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41.
Shuai Ma Shuhui Sun Jiaming Li Yanling Fan Jing Qu Liang Sun Si Wang Yiyuan Zhang Shanshan Yang Zunpeng Liu Zeming Wu Sheng Zhang Qiaoran Wang Aihua Zheng Shuguang Duo Yang Yu Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Moshi Song Weiqi Zhang Guang-Hui Liu 《Cell research》2021,(4):415-432
Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Diseas... 相似文献
42.
XiaoLong Lin Zheng Liu LiPing Yan Xin Duan WenJun Bu XinHua Wang ChenGuang Zheng 《Ecology and evolution》2022,12(5)
Mitogenomes have been widely used for phylogenetic reconstruction of various Dipteran groups, but specifically for chironomid, they have not been carried out to resolve the relationships. Diamesinae (Diptera: Chironomidae) are important bioindicators for freshwater ecosystem monitoring, but its evolutionary history remains uncertain for lack of information. Here, coupled with one previously published and 30 new mitogenomes of Diamesinae, we carried out comparative mitogenomic analysis and phylogenetic analysis. Mitogenomes of Diamesinae were conserved in structure, and all genes arranged in the same order as the ancestral insect mitogenome. All protein‐coding genes in Diamesinae were under stronger purifying selection than those of other nonbiting midge species, which may exhibit signs of adaptation to life at cold living conditions. Phylogenetic analyses strongly supported the monophyly of Diamesinae, with Boreheptagyiini deeply nested within Diamesini. In addition, phylogenetic relationship of selected six genera was resolved, except Sympotthastia remained unstable. Our study revealed that the mitogenomes of Diamesinae are highly conserved, and they are practically useful for phylogenetic inference. 相似文献
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Weixiao Lei Zefu Wang Man Cao Hui Zhu Min Wang Yi Zou Yunchun Han Dandan Wang Zeyu Zheng Ying Li Bingbing Liu Dafu Ru 《DNA research》2022,29(3)
Sophora japonica is a medium-size deciduous tree belonging to Leguminosae family and famous for its high ecological, economic and medicinal value. Here, we reveal a draft genome of S. japonica, which was ∼511.49 Mb long (contig N50 size of 17.34 Mb) based on Illumina, Nanopore and Hi-C data. We reliably assembled 110 contigs into 14 chromosomes, representing 91.62% of the total genome, with an improved N50 size of 31.32 Mb based on Hi-C data. Further investigation identified 271.76 Mb (53.13%) of repetitive sequences and 31,000 protein-coding genes, of which 30,721 (99.1%) were functionally annotated. Phylogenetic analysis indicates that S. japonica separated from Arabidopsis thaliana and Glycine max ∼107.53 and 61.24 million years ago, respectively. We detected evidence of species-specific and common-legume whole-genome duplication events in S. japonica. We further found that multiple TF families (e.g. BBX and PAL) have expanded in S. japonica, which might have led to its enhanced tolerance to abiotic stress. In addition, S. japonica harbours more genes involved in the lignin and cellulose biosynthesis pathways than the other two species. Finally, population genomic analyses revealed no obvious differentiation among geographical groups and the effective population size continuously declined since 2 Ma. Our genomic data provide a powerful comparative framework to study the adaptation, evolution and active ingredients biosynthesis in S. japonica. More importantly, our high-quality S. japonica genome is important for elucidating the biosynthesis of its main bioactive components, and improving its production and/or processing. 相似文献
45.
A subsystem impactor test for pedestrian lower limb injury evaluation has been brought in China New Car Assessment Protocol(CNCAP).Concerning large anthropometr... 相似文献
46.
Nicola Pirastu Ciara McDonnell Eryk J. Grzeszkowiak Ninon Mounier Fumiaki Imamura Jordi Merino Felix R. Day Jie Zheng Nele Taba Maria Pina Concas Linda Repetto Katherine A. Kentistou Antonietta Robino Tnu Esko Peter K. Joshi Krista Fischer Ken K. Ong Tom R. Gaunt Zoltn Kutalik John R. B. Perry James F. Wilson 《PLoS genetics》2022,18(6)
Diet is considered as one of the most important modifiable factors influencing human health, but efforts to identify foods or dietary patterns associated with health outcomes often suffer from biases, confounding, and reverse causation. Applying Mendelian randomization in this context may provide evidence to strengthen causality in nutrition research. To this end, we first identified 283 genetic markers associated with dietary intake in 445,779 UK Biobank participants. We then converted these associations into direct genetic effects on food exposures by adjusting them for effects mediated via other traits. The SNPs which did not show evidence of mediation were then used for MR, assessing the association between genetically predicted food choices and other risk factors, health outcomes. We show that using all associated SNPs without omitting those which show evidence of mediation, leads to biases in downstream analyses (genetic correlations, causal inference), similar to those present in observational studies. However, MR analyses using SNPs which have only a direct effect on the exposure on food exposures provided unequivocal evidence of causal associations between specific eating patterns and obesity, blood lipid status, and several other risk factors and health outcomes. 相似文献
47.
