全文获取类型
收费全文 | 160415篇 |
免费 | 8423篇 |
国内免费 | 4966篇 |
专业分类
173804篇 |
出版年
2023年 | 911篇 |
2022年 | 2080篇 |
2021年 | 3612篇 |
2020年 | 2372篇 |
2019年 | 2851篇 |
2018年 | 3726篇 |
2017年 | 3052篇 |
2016年 | 5592篇 |
2015年 | 10600篇 |
2014年 | 10907篇 |
2013年 | 11317篇 |
2012年 | 11426篇 |
2011年 | 8168篇 |
2010年 | 5795篇 |
2009年 | 5327篇 |
2008年 | 4587篇 |
2007年 | 4077篇 |
2006年 | 3645篇 |
2005年 | 9129篇 |
2004年 | 7468篇 |
2003年 | 5398篇 |
2002年 | 2762篇 |
2001年 | 2630篇 |
2000年 | 1872篇 |
1999年 | 2893篇 |
1998年 | 1159篇 |
1997年 | 956篇 |
1996年 | 907篇 |
1995年 | 885篇 |
1994年 | 846篇 |
1992年 | 2729篇 |
1991年 | 2665篇 |
1990年 | 2665篇 |
1989年 | 2523篇 |
1988年 | 2369篇 |
1987年 | 2170篇 |
1986年 | 1957篇 |
1985年 | 1948篇 |
1984年 | 1307篇 |
1983年 | 1031篇 |
1979年 | 1220篇 |
1978年 | 843篇 |
1975年 | 983篇 |
1974年 | 1110篇 |
1973年 | 1080篇 |
1972年 | 1014篇 |
1971年 | 971篇 |
1970年 | 864篇 |
1969年 | 855篇 |
1968年 | 767篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
151.
152.
Our studies aimed at determining a loss of active heparin from the peritoneal cavity after its intraperitoneal administration (250 JU/l of dialysis fluid) in 16 patients treated because of the end-stage renal failure with intermittent peritoneal dialysis and at comparing heparin influx clearance with that of glucose. It has been shown that heparin used in this dose loses 60-70% of its activity after 20-minute equilibration of dialysis fluid in the peritoneal cavity. Heparin influx clearance is higher than that of glucose but it depends on utilization of heparin in peritoneal cavity rather than on its penetration to the blood circulation. 相似文献
153.
154.
Mycoplasmas exhibit a novel, substrate-dependent gliding motility that is driven by ∼400 “leg” proteins. The legs interact with the substrate and transmit the forces generated by an assembly of ATPase motors. The velocity of the cell increases linearly by nearly 10-fold over a narrow temperature range of 10-40°C. This corresponds to an Arrhenius factor that decreases from ∼45 kBT at 10°C to ∼10 kBT at 40°C. On the other hand, load-velocity curves at different temperatures extrapolate to nearly the same stall force, suggesting a temperature-insensitive force-generation mechanism near stall. In this article, we propose a leg-substrate interaction mechanism that explains the intriguing temperature sensitivity of this motility. The large Arrhenius factor at low temperature comes about from the addition of many smaller energy barriers arising from many substrate-binding sites at the distal end of the leg protein. The Arrhenius dependence attenuates at high temperature due to two factors: 1), the reduced effective multiplicity of energy barriers intrinsic to the multiple-site binding mechanism; and 2), the temperature-sensitive weakly facilitated leg release that curtails the power stroke. The model suggests an explanation for the similar steep, sub-Arrhenius temperature-velocity curves observed in many molecular motors, such as kinesin and myosin, wherein the temperature behavior is dominated not by the catalytic biochemistry, but by the motor-substrate interaction. 相似文献
155.
156.
Transfer RNAs, isolated from Escherichia coli F cells infected with T5 bacteriophage, were charged with radioactive amino acids and used in RNA-DNA hybridization studies to detect and locate T5 tRNA cistrons in the T5 DNA chromosome. Hybridization of 14 3H-aminoacyl-tRNA species, including purified T5 [35S]Met-tRNAm and [35S]Met-tRNAf, to the separated strands of T5+ DNA indicates that most, if not all, of the T5 tRNAs are transcribed from the continuous heavy strand of T5 DNA. Heteroduplex mapping of eight mutant T5 DNA deletions has enabled us to locate and determine the size of these deleted segments. By correlating this information with the presence and absence of specific tDNA sequences in these mutants, as determined by tRNA-DNA hybridization, we were able to define the physical limits of four tDNA-containing loci along the T5 DNA molecule. A physical map for 15 tRNA species examined indicates that the structural genes for these tRNAs are clustered within a segment length of T5 DNA that represents approximately 11.2% of the total wild type T5 DNA. The existence of the deletion mutants indicates that T5 tRNAs are dispensable for T5 replication under the growth conditions and for the host employed. 相似文献
157.
158.
159.
160.
The anatomical basis for the application of neurovascular pedicled muscle transfers of the digastric and stylohyoid muscles in the treatment of velopharynx incompetence is described. The fact that the neurovascular pedicle is located in the cranial third of the muscle bellies provides the safety of the operative procedure. The muscles have to be dissected with respect to that. The direction in which the transferred muscles pull is described. The muscle transposition is combined with the classic Wardill-Kilner operation to lengthen the soft palate. The transferred muscles have to avoid scar contraction and shortening of the soft palate and to gain a muscular function of the soft palate. The clinical use is justified in rare cases as demonstrated in one case. 相似文献