江西黄毛楤木氨基酸及微量元素的测定分析

用氨基酸分析仪、原子吸收分光光度计,对江西黄毛楤木中的营养成份进行分析研究。分别测定黄毛楤木根皮中氨基酸及微量元素含量。结果显示黄毛楤木根皮中含有16种氨基酸,总量为4.57mg·g-1,人体必需的8种氨基酸齐全,质量分数为1.71mg·g-1,占氨基酸总量的37.33％;含有多种人体必需的微量元素和宏量元素,尤其是zn,cu,K,Mg含量较其它种楤木要高。江西黄毛楤木富含氨基酸、微量元素,营养成分丰富,作为一种营养、保健、医药资源大有开发利用价值。     

魔芋无土栽培营养液配方研究初报

分别采用全MS、1/2MS、1/5MS、1/10MS(含大量元素、微量元素和铁盐)、1/10MS大量元素营养液(不含微量元素和铁盐)定期浇灌用蛭石栽培的不同级别的魔芋,前50d生长状况没有差异,50-80d以1/2MS-1/10MS营养液为佳,80-110d时1-5MS-1/10MS生长较好;魔芋在缺乏微量元素的营养液中后期生长不良,提前倒苗;产量分析结果表明:35g以上的种芋以1/2MS最高,35g以下的种芋以1/10MS最高,魔芋的耐盐浓度在2.25‰以下,高盐浓度对块茎的形成有毒害作用,造成产量大幅度下降,添加微量元素和铁有较好的增产作用。     

Enjeux de la biologie délocalisée en milieu hospitalier et contractualisation

La réussite d’une délocalisation des analyses de biologie médicale est la résultante d’une volonté de coopération entre les trois partenaires que sont le clinicien, le biologiste et les services administratifs. Elle s’appuie sur la répartition clairement définie des responsabilités en termes d’utilisation de résultats et de management, la mise en place de procédures de fonctionnement et un suivi continu de la totalité du processus. Elle ne peut se passer d’une liaison informatique en temps réel avec le laboratoire qui permet de respecter les règles de l’assurance qualité et du GBEA. Bien ma?trisée, et appliquée à bon escient, elle peut représenter une solution pour améliorer la prise en charge du patient.     

Transacetylation of carbohydrates in organic solvent catalysed by O-acetylpeptidoglycan esterase from Neisseria gonorrhoeae

The potential of the Neisseria gonorrhoeae O-acetylpeptidoglycan esterase (Ape1a) for catalysing transacetylations in organic solvents with a number of carbohydrate acceptors was investigated. The performance of the enzyme was observed to improve as the polarity index of the solvent increased. The best transacetylation conditions were determined to be a 1:6 phosphate buffer/ethyl acetate system, where Ape1a catalysed approximately 28% acetylation of 4-methylumbelliferyl-N-acetylglucosamine using p-nitrophenyl acetate as donor. Further analysis of the acetylated products by reverse phase HPLC and ESI-mass spectrometry confirmed the presence of monoacetylated 4-methylumbelliferyl-N-acetylglucosamine. Under identical reaction conditions, the enzyme also performed transacetylations using ethyl acetate or vinyl acetate as donor. These results demonstrated the feasibility of using the bacterial cell wall enzyme Ape1a to generate hitherto unattainable compounds which may be used as antagonists of peptidoglycan-metabolizing enzymes.     

Linked Production of Pyroglutamate-Modified Proteins via Self-Cleavage of Fusion Tags with TEV Protease and Autonomous N-Terminal Cyclization with Glutaminyl Cyclase In Vivo

Overproduction of N-terminal pyroglutamate (pGlu)-modified proteins utilizing Escherichia coli or eukaryotic cells is a challenging work owing to the fact that the recombinant proteins need to be recovered by proteolytic removal of fusion tags to expose the N-terminal glutaminyl or glutamyl residue, which is then converted into pGlu catalyzed by the enzyme glutaminyl cyclase. Herein we describe a new method for production of N-terminal pGlu-containing proteins in vivo via intracellular self-cleavage of fusion tags by tobacco etch virus (TEV) protease and then immediate N-terminal cyclization of passenger target proteins by a bacterial glutaminyl cyclase. To combine with the sticky-end PCR cloning strategy, this design allows the gene of target proteins to be efficiently inserted into the expression vector using two unique cloning sites (i.e., SnaB I and Xho I), and the soluble and N-terminal pGlu-containing proteins are then produced in vivo. Our method has been successfully applied to the production of pGlu-modified enhanced green fluorescence protein and monocyte chemoattractant proteins. This design will facilitate the production of protein drugs and drug target proteins that possess an N-terminal pGlu residue required for their physiological activities.     

Ontogeny of light response in the early life history of the red king crab Paralithodes camtschaticus (Anomura: Lithodidae)

Phototactic responses of light-adapted zoeae IV, glaucothoe, and first stage juveniles of the red king crab to three intensities of white light were quantitatively measured under laboratory conditions. All stages observed were photopositive to all light intensities tested, except for late glaucothoe (10 days since moulting) which did not respond to light stimuli. Phototactic response changed in the early life history of the red king crab. The extent of photopositive movement decreased after each metamorphosis. Peak phototactic response in zoea IV were observed at a light intensity of 1.9 × 10¹³ q cm^-2 s^-1, in early glaucothoe at 1.1 × 10¹⁰ q cm^-2 s^-1 and in juveniles at 1.3 × 10⁹ q cm^-2 s^-1. The data on behavioural responses to light may provide a better understanding of the early life history, survival and recruitment of the red king crab and assist the development of feasible methods and techniques for aquaculture of this species.     

The cytotaxonomic study of theVicia cracca complex 2. The taxa of the southern part of northern Europe

In the southern parts of Scandinavia there occur two species of theVicia cracca L. complex:Vicia cracca L., represented by the chromosome race 2n=28, andV. oreophila ?ertová. WhereasV. oreophila predominantly inhabits habitats of primary character, especially near the coast, the tetraploid race ofV. cracca, which is the commonest in other parts of area, occurs predominantly on sites of secondary character, such as the borders of paths, roads, meadows and bushy places. The two species differ in several characters.     

青海南部三种紫菀属植物的核型研究

报道了青海南部三种紫苑属植物的核型,染色体间期和前期 色体分别为复杂型和中间型,染色体数目均为２ｎ＝１８,基数为ｘ＝９,中期染色体主要由中部与亚中着丝点染色体组成。