Bingqing Xia Xurui Shen Yang He Xiaoyan Pan Feng-Liang Liu Yi Wang Feipu Yang Sui Fang Yan Wu Zilei Duan Xiaoli Zuo Zhuqing Xie Xiangrui Jiang Ling Xu Hao Chi Shuangqu Li Qian Meng Hu Zhou Yubo Zhou Xi Cheng Xiaoming Xin Lin Jin Hai-Lin Zhang Dan-Dan Yu Ming-Hua Li Xiao-Li Feng Jiekai Chen Hualiang Jiang Gengfu Xiao Yong-Tang Zheng Lei-Ke Zhang Jingshan Shen Jia Li Zhaobing Gao 《Cell research》2021,31(8):847-860
Cytokine storm and multi-organ failure are the main causes of SARS-CoV-2-related death. However, the origin of excessive damages caused by SARS-CoV-2 remains largely unknown. Here we show that the SARS-CoV-2 envelope (2-E) protein alone is able to cause acute respiratory distress syndrome (ARDS)-like damages in vitro and in vivo. 2-E proteins were found to form a type of pH-sensitive cation channels in bilayer lipid membranes. As observed in SARS-CoV-2-infected cells, heterologous expression of 2-E channels induced rapid cell death in various susceptible cell types and robust secretion of cytokines and chemokines in macrophages. Intravenous administration of purified 2-E protein into mice caused ARDS-like pathological damages in lung and spleen. A dominant negative mutation lowering 2-E channel activity attenuated cell death and SARS-CoV-2 production. Newly identified channel inhibitors exhibited potent anti-SARS-CoV-2 activity and excellent cell protective activity in vitro and these activities were positively correlated with inhibition of 2-E channel. Importantly, prophylactic and therapeutic administration of the channel inhibitor effectively reduced both the viral load and secretion of inflammation cytokines in lungs of SARS-CoV-2-infected transgenic mice expressing human angiotensin-converting enzyme 2 (hACE-2). Our study supports that 2-E is a promising drug target against SARS-CoV-2.Subject terms: Cell death, Molecular biology 相似文献
48.
两种麻疯树苗对盐胁迫的生理生态响应 总被引:7,自引:0,他引:7
研究两种不同基因型麻疯树苗(南油2、3号)在不同NaCl浓度下生理生态响应特征,并比较不同基因型麻疯树苗的耐盐差异性。结果表明:①用25、50 mmo.lL-1NaCl处理,南油2号全株干重与对照无显著差异,而南油3号全株干重比对照显著降低。用100 mmol.L-1l或以上浓度的NaCl处理,随着盐度增加,两种树苗全株干重皆比对照显著降低,且3号苗降低的幅度大于2号苗。②在用200 mmol.L-1或以下浓度的NaCl处理,南油2、3号叶片相对含水量(RWC)皆与对照无显著差异,而在用300 mmol.L-1NaCl处理,则分别比对照显著降低5%和8%。③用25、50 mmol.L-1NaCl处理,南油2号可溶性糖(SS)含量比对照显著降低,3号与对照无显著差异;用200、300 mmo.lL-1NaCl处理后,两者SS含量均比对照显著降低。同时,2号苗可溶性蛋白(SP)含量比对照显著增加,3号苗SP含量与对照无显著差异。④随着盐度增加,南油2号苗超氧化物歧化酶(SOD)活性先增加后降低。用300 mmol.L-1NaCl处理,比对照显著降低。随着盐度增加,3号苗的SOD活性递减,皆显著低于对照。用25、50 mmo.lL-1NaCl处理,两种树苗的过氧化物酶(POD)活性与对照无显著差异。随着盐度增加,2号苗的POD活性比对照显著增加,而3号苗比对照显著降低。用25、50 mmo.lL-1NaCl处理,两种树苗的过氧化氢酶(CAT)活性皆比对照显著增加,且随着盐度增加,其变化趋势如SOD活性。结果表明,麻疯树幼苗具有较好的耐盐性,且南油2号比南油3号具有更高的耐盐性,因为前者具有更高的保护酶活性、叶片保水能力和叶片SP含量。 相似文献
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