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951.
通过生物素与亲和素-酶复合物系统或地高辛与抗地高辛-酶复合物系统可把酶间接标记到探针上.Renz等通过不同的化学方法直接把酶标记到探针上[1~3].耐热性碱性磷酸酯酶FD-TAP(thermostablealkalinephosphatase)具有耐... 相似文献
952.
EktacytometrymeasuringRBCdeformabilitywasfirstdevelopedbyBessisetal.in1975[1].Thistechniquehasbeenworldwideacceptednow.However,themeaningofdeformationindex(DI)measuredwiththismethodisstillworthstudying.ThetraditionalEktacytometryusessuspendingmediumofhighvi… 相似文献
953.
NifL, an antagonistic regulator of NifA interacting with NifA 总被引:3,自引:0,他引:3
954.
五种珍珠菜的核型研究 总被引:6,自引:2,他引:4
本文对5种珍珠菜亚属植物进行了染色体数目及核型研究。其中瓣珍珠菜2n=24=12m+8sm+4st(2SAT)、黑腺珍珠菜2n=22=2m+4sm+6st+10t、泽珍珠菜2n=24=14m+6sm(2SAT)+4st和小叶珍珠菜2n=48=34m+10sm+4st的染色体数目及核型为首次报道。中国九江产的红根草核型2n=24=20m+4sm(2SAT)与日本产的核型2n=24=18m(1SAT) 相似文献
955.
956.
957.
Li Xiao Yi Zhang Zhongqiu Liu Min Yang Lin Pu Dongfeng Pan 《Bioorganic & medicinal chemistry letters》2010,20(12):3515-3517
A neutrophil-binding peptide, cinnamoyl-F(D)LF(D)LF (cFLFLF), was labeled with the near infrared (NIR) fluorophore, Cyanine 7 (Cy7). This construct was modified with a polyethylene glycol (PEG, MW 3.4 kDa) moiety in order to increase its solubility and bioavailability to circulating neutrophils. A preliminary noninvasive fluorescence imaging of a mouse model of ear inflammation with the fluorescent probe is presented. 相似文献
958.
Hai-Yun Xiao Aaron Balog Ricardo M. Attar David Fairfax Linda B. Fleming Christian L. Holst Gregory S. Martin Lana M. Rossiter Jing Chen Mary-Ellen Cvjic Janet Dell-John Jieping Geng Marco M. Gottardis Wen-Ching Han Andrew Nation Mary Obermeier Cheryl A. Rizzo Liang Schweizer Thomas Spires Weifang Shan Gregory Vite 《Bioorganic & medicinal chemistry letters》2010,20(15):4491-4495
A novel series of 4-[3,5-dioxo-11-oxa-4,9-diazatricyclo[5.3.1.02,6]undec-4-yl]-2-trifluoromethyl-benzonitriles has been synthesized. The ability of these compounds to act as antagonists of the androgen receptor was investigated and several were found to have potent activity in vitro and in vivo. 相似文献
959.
Prapapan Teerawanichpan Albert J. Robertson Xiao Qiu 《Insect biochemistry and molecular biology》2010,40(9):641-649
Honey bees (Apis mellifera) are social insects which have remarkable complexity in communication pheromones. These chemical signals comprise a mixture of hydrocarbons, wax esters, fatty acids, aldehydes and alcohols. In this study, we detected several long chain aliphatic alcohols ranging from C18-C32 in honey bees and the level of these alcohols varied in each body segment. C18:0Alc and C20:0Alc are more pronounced in the head, whereas C22:0Alc to C32Alc are abundant in the abdomen. One of the cDNAs coding for a fatty acyl-CoA reductase (AmFAR1) involved in the synthesis of fatty alcohols was isolated and characterized. AmFAR1 was ubiquitously expressed in all body segments with the predominance in the head of honey bees. Heterologous expression of AmFAR1 in yeast revealed that AmFAR1 could convert a wide range of fatty acids (14:0–22:0) to their corresponding alcohols, with stearic acid 18:0 as the most preferred substrate. The substrate preference and the expression pattern of AmFAR1 were correlated with the level of total fatty alcohols in bees. Reconstitution of the wax biosynthetic pathway by heterologous expression of AmFAR1, together with Euglena wax synthase led to the high level production of medium to long chain wax monoesters in yeast. 相似文献
960.
Chenguang Lou Qiang Xiao Lavinia Brennan Mark E Light Nuria Vergara-Irigaray Elizabeth M. Atkinson Lindy M. Holden-Dye Keith R. Fox Tom Brown 《Bioorganic & medicinal chemistry》2010,18(17):6389-6397
2′-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases. 相似文献