DPPH自由基、羟基自由基及亚硝酸盐的清除能力来评价芦笋提取液的抗氧化活性,通过MTT法研究了芦笋提取液对结肠癌细胞株的细胞毒作用,通过α-葡萄糖苷酶体外抑制试验观察了芦笋提取液对大鼠小肠α-葡萄糖苷酶活性的影响。研究结果表明,芦笋提取液清除羟基自由基和亚硝酸盐的能力强于清除DPPH自由基的能力,一定浓度的芦笋提取液对结肠癌细胞株具有较强的细胞毒作用,并能够有效抑制大鼠小肠α-葡萄糖苷酶活性。     

国人睾丸、附睾和输精管的水解酶的组织化学观察

本文报导国人睾丸、附睾和输精管的ＮＳＥ,ＡＣｈＥ,ＣｈＥ,ＡＬＰ,ＡＣＰ,５＇－Ｎａｓｅ,Ｇ－６－Ｐａｓｅ,β－ＧＡ,β－ＧＲ,ＡＰ－Ｍ,ＡＴＰａｓｅ和ＴＰＰａｓｅ水解酶的组织化学活性、结果显示：睾丸曲细精管的ＡＬＰ,５＇－Ｎａｓｅ和ＡＴＰａｓｅ;睾丸间质细胞的ＮＳＥ,ＡＬＰ,ＡＴＰａｓｅ和ＡＣＰ;睾丸间质中的ＮＳＥ,ＡＬＰ和ＡＴ－Ｐａｓｃ;睾丸输出小管和附睾管上皮的ＮＳＥ,ＡＬＰ,ＡＣＰ,５’－Ｎａｓｅ,β－ＧＡ,β－ＧＲ,ＡＴＰａｓｅ和Ｔｐｐａｓｅ;附睾头部间质中的ＮＳＥ,ＡＣｈＥ,ＡＬＰ,和ＡＴＰａｓｅ;输精管上皮细胞的ＡＴＰａｓｅ的酶活性均呈强阳性或极强阳性。说明人类睾丸、附睾和输精管含有丰富的水解酶,尤其是附睾头部的输出小管、附睾管和头质均含有种类多活性高的水解酶,在精子的功能成熟上起了重要的作用,提示其可能作为生育与不育诊治中的重要指标。     

Biocatalytic Production of Acyclic Bis[bibenzyls] from Dihydroresveratrol by Crude Momordica charantia Peroxidase

Biotransformation of dihydroresveratrol by crude Momordica charantia peroxidase provided six new acyclic bis[bibenzyls] 1 – 6 . Their structures were established on the basis of NMR and MS analyses as C? C, C? O? C, and C? CH₂? C dimers of dihydroresveratrol. Compounds 1 – 6 were tested for antiproliferative activity against human prostate cancer PC3 cell line in vitro, and 2 and 6 were found to be more potent than the parent compound.     

Acute administration of fluoxetine normalizes rapid eye movement sleep abnormality, but not depressive behaviors in olfactory bulbectomized rats

In humans, depression is associated with altered rapid eye movement (REM) sleep. However, the exact nature of the relationship between depressive behaviors and sleep abnormalities is debated. In this study, bilateral olfactory bulbectomy (OBX) was carried out to create a model of depression in rats. The sleep-wake profiles were assayed using a cutting-edge sleep bioassay system, and depressive behaviors were evaluated by open field and forced swimming tests. The monoamine content and monoamine metabolite levels in the brain were determined by a HPLC-electrochemical detection system. OBX rats exhibited a significant increase in REM sleep, especially between 15:00 and 18:00 hours during the light period. Acute treatment with fluoxetine (10 mg/kg, i.p.) immediately abolished the OBX-induced increase in REM sleep, but hyperactivity in the open field test and the time spent immobile in the forced swimming test remained unchanged. Neurochemistry studies revealed that acute administration of fluoxetine increased serotonin (5-HT) levels in the hippocampus, thalamus, and midbrain and decreased levels of the 5-HT metabolite 5-hydroxyindoleacetic acid (5-HIAA). The ratio of 5-HIAA to 5-HT decreased in almost all regions of the brain. These results indicate that acute administration of fluoxetine can reduce the increase in REM sleep but does not change the depressive behaviors in OBX rats, suggesting that there was no causality between REM sleep abnormalities and depressive behaviors in OBX rats.