Internalization of ferromagnetic nanowires by different living cells

The ability of living cells, either adherent or suspended, to internalize nickel nanowires is demonstrated for MC3T3-E1, UMR106-tumour and Marrow-Stromal cells. Nanowires were produced by electrodeposition, 20 μm long and 200 nm in diameter. Cell separation and manipulation was achieved for the three cell types. Applied magnetic field successfully oriented the internalized nanowires but no clear anisotropy is induced on the adherent cells. Nanowires tend to bind to cytoplasm metalloproteins and trigger lysosome reorganization around the nucleus. This work demonstrates the applications of nanowires in adherent and suspended cells for cell separation and manipulation, and further explore into their role in nanobiotechnology.     

Bayesian interval mapping of count trait loci based on zero-inflated generalized Poisson regression model

Count phenotypes with excessive zeros are often observed in the biological world. Researchers have studied many statistical methods for mapping the quantitative trait loci (QTLs) of zero-inflated count phenotypes. However, most of the existing methods consist of finding the approximate positions of the QTLs on the chromosome by genome-wide scanning. Additionally, most of the existing methods use the EM algorithm for parameter estimation. In this paper, we propose a Bayesian interval mapping scheme of QTLs for zero-inflated count data. The method takes advantage of a zero-inflated generalized Poisson (ZIGP) regression model to study the influence of QTLs on the zero-inflated count phenotype. The MCMC algorithm is used to estimate the effects and position parameters of QTLs. We use the Haldane map function to realize the conversion between recombination rate and map distance. Monte Carlo simulations are conducted to test the applicability and advantage of the proposed method. The effects of QTLs on the formation of mouse cholesterol gallstones were demonstrated by analyzing an mouse data set.     

A novel approach to prepare extended DNA fibers in plants.

BACKGROUND: The extended DNA fiber preparation procedure is still imperfect in plants due to the existence of a hard cell wall; thus, high quality of extended DNA fibers for fluorescence in situ hybridization (FISH) analysis is often difficult to be obtained rapidly and efficiently. In this study we have developed a fast and widely effective method to prepare DNA fibers from various plant species and the fibers are suitable for fiber FISH mapping. METHODS: Fresh young leaves were chopped with a sharp sterile scalpel in a Petri dish that contained ice-cold nucleus isolation buffer followed by filtration through 33-mum nylon mesh. Nuclei were obtained by centrifuging the filtrates at high speed (16,000g) for 40 s. Nucleus lysis buffer (0.5% sodium dodecylsulfate, 5 mM ethylenediaminetetraacetic acid, 100 mM Tris, pH7.0) was added to nuclei on slides, and DNA fibers were dragged and extended with a clean coverslip. RESULTS: The key of this method is that liquid nitrogen grinding of leaves is replaced by chopping with a blade in ice-cold nucleus isolation buffer. With the liquid nitrogen method, over- or under-grinding of leaves occurs more frequently, and DNA fibers with the desired quality are not obtained easily. In contrast, it is easier to release nuclei from cells in nucleus isolation buffer by chopping, which results in fewer nuclei being destroyed. Highly extended, intact, and long DNA fibers can be generated to a great probability with this method. In addition, this method is very simple and rapid, requiring only 20 min for the entire process, and is also safe because poisonous mercaptoethanol is replaced by dithiothreitol. The results of fiber-FISH with maize genomic DNA and 45S rDNA as probes showed that DNA fiber size as long as 1.96 Mb could be measured. The successful and reliable preparation of maize, wild rice, and barley DNA fibers suitable for FISH mapping proves that this technique is a widely effective approach for obtaining extended DNA fibers in plants. CONCLUSIONS: A simple, rapid, safe, and widely effective method for getting extended DNA fibers has been developed in plants. (c) 2005 Wiley-Liss, Inc.     

Host sunflower-induced silencing of parasitism-related genes confers resistance to invading Orobanche cumana

Orobanche cumana is a holoparasitic plant that attaches to host–plant roots and seriously reduces the yield of sunflower (Helianthus annuus L.). Effective control methods are lacking with only a few known sources of genetic resistance. In this study, a seed-soak agroinoculation (SSA) method was established, and recombinant tobacco rattle virus vectors were constructed to express RNA interference (RNAi) inducers to cause virus-induced gene silencing (VIGS) in sunflower. A host target gene HaTubulin was systemically silenced in both leaf and root tissues by the SSA–VIGS approach. Trans-species silencing of O. cumana genes were confirmed for 10 out of 11 target genes with silencing efficiency of 23.43%–92.67%. Knockdown of target OcQR1, OcCKX5, and OcWRI1 genes reduced the haustoria number, and silencing of OcEXPA6 caused further phenotypic abnormalities such as shorter tubercles and necrosis. Overexpression of OcEXPA6 caused retarded root growth in alfalfa (Medicago sativa). The results demonstrate that these genes play an important role in the processes of O. cumana parasitism. High-throughput small RNA (sRNA) sequencing and bioinformatics analyses unveiled the distinct features of target gene-derived siRNAs in O. cumana such as siRNA transitivity, strand polarity, hotspot region, and 21/22-nt siRNA predominance, the latter of which was confirmed by Northern blot experiments. The possible RNAi mechanism is also discussed by analyzing RNAi machinery genes in O. cumana. Taken together, we established an efficient host-induced gene silencing technology for both functional genetics studies and potential control of O. cumana. The ease and effectiveness of this strategy could potentially be useful for other species provided they are amenable to SSA.

