全文获取类型
收费全文 | 525篇 |
免费 | 72篇 |
国内免费 | 12篇 |
出版年
2022年 | 3篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 8篇 |
2017年 | 3篇 |
2016年 | 14篇 |
2015年 | 8篇 |
2014年 | 13篇 |
2013年 | 21篇 |
2012年 | 18篇 |
2011年 | 26篇 |
2010年 | 13篇 |
2009年 | 13篇 |
2008年 | 18篇 |
2007年 | 21篇 |
2006年 | 109篇 |
2005年 | 25篇 |
2004年 | 35篇 |
2003年 | 44篇 |
2002年 | 10篇 |
2001年 | 19篇 |
2000年 | 13篇 |
1999年 | 26篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 7篇 |
1994年 | 7篇 |
1993年 | 4篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1985年 | 3篇 |
1983年 | 4篇 |
1981年 | 3篇 |
1980年 | 10篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 18篇 |
1976年 | 11篇 |
1975年 | 10篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 4篇 |
1965年 | 7篇 |
排序方式: 共有609条查询结果,搜索用时 859 毫秒
21.
D. Ćalić S. Zdravković-Korać S. Jevremović M. Guć-Šćekić Lj. Radojević 《Biologia Plantarum》2003,47(2):289-292
Suspension culture was more efficient method for haploid production than anther culture. All analysed androgenic regenerants
originating from embryogenic microspores in suspension culture of Aesculus hippocastanum L. had a haploid number of chromosomes (n=20), while 50 % of those derived from anther culture were diploids.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
22.
Vávrová J Mareková-Rezácová M Vokurková D Szkanderová S Psutka J 《Radiation and environmental biophysics》2003,42(3):193-199
Most cell lines that lack functional p53 protein are arrested in the G2 phase of the cell cycle due to DNA damage. It was previously found that the human promyelocyte leukemia cells HL-60 (TP53 negative) that had been exposed to ionizing radiation at doses up to 10 Gy were arrested in the G2 phase for a period of 24 h. The radioresistance of HL-60 cells that were exposed to low dose-rate gamma irradiation of 3.9 mGy/min, which resulted in a pronounced accumulation of the cells in the G2 phase during the exposure period, increased compared with the radioresistance of cells that were exposed to a high dose-rate gamma irradiation of 0.6 Gy/min. The D0 value (i.e. the radiation dose leading to 37% cell survival) for low dose-rate radiation was 3.7 Gy and for high dose-rate radiation 2.2 Gy. In this study, prevention of G2 phase arrest by caffeine (2 mM) and irradiation of cells with low dose-rate irradiation in all phases of the cell cycle proved to cause radiosensitization (D0=2.2 Gy). The irradiation in the presence of caffeine resulted in a second wave of apoptosis on days 5–7post-irradiation. Caffeine-induced apoptosis occurring later than day 7 post-irradiation is postulated to be a result of unscheduled DNA replication and cell cycle progress. 相似文献
23.
槭属(Acer L.)属槭树科(Aceraceae),200种,分布于亚、欧、北美和非洲北缘。本文研究了槭属的系统演化、地理分布、起源与扩散。认为:(1)槭树科与无患于科关系密切,槭属是槭树科2属中较进化的类群。(2)在原始而典型的槭属植物的基础上,槭属沿花的各部减少,有的器官甚至向完全退化的方向演化,但也有少数向增加数目的方向特化。(3)讨论了槭属4亚属23组的演化趋势,并绘制出其系统演化图。(4)槭属起源于侏罗纪的中国四川东部、湖北、湖南及其邻近地区,并向西、东北和南方扩散而进入西亚、欧洲、非洲北缘、北美洲和马来半岛至印尼。 相似文献
24.
Ori Rogowski - Ori Rogowski Itzhak Shapira should both be considered first authors Itzhak Shapira Sharon Toker Samuel Melamed Arie Shirom David Zeltser Shlomo Berliner 《Biomarkers》2007,12(6):645-656
The objective of our study was to determine whether the very low concentrations of C-reactive protein (CRP) detected by high-sensitivity CRP (hs-CRP) assays that one encounters from time to time in apparently healthy individual represent a physiological status or are just a reflection of an improved general health profile. The concentration of hs-CRP was determined by using the Behring BN II nephelometer. The arbitrary cut-off point of hs-CRP (≤0.16mgl-1) was determined at the lower detection level of the assay. A total of 6588 apparently healthy individuals were screened following exclusion of recent infection/inflammation by using a detailed questionnaire. One hundred and sixty (2.4%) individuals out of the above-mentioned cohort presented hs-CRP concentrations of ≤0.16mgl-1. They were found to be significantly younger and lean, had an improved lipid profile and an attenuated acute-phase response in terms of lower erythrocyte sedimentation rate and fibrinogen concentration as well as white blood cell count. In addition, these individuals had less atherothrombotic risk factors, except for smoking habits which were as frequent as in those of individuals with a higher hs-CRP concentration. After calculating the concentration of this biomarker following multiple adjustments, the individuals with very low CRP remained with a very low value despite the multiplicity of the adjustments. We raise the possibility that this particular low concentration might represent a physiological status and is not necessarily a result of the improved general health profile per se. 相似文献
25.
