Use of moving aeration membrane bioreactor for the efficient production of tissue type plasminogen activator in serum free medium

A moving aeration-membrane (MAM) bioreactor was employed for the production of 2 μg/mL of tissue type Plasminogen Activator (tPA) in serum free medium from normal human fibroblast cells. This system could maintain high cell density for long periods of steady state conditions in perfusion cultivation. Under normal operating conditions, shear stress was as low as 0.65 dynes/cm² at the agitation speed of 80 rpm. Even though cell density gradually decreased with increasing agitation speed, tPA production increased linearly with increasing shear stress within a moderate range. This culture system allowed production of 2 μg tPA/mL while maintaining a high cell density of 1.0×10⁷ viable cells/mL.     

Light Funneling Profile During Enhanced Transmission Through a Subwavelength Metallic Slit

Plasmonics - The funneling profile of enhanced light transmission through a subwavelength slit in a perfect electric conductor is studied with finite-difference time-domain simulation. From the...     

Association of Common Variants in LOX with Keratoconus: A Meta-Analysis

Background

Several case-control studies have been performed to examine the association of genetic variants in lysyl oxidase (LOX) with keratoconus. However, the results remained inconclusive and great heterogeneity might exist across populations.

Method

A comprehensive literature search for studies that published up to June 25, 2015 was performed. Summary odds ratios (OR) and 95% confidence intervals (CI) of each single nucleotide polymorphism (SNP) were estimated with fixed effects model when I ²<50% in the test for heterogeneity or random effects model when I ²>50%. Publication bias was evaluated using funnel plots and Egger’s test.

Results

A total of four studies including 1,467 keratoconus cases and 4,490 controls were involved in this meta-analysis. SNPs rs2956540 and rs10519694 showed significant association with keratoconus, with ORs of 0.71 (95% CI: 0.63–0.80, P = 1.43E-08) and 0.77 (95% CI: 0.61–0.97, P = 0.026), respectively. In contrast, our study lacked sufficient evidences to support the association of rs1800449/rs2288393 with keratoconus across populations.

Conclusion

This meta-analysis suggested that two LOX variants, rs2956540 and rs10519694, may affect individual susceptibility to keratoconus, while distinct heterogeneity existed within this locus. Larger-scale and multi-ethnic genetic studies on keratoconus are required to further validate the results.     

汉坦病毒感染细胞整合素受体信号转导相关差异表达基因筛选

通过基因芯片技术观察汉坦病毒感染细胞后整合素受体信号转导相关基因的差异表达,以探讨汉坦病毒进入细胞的分子机制。用汉滩病毒76—118株感染原代人胚肺成纤维细胞,同时设立正常对照细胞,于感染后第6h、24h和96h同时抽提感染组与正常对照组细胞总RNA,利用荧光标记dUTP逆转录制备cDNA探针,与人类受体及信号转导类表达谱芯片杂交,并对Cy3、Cy5荧光信号做扫描分析。结果显示有37项基因出现差异表达,其中31项基因在感染后6h出现差异表达,13项下调、18项上调;但在感染后24h和96h分别只有5项和4项基因出现表达差异;而且整合素信号转导通路中的重要基因如一些蛋白激酶相关基因在感染6h表达增强,丝化增强子1在感染6h和96h表达下调,微管相关蛋白1A在感染6h表达上调、感染24h和96h表达下调。这些进一步说明汉坦病毒感染细胞与整合素有关。     

间斑寇蛛的生态学观察与实验

报道间斑寇蛛首次在南方大批量人工养殖成功所获得的行为生态学资料:养殖方法、捕食与食谱、活动规律、蜕皮与体色的关系;温度和湿度对间斑寇蛛活动能力、成活率、蜕皮率的影响;温度和食物对其若蛛生长发育的交互影响作用。间斑寇蛛生长发育的适宜温区是20～30℃,RH55%～75%;最适合温度为25℃,RH65%,苍蝇为较理想的食物。结合野外进行了种群密度的调查、初步分析了其种群分布与栖境生物群落的关系。     

A multiple RSU collaborative scheduling scheme for data services in vehicular ad hoc networks

As studies on vehicular ad hoc networks have been conducted actively in recent years, convenient and reliable services can be provided to vehicles through traffic information, surrounding information, and file sharing. To provide services for multiple requests, road side units (RSUs) should receive requests from vehicles and provide a scheduling scheme for data transfer according to priority. In this paper, we propose a new scheduling scheme by which multiple RSUs are connected through wired networks and data is transferred through the collaboration of RSUs. The proposed scheme transfers safety and non-safety data by employing a collaborative strategy of multiple RSUs as well as reducing the deadline miss ratio and average response time. When safety data is generated, data is transferred from the previous RSU in advance, while priority is assigned considering the deadline and reception rate. Since non-safety data is an on-demand data processed by user requests, the proposed scheme provides a method that reduces the deadline miss ratio upon loads generated in RSUs. To prove the superiority of the proposed scheme, we perform a performance evaluation in which the number and velocities of vehicles were changed. It is shown through the performance evaluation that the proposed scheme has better deadline miss ratios and faster response time than the existing schemes.