Knockdown of several parasitism-related genes endows sunflower with resistance to invading broomrape.     

Double-flow convolutional neural network for rapid large field of view Fourier ptychographic reconstruction

Fourier ptychographic microscopy is a promising imaging technique which can circumvent the space-bandwidth product of the system and achieve a reconstruction result with wide field-of-view (FOV), high-resolution and quantitative phase information. However, traditional iterative-based methods typically require multiple times to get convergence, and due to the wave vector deviation in different areas, the millimeter-level full-FOV cannot be well reconstructed once and typically required to be separated into several portions with sufficient overlaps and reconstructed separately, which makes traditional methods suffer from long reconstruction time for a large-FOV (of the order of minutes) and limits the application in real-time large-FOV monitoring of live sample in vitro. Here we propose a novel deep-learning based method called DFNN which can be used in place of traditional iterative-based methods to increase the quality of single large-FOV reconstruction and reducing the processing time from 167.5 to 0.1125 second. In addition, we demonstrate that by training based on the simulation dataset with high-entropy property (Opt. Express 28, 24 152 [2020]), DFNN could has fine generalizability and little dependence on the morphological features of samples. The superior robustness of DFNN against noise is also demonstrated in both simulation and experiment. Furthermore, our model shows more robustness against the wave vector deviation. Therefore, we could achieve better results at the edge areas of a single large-FOV reconstruction. Our method demonstrates a promising way to perform real-time single large-FOV reconstructions and provides further possibilities for real-time large-FOV monitoring of live samples with sub-cellular resolution.     

半红树植物玉蕊对淹水-盐度胁迫的生长及生理响应

玉蕊(Barringtonia racemosa)是典型的半红树植物,具有重要的药用及观赏价值,目前已被列为濒危植物,亟需保护.为探明不同淹水水位、淹水时长以及水体盐度对玉蕊生长的影响,筛选出适合玉蕊生存的最佳环境组合,该文以二年生玉蕊实生苗为试验材料,模拟全日潮,采用L9(34)正交试验设计,对其生长及生理指标进行分...     

High level serum neutralizing antibody against HIV-1 in Chinese long-term non-progressors

NAb have been considered to be an important component of a protective immune response to HIV-1, yet the relationship between the capacity of HIV-1 NAb, the conserved neutralization epitopes and disease progression has been unclear. To gain a better understanding of the protective roles that NAb and conserved neutralization epitopes could play in LTNP, twenty-eight HIV-1-infected subjects were investigated by evaluation of the concentrations of HIV-1 NAb and conserved neutralization epitopes, using single-round PBMC neutralization assay and sequencing. Our study revealed that the concentration of NAb in LTNP was significantly higher than that in subjects with asymptomatic HIV (P < 0.05) and AIDS (P < 0.01). No amino acids substitutions were found in the conserved epitopes of the HIV-1 gp120 region in LTNP, whereas the viruses circulating both in persons with asymptomatic HIV and those with AIDS had amino acid substitutions in their conserved neutralization epitopes. This study suggests that high levels of NAb and stable epitopes in gp120 could play a crucial role in protection against disease progression.     

膀胱功能代偿系统的研究

本文对截瘫病人膀胱功能障碍的重建机理和方法进行了综合分析,对临床上解决膀胱功能代偿的各种途径加以比较,提出膀胱功能代偿系统-膀胱控制器的新设计,它将会使我国广大的膀胱功能障碍患者受益。     

Analysis of genetic factors that control shoot mineral concentrations in rapeseed (Brassica napus) in different boron environments

Mineral nutrients are essential for plant cell function, and understanding the genetic and physiological basis of mineral concentration is therefore important for the development of nutrient-efficient crop varieties that can cope with a shortage of mineral resources. In the present study, we investigated the profiles of B, Ca, Fe, Cu, Mg, P and Zn concentrations in shoots and analyzed the genetic variation in a rapeseed (Brassica napus) double haploid population at normal and deficient boron (B) levels in hydroponic conditions. Significant correlations between the concentrations of different minerals, such as Ca and Mg, Ca and P, and Cu and Fe, existed in both B environments. A total of 35 quantitative trait loci (QTL) and 74 epistatic interaction pairs for mineral concentrations were identified by whole genome analysis of QTL and epistatic interactions. The individual phenotypic contributions of the QTL ranged from 4.4% to 19.0%, and the total percentage of genetic variance that was due to QTL and epistatic interactions varied from 10.4% to 82.4%. Most of these QTL corresponded specifically to one of the two B conditions except for one stable main-effect P-QTL across the B environments. Three QTL for Ca and Mg were found to co-localize under normal B condition. These results revealed that genetic factors control mineral homeostasis in plants and multigenes involving ion transport are required to regulate mineral balance in plants under conditions of diverse nutrient stress. In addition, 26 genes involved in ion uptake and transport in Arabidopsis thaliana were in silico mapped onto the QTL intervals of B. napus by comparative genomic analysis. These candidate orthologous genes in B. napus allowed the selection of genes involved in the controlling mineral concentration that may account for the identified QTL.