Dipeptidyl peptidases III (DPPs III) form a distinct metallopeptidase family characterized by the unique HEXXGH motif. High susceptibility to inactivation by organomercurials suggests the presence of a reactive cysteine residue(s) in, or close to, their active site. Yeast DPP III contains five Cys, none of which is absolutely conserved within the family. In order to identify reactive residue(s), site-directed mutagenesis on yeast His6-tagged DPP III was employed to substitute specifically all five cysteine residues to serine. The variant enzymes thus obtained were enzymatically active and showed an overall structure not greatly affected by the mutations as judged by circular dichroism. Analysis by native and SDS-PAGE under non-reducing conditions revealed the existence of a monomeric and dimeric form in all DPP III proteins except in the C130S, implying that dimerization of yeast DPP III is mediated by the surface-exposed cysteine 130. 相似文献
26.
27.
İbrahim Horo Erdal Bedir Angela Perrone Fevzi Özgökçe Sonia Piacente Özgen Alankuş-Çalışkan 《Phytochemistry》2010,71(8-9):956-963
Six cycloartane-type triterpene glycosides were isolated from Astragalus icmadophilus along with two known cycloartane-type glycosides, five known oleanane-type triterpene glycosides and one known flavonol glycoside. The structures of the six compounds were established as 3-O-[α-L-arabinopyranosyl-(1 → 2)-O-3-acetoxy-α-L-arabinopyranosyl]-6-O-β-D-glucopyranosyl-3β,6α,16β,24(S),25-pentahydroxycycloartane, 3-O-[α-L-rhamnopyranosyl-(1 → 2)-O-α-L-arabinopyranosyl-(1 → 2)-O-β-D-xylopyranosyl]-6-O-β-D-glucopyranosyl-3β,6α,16β,24(S),25-pentahydroxy cycloartane, 3-O-[α-L-arabinopyranosyl-(1 → 2)-O-3,4-diacetoxy-α-L-arabinopyranosyl]-6-O-β-D-glucopyranosyl-3β,6α,16β,24(S),25-pentahydroxycycloartane, 3-O-[α-L-arabinopyranosyl-(1 → 2)-O-3-acetoxy-α-L-arabinopyranosyl]-6-O-β-D-glucopyranosyl-3β,6α,16β,25-tetrahydroxy-20(R),24(S)-epoxycycloartane, 3-O-[α-L-arabinopyranosyl-(1 → 2)-O-β-D-xylopyranosyl]-6-O-β-D-glucopyranosyl-3β,6α,16β,24α-tetrahydroxy-20(R),25-epoxycycloartane, 3-O-[α-L-rhamnopyranosyl-(1 → 2)-O-α-L-arabinopyranosyl-(1 → 2)-O-β-D-xylopyranosyl]-6-O-β-D-glucopyranosyl-3β,6α,16β,24α-tetrahydroxy-20(R),25-epoxycycloartane by the extensive use of 1D- and 2D-NMR experiments along with ESIMS and HRMS analysis.The first four compounds are cyclocanthogenin and cycloastragenol glycosides, whereas the last two are based on cyclocephalogenin as aglycone, more unusual in the plant kingdom, so far reported only from Astragalus spp. 相似文献
28.
研究白眉蝮蛇(Agkistrodon halys ussuriensis)毒精氨酸酯酶的代谢动力学,为临床应用提供依据。125I标记的精氨酸酯酶对大鼠一侧颈静脉给药,不同时间从另一测颈静脉取血。5h后处死动物,取各组织、尿液、胆汁和粪便,对各样本的放射性进行测定并拟合时间-放射性关系曲线。代谢动力学拟合曲线符合一室模型,其中生物半衰期T1/2为55.9min,K值为0.0124min-1。125I标记的精氨酸酯酶在体内各组织广泛分布,但有血脑屏障存在,肝、肾和尿液中的放射性比其它组织要高很多,主要通过肝脏降解,肾脏排泄。 相似文献
29.
Microwave-accelerated energy-efficient esterification of free fatty acid with a heterogeneous catalyst 总被引:1,自引:0,他引:1
Kim D Choi J Kim GJ Seol SK Ha YC Vijayan M Jung S Kim BH Lee GD Park SS 《Bioresource technology》2011,102(3):3639-3641
This paper shows energy-efficiency of microwave-accelerated esterification of free fatty acid with a heterogeneous catalyst by net microwave power measurement. In the reaction condition of 5 wt% sulfated zirconia and 1:20 M ratio of oil to methanol at 60°C and atmospheric pressure, more than 90% conversion of the esterification was achieved in 20 min by microwave heating, while it took about 130 min by conventional heating. Electric energy consumption for the microwave heating in this accelerated esterification was only 67% of estimated minimum heat energy demand because of significantly reduced reaction time. 相似文献
30.