全文获取类型
收费全文 | 25941篇 |
免费 | 2222篇 |
国内免费 | 1645篇 |
专业分类
29808篇 |
出版年
2024年 | 62篇 |
2023年 | 275篇 |
2022年 | 702篇 |
2021年 | 1065篇 |
2020年 | 777篇 |
2019年 | 957篇 |
2018年 | 1019篇 |
2017年 | 747篇 |
2016年 | 1094篇 |
2015年 | 1663篇 |
2014年 | 1956篇 |
2013年 | 2010篇 |
2012年 | 2400篇 |
2011年 | 2257篇 |
2010年 | 1341篇 |
2009年 | 1296篇 |
2008年 | 1496篇 |
2007年 | 1336篇 |
2006年 | 1192篇 |
2005年 | 1013篇 |
2004年 | 968篇 |
2003年 | 797篇 |
2002年 | 658篇 |
2001年 | 441篇 |
2000年 | 360篇 |
1999年 | 307篇 |
1998年 | 219篇 |
1997年 | 187篇 |
1996年 | 185篇 |
1995年 | 157篇 |
1994年 | 142篇 |
1993年 | 87篇 |
1992年 | 114篇 |
1991年 | 90篇 |
1990年 | 97篇 |
1989年 | 76篇 |
1988年 | 52篇 |
1987年 | 52篇 |
1986年 | 36篇 |
1985年 | 30篇 |
1984年 | 38篇 |
1983年 | 18篇 |
1982年 | 12篇 |
1981年 | 8篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1975年 | 2篇 |
1967年 | 3篇 |
1964年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
951.
记述了广西跳蛛一新种:陈氏八木蛛Yaginumanis cheni sp.nov.。模式标本保存于中国科学院动物研究所,量度单位nm。 相似文献
952.
953.
Xie G Uttamchandani M Chen GY Bu X Lin SS Wong KM Yan W Yao SQ Guo Z 《Bioorganic & medicinal chemistry letters》2002,12(6):989-992
Apparent kinetic constants k(cat) and K(m) were determined for tyrocidine thioesterase (TycC TE) using randomized peptide N-acetylcysteamine thioesters as substrate analogues. The enzyme has been found to be adequately active for the synthesis of positional-scanning libraries for novel antibiotic screening with reduced k(cat)/K(m) in the range of 2 to 82 folds lower than that of the wild-type sequence 相似文献
954.
Jae Young Choi Myung-Pyo Jung Hong-Hyun Park Xue Ying Tao Byung Rae Jin Yeon Ho Je 《Journal of Asia》2013,16(1):75-80
To improve the insecticidal activity of Autographa californica nucleopolyhedrovirus (AcMNPV), using co-expression of Bacillus thuringiensis crystal protein and a Kunitz-type toxin isolated from bumblebee Bombus ignitus venom, a recombinant AcMNPV, ApPolh5-3006BiKTI, expressing Bi-KTI under the control of early promoter from Cotesia plutellae bracovirus (CpBV) was constructed. In this recombinant virus, B. thuringiensis cry1-5 crystal protein gene was introduced into the genome by the fusion of polyhedrin-cry1-5 under the control of polyhedrin gene promoter. RT-PCR analysis indicated that both Bi-KTI and polyhedrin-cry1-5 fusion protein were successfully expressed from the infected cells. In addition, SDS-PAGE revealed that polyhedrin-cry1-5 fusion protein expressed by recombinant viruses was occluded into the polyhedra. ApPolh5-3006BiKTI showed an improved insecticidal activity against larvae of Plutella xylostella and Spodoptera exigua. At low dosage rates, it was more effective against S. exigua than on P. xylostella, but more rapid insecticidal activity was shown in P. xylostella. These results strongly suggest that co-expression of Bt toxin and Kunitz-type toxins could be successfully applied to improve the insecticidal activity of baculoviruses. 相似文献
955.
Jin Wang Ping Liu Mary F.H. She Saeid Nahavandi Abbas Kouzani 《Biomedical signal processing and control》2013,8(6):634-644
Automatic analysis of biomedical time series such as electroencephalogram (EEG) and electrocardiographic (ECG) signals has attracted great interest in the community of biomedical engineering due to its important applications in medicine. In this work, a simple yet effective bag-of-words representation that is originally developed for text document analysis is extended for biomedical time series representation. In particular, similar to the bag-of-words model used in text document domain, the proposed method treats a time series as a text document and extracts local segments from the time series as words. The biomedical time series is then represented as a histogram of codewords, each entry of which is the count of a codeword appeared in the time series. Although the temporal order of the local segments is ignored, the bag-of-words representation is able to capture high-level structural information because both local and global structural information are well utilized. The performance of the bag-of-words model is validated on three datasets extracted from real EEG and ECG signals. The experimental results demonstrate that the proposed method is not only insensitive to parameters of the bag-of-words model such as local segment length and codebook size, but also robust to noise. 相似文献
956.
