Nguyen Thi Kim Oanh  Ho-Soo Lee  Yong-Hyun Kim  Sunwoo Min  Yeon-Ji Park  June Heo  Yong-Yea Park  Won-Chung Lim  Hyeseong Cho 《Nucleic acids research》2022,50(16):9247

Cells are constantly challenged by genotoxic stresses that can lead to genome instability. The integrity of the nuclear genome is preserved by the DNA damage response (DDR) and repair. Additionally, these stresses can induce mitochondria to transiently hyperfuse; however, it remains unclear whether canonical DDR is linked to these mitochondrial morphological changes. Here, we report that the abolition of mitochondrial fusion causes a substantial defect in the ATM-mediated DDR signaling. This deficiency is overcome by the restoration of mitochondria fusion. In cells with fragmented mitochondria, genotoxic stress-induced activation of JNK and its translocation to DNA lesion are lost. Importantly, the mitochondrial fusion machinery of MFN1/MFN2 associates with Sab (SH3BP5) and JNK, and these interactions are indispensable for the Sab-mediated activation of JNK and the ATM-mediated DDR signaling. Accordingly, the formation of BRCA1 and 53BP1 foci, as well as homology and end-joining repair are impaired in cells with fragmented mitochondria. Together, these data show that mitochondrial fusion-dependent JNK signaling is essential for the DDR, providing vital insight into the integration of nuclear and cytoplasmic stress signals.  相似文献   

    

Cho  Bumrae  Lee  Eun-Jin  Ahn  Sun Mi  Kim  Ghangyong  Lee  Sang Hoon  Ji  Dal-Young  Kang  Jung-Taek 《Transgenic research》2019,28(5-6):549-559

Transgenic Research - Islet xenotransplantation is a promising treatment for type I diabetes. Numerous studies of islet xenotransplantation have used pig-to-nonhuman primate transplantation models....  相似文献   

    

Vadir López-Salmerón  Hyunwoo Cho  Nina Tonn  Thomas Greb 《Current biology : CB》2019,29(5):R173-R181

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Bashir  Khawaja Muhammad Imran  Mansoor  Sana  Kim  Na-Ri  Grohmann  Florian Rolando  Shah  Abid Ali  Cho  Man-Gi 《Annals of microbiology》2019,69(4):353-368

The present study aimed to investigate the effects of organic carbon sources, cultivation methods, and environmental factors on growth and lipid content of Pavlova lutheri for biodiesel production. In the 250-mL flask bioreactors, P. lutheri was cultivated in the modified artificial seawater (ASW) medium containing glucose, glycerol, sodium acetate, or sucrose as an organic carbon substrate. The effects of different growth conditions (phototrophic, mixotrophic, and heterotrophic) and environmental factors such as photoperiod, light intensity, and salinity were evaluated. Growth of P. lutheri was inhibited under heterotrophy but was enhanced in mixotrophy as compared to phototrophy. Biomass and lipid content of P. lutheri were significantly (p < 0.05) affected by changing photoperiod, light intensity, and salinity. Higher biomass concentration and lipid content were observed at a light intensity of 100 ± 2 μmol photons m−2 s−1, 18 h photoperiod, and 30% salinity, in a modified ASW medium supplemented with 10 mmol sucrose. An increase in biomass concentration from 320 ± 25.53 to 1106 ± 18.52 mg L−1 and high lipid content of 31.11 ± 1.65% (w/w) were observed with the optimized culture conditions, demonstrating a significant (p < 0.05) enhancement in biomass and lipid content due to the improved culture conditions. The present study emphasizes the possible use of sucrose for biomass and lipid production with P. lutheri under the optimized culture conditions. Using low-cost and relatively easy accessible feedstock such as sucrose would be a valuable alternative for growing microalgae with enhanced lipid content.  相似文献   

    

Li  Yingnan  Song  Youngkeun  Cho  Donggil  Han  Zhen 《Landscape and Ecological Engineering》2019,15(3):265-276

Landscape and Ecological Engineering - Microclimates in urban areas arise through the interaction between prevailing weather conditions and landscape design parameters. A well-arranged landscape...  相似文献   

    

Emily Ling-Lin Pai  Daniel Vogt  Alexandra Clemente-Perez  Gabriel L. McKinsey  Frances S. Cho  Jia Sheng Hu  Matt Wimer  Anirban Paul  Siavash Fazel Darbandi  Ramon Pla  Tomasz J. Nowakowski  Lisa V. Goodrich  Jeanne T. Paz  John L.R. Rubenstein 《Cell reports》2019,26(5):1157-1173.e5

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Subbroto Kumar Saha  S. M. Riazul Islam  Tripti Saha  Afsana Nishat  Polash Kumar Biswas  Minchan Gil  Lewis Nkenyereye  Shaker El-Sappagh  Md. Saiful Islam  Ssang-Goo Cho 《BMB reports》2021,54(10):497

EGR1 (early growth response 1) is dysregulated in many cancers and exhibits both tumor suppressor and promoter activities, making it an appealing target for cancer therapy. Here, we used a systematic multiomics analysis to review the expression of EGR1 and its role in regulating clinical outcomes in breast cancer (BC). EGR1 expression, its promoter methylation, and protein expression pattern were assessed using various publicly available tools. COSMIC-based somatic mutations and cBioPortal-based copy number alterations were analyzed, and the prognostic roles of EGR1 in BC were determined using Prognoscan and Kaplan-Meier Plotter. We also used bc-GenEx-Miner to investigate the EGR1 co-expression profile. EGR1 was more often downregulated in BC tissues than in normal breast tissue, and its knockdown was positively correlated with poor survival. Low EGR1 expression levels were also associated with increased risk of ER+, PR+, and HER2-BCs. High positive correlations were observed among EGR1, DUSP1, FOS, FOSB, CYR61, and JUN mRNA expression in BC tissue. This systematic review suggested that EGR1 expression may serve as a prognostic marker for BC patients and that clinicopathological parameters influence its prognostic utility. In addition to EGR1, DUSP1, FOS, FOSB, CYR61, and JUN can jointly be considered prognostic indicators for BC.  相似文献   

    

Somin Lee  Habin Kang  Dohyun Park  James Yu  Seung Kwon Koh  Duck Cho  Da-Hyun Kim  Kyung-Sun Kang  Noo Li Jeon 《Advanced Biosystems》2021,5(2):2170021

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Seong Gwan Yu  Na Hyun Cho  Jong Hum Kim  Tae Rin Oh  Woo Taek Kim 《植物学报(英文版)》2021,63(3):431-437

Drought stress has detrimental effects on plants. Although the abscisic acid (ABA)‐mediated drought response is well established, defensive mechanisms to cope with dehydration‐induced proteotoxicity have been rarely studied. DRR1 was identified as an Arabidopsis drought‐induced gene encoding an ER‐localized RING‐type E3 Ub ligase. Suppression of DRR1 markedly reduced tolerance to drought and proteotoxic stress without altering ABA‐mediated germination and stomatal movement. Proteotoxicity‐ and dehydration‐induced insoluble ubiquitinated protein accumulation was more obvious in DRR1 loss‐of‐function plants than in wild‐type plants. These results suggest that DRR1 is involved in an ABA‐independent drought stress response possibly through the mitigation of dehydration‐induced proteotoxic stress.  相似文献   

    

Won Yong Jung  Sang Sook Lee  Chul Wook Kim  Hyun-Soon Kim  Sung Ran Min  Jae Sun Moon  Suk-Yoon Kwon  Jae-Heung Jeon  Hye Sun Cho 《PloS one》2014,9(11)

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