全文获取类型
收费全文 | 76191篇 |
免费 | 5549篇 |
国内免费 | 4886篇 |
专业分类
86626篇 |
出版年
2024年 | 154篇 |
2023年 | 1035篇 |
2022年 | 2397篇 |
2021年 | 4073篇 |
2020年 | 2620篇 |
2019年 | 3232篇 |
2018年 | 3166篇 |
2017年 | 2298篇 |
2016年 | 3256篇 |
2015年 | 4811篇 |
2014年 | 5549篇 |
2013年 | 5984篇 |
2012年 | 7028篇 |
2011年 | 6158篇 |
2010年 | 3712篇 |
2009年 | 3337篇 |
2008年 | 3725篇 |
2007年 | 3356篇 |
2006年 | 2911篇 |
2005年 | 2381篇 |
2004年 | 1958篇 |
2003年 | 1655篇 |
2002年 | 1403篇 |
2001年 | 1231篇 |
2000年 | 1220篇 |
1999年 | 1126篇 |
1998年 | 663篇 |
1997年 | 656篇 |
1996年 | 667篇 |
1995年 | 617篇 |
1994年 | 543篇 |
1993年 | 376篇 |
1992年 | 569篇 |
1991年 | 435篇 |
1990年 | 406篇 |
1989年 | 283篇 |
1988年 | 245篇 |
1987年 | 234篇 |
1986年 | 166篇 |
1985年 | 193篇 |
1984年 | 109篇 |
1983年 | 117篇 |
1982年 | 71篇 |
1981年 | 58篇 |
1980年 | 37篇 |
1979年 | 61篇 |
1977年 | 30篇 |
1974年 | 38篇 |
1973年 | 34篇 |
1972年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
871.
Lifang Zou Guihua Tu Wei Xie Shiyao Wen Qiuyu Xie Shuangmei Liu Guilin Li Yun Gao Hong Xu Shouyu Wang Yun Xue Bing Wu Qiulan Lv Mofeng Ying Xi Zhang Shangdong Liang 《Purinergic signalling》2016,12(1):127-137
Adenosine triphosphate (ATP) acts on P2X receptors to initiate signal transmission. P2X7 receptors play a role in the pathophysiological process of myocardial ischemic injury. Long noncoding RNAs (lncRNAs) participate in numerous biological functions independent of protein translation. LncRNAs are implicated in nervous system diseases. This study investigated the effects of NONRATT021972 small interference RNA (siRNA) on the pathophysiologic processes mediated by P2X7 receptors in stellate ganglia (SG) after myocardial ischemic injury. Our results demonstrated that the expression of NONRATT021972 in SG was significantly higher in the myocardial ischemic (MI) group than in the control group. Treatment of MI rats with NONRATT021972 siRNA, the P2X7 antagonist brilliant blue G (BBG), or P2X7 siRNA improved the histology of injured ischemic cardiac tissues and decreased the elevated concentrations of serum myocardial enzymes, creatine kinase (CK), CK isoform MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) compared to the MI rats. NONRATT021972 siRNA, BBG, or P2X7 siRNA treatment in MI rats decreased the expression levels of P2X7 immunoreactivity, P2X7 messenger RNA (mRNA), and P2X7 protein, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK) in the SG compared to MI rats. NONRATT021972 siRNA treatment prevented the pathophysiologic processes mediated by P2X7 receptors in the SG after myocardial ischemic injury. 相似文献
872.
873.
874.
875.
876.
Sabrina Forveille Heng Zhou Allan Sauvat Lucillia Bezu Kevin Müller Peng Liu Laurence Zitvogel Gérard Pierron ?ystein Rekdal Oliver Kepp Guido Kroemer 《Cell cycle (Georgetown, Tex.)》2015,14(21):3506-3512
The oncolytic peptide LTX-315 has been designed for killing human cancer cells and turned out to stimulate anti-cancer immune responses when locally injected into tumors established in immunocompetent mice. Here, we investigated the question whether LTX-315 induces apoptosis or necrosis. Transmission electron microscopy or morphometric analysis of chromatin-stained tumor cells revealed that LTX-315 failed to induce apoptotic nuclear condensation and rather induced a necrotic phenotype. Accordingly, LTX-315 failed to stimulate the activation of caspase-3, and inhibition of caspases by means of Z-VAD-fmk was unable to reduce cell killing by LTX-315. In addition, 2 prominent inhibitors of regulated necrosis (necroptosis), namely, necrostatin-1 and cycosporin A, failed to reduce LTX-315-induced cell death. In conclusion, it appears that LTX-315 triggers unregulated necrosis, which may contribute to its pro-inflammatory and pro-immune effects. 相似文献
877.
878.
Dumont Henri J. Han Bo-Ping Guo Fei Fei Chen Hua Cheng Dan Liu Ping Xu Lei Sanoamuang La-Orsri Rietzler Arnola C. Xu Shaolin Vierstraete Andy Elias-Gutierrez Manuel 《Aquatic Ecology》2021,55(4):1207-1222
Aquatic Ecology - Diaphanosoma s.l., with 40+? described species, is the largest genus of the Sididae and the Ctenopoda, similar in many ways to the anomopod genus Daphnia. Here, we offer a c... 相似文献
879.
Kaina Wang Yani Liu Yan Shi Mingzhu Yan Thamaraiselvan Rengarajan Xin Feng 《Saudi Journal of Biological Sciences》2021,28(8):4247-4254
BackgroundChronic epilepsy is a multifaceted common brain disorder with manifold underlying factors. Epilepsy affects around 70 million peoples worldwide. Amomum tsaoko is a perennial herbaceous plant that is extensively cultivated in many provinces of China reported to exert immense biological activities.ObjectiveThis research work was aimed to reveal the therapeutic actions of ethanolic extract of A.tsaoko fruits (EE-ATF) against the pentylenetetrazol (PTZ)-provoked convulsive seizures in the mice.MethodologyThe convulsive seizures were provoked to the animals via administering 70 mg/kg of PTZ through intraperitoneally to trigger the convulsive seizures then treated with the EE-ATF at 50, 75, and 100 mg/kg orally 30 min prior to PTZ challenge. After the 30 min of PTZ challenge, animals closely monitored for signs of convulsion, generalized clonic and tonic convulsion durations, and mortality. A sub-convulsive dose 35 mg/kg of PTZ was used to provoke the kindling and seizure stages were examined using standard method. The levels of dopamine, GABA, glutamate, and Na + K + ATPase and Ca + ATPase activities in the brain tissues were studied using marker specific assay kits. The oxidative stress and antioxidant markers studied using standard methods. The mRNA expressions of COX-2, TNF-α, NF-κB, TLR-4, and IL-1β in the brain tissues were studied using RT-PCR analysis. The brain tissues were examined histologically.ResultsEE-ATF treatment remarkably decreased the onset and duration of convulsion and suppressed the seizure severity and mortality in the PTZ animals. EE-ATF treatment appreciably ameliorated the PTZ triggered modifications in the GABA, glutamate, dopamine levels and Ca + 2ATPase and Na + K + ATPase activities in the brain tissues. EE-ATF suppressed the mRNA expressions of NF-κB, IL-1β, TLR-4, TNF-α, and COX-2. The status of antioxidants were elevated by the EE-ATF. Histological findings also demonstrated the curative actions of EE-ATF.ConclusionOur findings evidenced that the EE-ATF substantially ameliorated the PTZ-provoked convulsive seizures in the mice. 相似文献
880.