The functional deficiencies of bone marrow-derived mesenchymal stem cells (MSCs) may contribute to the aging process and age-related diseases, such as osteoporosis. Although it has been reported that microRNAs (miRNAs) played an important role in mechanisms of gene regulation of aging, and their expression profiles in MSCs osteogenic differentiation were established in recent years, but it is still elusive for the dynamic patterns of miRNAs in aging process. Importantly, the miRNAs in aged bone tissue had not been yet reported so far. Here, we combined high through-put sequencing with computational techniques to detect miRNAs dynamics in MSCs and bone tissue of age-related osteoporosis. Among the detected miRNAs, 59 identified miRNAs in MSCs and 159 in bone showed significantly differential expressions. And more importantly, there existed 8 up-regulated and 30 down-regulated miRNAs in both MSCs and bone during the aging process, with the majority having a trend of down-regulation. Furthermore, after target prediction and KEGG pathway analysis, we found that their targeted genes were significantly enriched in pathways in cancer, which are complex genetic networks, comprise of a number of age-related pathways. These results strongly suggest that these analyzed miRNAs may be negatively involved in age-related osteoporosis, given that most of them showed a decreased expression, which could lay a good foundation for further functional analysis of these miRNAs in age-related osteoporosis. 相似文献
957.
Amir Reza Hajrasouliha Guomin Jiang Qingxian Lu Huayi Lu Henry J. Kaplan Huang-Ge Zhang Hui Shao 《The Journal of biological chemistry》2013,288(39):28058-28067
Exosomes released from different types of host cells have different biological effects. We report that exosomes released from retinal astroglial cells (RACs) suppress retinal vessel leakage and inhibit choroidal neovascularization (CNV) in a laser-induced CNV model, whereas exosomes released from retinal pigmental epithelium do not. RAC exosomes inhibit the migration of macrophages and the tubule forming of mouse retinal microvascular endothelial cells. Further, we analyzed antiangiogenic components in RAC exosomes using an angiogenesis array kit and detected several endogenous inhibitors of angiogenesis exclusively present in RAC exosomes, such as endostatin. Moreover, blockade of matrix metalloproteinases in the cleavage of collagen XVIII to form endostatin using FN-439 reverses RAC exosome-mediated retinal vessel leakage. This study demonstrates that exosomes released from retinal tissue cells have different angiogenic effects, with exosomes from RACs containing antiangiogenic components that might protect the eye from angiogenesis and maintain its functional integrity. In addition, by identifying additional components and their functions of RAC exosomes, we might improve the antiangiogenic therapy for CNV in age-related macular degeneration and diabetic retinopathy. 相似文献
958.
Krukenberg tumor originated from stomach in female patients is common in clinical practice, but it is still uncertain whether surgical resection of ovarian metastases could improve the outcome. Some studies suggested that a certain group of patients could benefit from the resection of ovarian metastases. However, conclusions were different between studies and there was no data to illustrate if certain molecular markers were associated with patients’ survival. In this study, we analyzed the effects of resection of ovarian metastases, and investigated prognostic factors in 133 patients with ovarian metastases originated from stomach. Furthermore, we examined the expression of some cancer stem cells (CSCs) markers or related molecules in 64 ovarian metastases specimens and analyzed the correlation between these molecules and patients’ survival. We found that the median overall survival (mOS) of all 133 patients was 16 months, and “gastrectomy” and “without ascites” were two independent prognostic factors associated with longer survival. The mOS of the patients with gastrectomy was longer than that of patients had not undergone gastrectomy (19 vs. 9 months, p = 0.048). Patients without ascites survived longer than those with ascites (mOS: 21 vs. 13 months, p = 0.008). We also found that Sox2, CD44 or CD133 positive expression in ovarian metastases were risk factors correlated with poor survival, and Sox2 expression was an independent prognostic indicator. These results suggested that ovarian metastasectomy might help to prolong the survivor of some patients with Krukenberg tumor originated from stomach. Patients without ascites, and with resected or resectable primary gastric cancer lesion could get benefit from and be potential candidate for surgical treatment. The expression of Sox2 might serve as a prognostic indicator for predicting patients’ survival and be helpful for selecting patients in future. 相似文献
959.
Sung-Tae Hong Yan Jin Khikmat Anvarov Abdukhakim Khadjibaev Samin Hong Yusufjon Ahmedov Utkir Otaboev 《The Korean journal of parasitology》2013,51(3):383-385
Uzbekistan is endemic of cystic echinococcosis (CE). In order to estimate endemicity of CE, we collected data from emergency surgery due to CE in 2002-2010 and also investigated the prevalence of hydatid cysts in the liver and lungs of sheep at an abattoir in Uzbekistan from July 2009 to June 2010. In 14 emergency hospitals, 8,014 patients received surgical removal or drainage of CE during 2002-2010, and 2,966 patients were found in 2010. A total of 22,959 sheep were grossly examined of their liver and lungs, and 479 (2.1%) and 340 (1.5%) of them were positive for the cyst in the liver and lungs, respectively. Echinococcus granulosus is actively transmitted both to humans and sheep, and CE is a zoonotic disease of public health priority in Uzbekistan. 相似文献
960.