全文获取类型
收费全文 | 21471篇 |
免费 | 1642篇 |
国内免费 | 1605篇 |
专业分类
24718篇 |
出版年
2024年 | 56篇 |
2023年 | 252篇 |
2022年 | 662篇 |
2021年 | 1163篇 |
2020年 | 764篇 |
2019年 | 935篇 |
2018年 | 905篇 |
2017年 | 675篇 |
2016年 | 948篇 |
2015年 | 1300篇 |
2014年 | 1572篇 |
2013年 | 1724篇 |
2012年 | 1893篇 |
2011年 | 1775篇 |
2010年 | 1094篇 |
2009年 | 1002篇 |
2008年 | 1139篇 |
2007年 | 1008篇 |
2006年 | 865篇 |
2005年 | 741篇 |
2004年 | 581篇 |
2003年 | 551篇 |
2002年 | 450篇 |
2001年 | 326篇 |
2000年 | 326篇 |
1999年 | 325篇 |
1998年 | 189篇 |
1997年 | 170篇 |
1996年 | 181篇 |
1995年 | 177篇 |
1994年 | 158篇 |
1993年 | 116篇 |
1992年 | 158篇 |
1991年 | 113篇 |
1990年 | 110篇 |
1989年 | 77篇 |
1988年 | 54篇 |
1987年 | 46篇 |
1986年 | 36篇 |
1985年 | 35篇 |
1984年 | 17篇 |
1983年 | 15篇 |
1982年 | 16篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1975年 | 2篇 |
1966年 | 1篇 |
1950年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 8 毫秒
901.
902.
Yaou Shen Zhou Jiang Sifen Lu Haijian Lin Shibin Gao Huanwei Peng Guangsheng Yuan Li Liu Zhiming Zhang Maojun Zhao Tingzhao Rong Guangtang Pan 《Biochemical and biophysical research communications》2013
Genetic transformation of maize is highly dependent on the development of embryonic calli from the dedifferentiated immature embryo. To better understand the regulatory mechanism of immature embryo dedifferentiation, we generated four small RNA and degradome libraries from samples representing the major stages of dedifferentiation. More than 186 million raw reads of small RNA and degradome sequence data were generated. We detected 102 known miRNAs belonging to 23 miRNA families. In total, we identified 51, 70 and 63 differentially expressed miRNAs (DEMs) in the stage I, II, III samples, respectively, compared to the control. However, only 6 miRNAs were continually up-regulated by more than fivefold throughout the process of dedifferentiation. A total of 87 genes were identified as the targets of 21 DEM families. This group of targets was enriched in members of four significant pathways including plant hormone signal transduction, antigen processing and presentation, ECM-receptor interaction, and alpha-linolenic acid metabolism. The hormone signal transduction pathway appeared to be particularly significant, involving 21 of the targets. While the targets of the most significant DEMs have been proved to play essential roles in cell dedifferentiation. Our results provide important information regarding the regulatory networks that control immature embryo dedifferentiation in maize. 相似文献
903.
Ke Xing Guang-Kai Bian Sheng Qin Hans-Peter Klenk Bo Yuan Yue-Ji Zhang Wen-Jun Li Ji-Hong Jiang 《Antonie van Leeuwenhoek》2013,104(6):1245-1245
904.
905.
906.
Different groups including ours have shown that curcumin induces melanoma cell apoptosis, here we focused the role of mammalian Sterile 20-like kinase 1 (MST1) in it. We observed that curcumin activated MST1-dependent apoptosis in cultured melanoma cells. MST1 silencing by RNA interference (RNAi) suppressed curcumin-induced cell apoptosis, while MST1 over-expressing increased curcumin sensitivity. Meanwhile, curcumin induced reactive oxygen species (ROS) production in melanoma cells, and the ROS scavenger, N-acetyl-cysteine (NAC), almost blocked MST1 activation to suggest that ROS might be required for MST1 activation by curcumin. c-Jun N-terminal protein kinase (JNK) activation by curcumin was dependent on MST1, since MST1 inhibition by RNAi or NAC largely inhibited curcumin-induced JNK activation. Further, curcumin induced Foxo3 nuclear translocation and Bim-1 (Foxo3 target gene) expression in melanoma cells, such an effect by curcumin was inhibited by MST1 RNAi. In conclusion, we suggested that MST1 activation by curcumin mediates JNK activation, Foxo3a nuclear translocation and apoptosis in melanoma cells. 相似文献
907.
Ling-Xue Jiang Long-Guo Jin Yong Guo Bo Tao Li-Juan Qiu 《Biochemical and biophysical research communications》2013
Glyphosate is a broad spectrum, non-selective herbicide which has been widely used for weed control. Much work has focused on elucidating the high accumulation of glyphosate in shoot apical bud (shoot apex). However, to date little is known about the molecular mechanisms of the sensitivity of shoot apical bud to glyphosate. Global gene expression profiling of the soybean apical bud response to glyphosate treatment was performed in this study. The results revealed that the glyphosate inhibited tryptophan biosynthesis of the shikimic acid pathway in the soybean apical bud, which was the target site of glyphosate. Glyphosate inhibited the expression of most of the target herbicide site genes. The promoter sequence analysis of key target genes revealed that light responsive elements were important regulators in glyphosate induction. These results will facilitate further studies of cloning genes and molecular mechanisms of glyphosate on soybean shoot apical bud. 相似文献
908.
909.
Shan Cheng Yang Li Ying Yang Duiping Feng Longyan Yang Qian Ma Shuai Zheng Ran Meng Shuhui Wang Songlin Wang Wen G. Jiang Junqi He 《FEBS letters》2013
Na+/H+ exchanger regulatory factor 1 (NHERF1) is a scaffold protein known to interact with a number of cancer-related proteins. nherf1 Mutations (K172N and D301V) were recently identified in breast cancer cells. To investigate the functional properties of NHERF1, wild-type and cancer-derived nherf1 mutations were stably expressed in SKMES-1 cells respectively. NHERF1-wt overexpression suppressed the cellular malignant phenotypes, including proliferation, migration, and invasion. nherf1 Mutations (K172N and D301V) caused complete or partial loss of NHERF1 functions by affecting the PTEN/NHERF1/PDGFRβ complex formation, inactivating NHERF1 inhibition of PDGF-induced AKT and ERK activation, and attenuating the tumor-suppressor effects of NHERF1-wt. These results further demonstrated the functional consequences of breast cancer-derived nherf1 mutations (K172N and D301V), and suggested the causal role of NHERF1 in tumor development and progression. 相似文献
910.