首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   9195篇
  免费   612篇
  国内免费   616篇
  10423篇
  2024年   24篇
  2023年   99篇
  2022年   240篇
  2021年   440篇
  2020年   305篇
  2019年   374篇
  2018年   362篇
  2017年   271篇
  2016年   345篇
  2015年   561篇
  2014年   663篇
  2013年   720篇
  2012年   803篇
  2011年   760篇
  2010年   442篇
  2009年   413篇
  2008年   468篇
  2007年   394篇
  2006年   362篇
  2005年   303篇
  2004年   258篇
  2003年   234篇
  2002年   185篇
  2001年   174篇
  2000年   151篇
  1999年   141篇
  1998年   105篇
  1997年   95篇
  1996年   83篇
  1995年   72篇
  1994年   82篇
  1993年   66篇
  1992年   71篇
  1991年   66篇
  1990年   66篇
  1989年   51篇
  1988年   32篇
  1987年   35篇
  1986年   22篇
  1985年   27篇
  1984年   10篇
  1983年   16篇
  1982年   8篇
  1981年   4篇
  1980年   6篇
  1979年   6篇
  1978年   2篇
  1977年   2篇
  1976年   2篇
  1972年   1篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
陈世雄  丁文柏 《蛇志》1999,11(1):23-24
目的寻求治疗结核包囊性胸腔积液的新方法。方法胸膜活检确诊为结核性胸腔积液并经B超或胸部X线证实有粘连包囊的病人15例,经胸腔内注入尿激酶每次10万单位,用60ml生理盐水稀释,每8~12h重复,连续5次,每次记录抽取胸水总量,并复查B超观察粘连包囊情况。对照组为单纯用抗结核治疗及间断抽胸水15例。结果实验组13例有效,总有效率86.7%。对照组2例有效,有效率13.3%,疗效明显优于对照组(P<0.05)。结论尿激酶胸腔内注入是治疗结核性粘连包囊性胸腔积液的一种安全可靠的新方法。  相似文献   
42.
43.
人Ⅳ型胶原的提纯及其抗血清制备   总被引:1,自引:0,他引:1  
采用胃蛋白酶限制性消化,NaCl分级盐析,还原和烷基化反应,纤维素离子交换层析从人胎盘组织分离纯化Ⅳ型胶原.经SDS-PAGE电泳鉴定符合Ⅳ型胶原α肽链电泳带.用纯化的Ⅳ型胶原免疫兔制备出高效价的特异抗血清.  相似文献   
44.
45.
鲸类繁殖生物学的研究概况   总被引:2,自引:0,他引:2  
生殖是生物繁殖自身的能力,生殖过程包括生殖细胞(精子与卵子)的生成与成熟、受精过程、妊娠、胎儿的发育、分娩等环节。    相似文献   
46.
The complete mitogenome of Haliotis iris, an economically important shellfish endemic to New Zealand, was sequenced for the first time. The mitogenome was 17,131?base pairs (bp) in length and contained 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and a control region. All 13 genes were initiated by the start codon ATG, except for nad5 (ATA). Two typical stop codons, TAA and TAG, were present. All of the tRNAs could be folded into typical cloverleaf secondary structures except tRNASer1 and tRNALys, which lacked a DHU stem and complete amino acid acceptor stem, respectively. The control region was 1132?bp in length and contained six AT tandem repeats. According to the gene order of the mitogenome, the 30 analysed Vetigastropoda species could be classified into three types—type I: over half of the studied species were very similar to the gastropod ancestral gene order, and the rearrangements occurred in five tRNAs; type II: eight species were found to be missing several tRNA genes; type III: Fissurellidae, Lepetodrilidae showed a large inverted fragment.  相似文献   
47.
ABCG2 is a member of the ATP binding cassette (ABC) transporters, which can pump a wide variety of endogenous and exogenous compounds out of cells. Widely expressed in stem cells, ABCG2 is also found to confer the side population phenotype and is recognized as a universal marker of stem cells. Although the precise physiological role of ABCG2 in stem cells is still unclear, existing data strongly suggest that ABCG2 plays an important role in promoting stem cell proliferation and the maintenance of the stem cell phenotype. In addition, ABCG2 is also found to be expressed in a number of cancer cells and appears to be a marker of cancer stem cells. Moreover, ABCG2 expression in tumors may contribute to their formation and progression. Thus, ABCG2 has potential applications in stem cell and tumor therapy.  相似文献   
48.
49.

Background

The one-step blending approach has been suggested for genomic prediction in dairy cattle. The core of this approach is to incorporate pedigree and phenotypic information of non-genotyped animals. The objective of this study was to investigate the improvement of the accuracy of genomic prediction using the one-step blending method in Chinese Holstein cattle.

Findings

Three methods, GBLUP (genomic best linear unbiased prediction), original one-step blending with a genomic relationship matrix, and adjusted one-step blending with an adjusted genomic relationship matrix, were compared with respect to the accuracy of genomic prediction for five milk production traits in Chinese Holstein. For the two one-step blending methods, de-regressed proofs of 17 509 non-genotyped cows, including 424 dams and 17 085 half-sisters of the validation cows, were incorporated in the prediction model. The results showed that, averaged over the five milk production traits, the one-step blending increased the accuracy of genomic prediction by about 0.12 compared to GBLUP. No further improvement in accuracies was obtained from the adjusted one-step blending over the original one-step blending in our situation. Improvements in accuracies obtained with both one-step blending methods were almost completely contributed by the non-genotyped dams.

Conclusions

Compared with GBLUP, the one-step blending approach can significantly improve the accuracy of genomic prediction for milk production traits in Chinese Holstein cattle. Thus, the one-step blending is a promising approach for practical genomic selection in Chinese Holstein cattle, where the reference population mainly consists of cows.  相似文献   
50.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号