Erratum to: MYH7B variants cause hypertrophic cardiomyopathy by activating the CaMK-signaling pathway

Science China Life Sciences -     

Responses of morphological and physiological traits to herbivory by snails of three invasive and native submerged plants

3种入侵和本地沉水植物形态和生理性状对螺类牧食的响应 沉水植物水盾草(Cabomba caroliniana)已成为中国太湖流域的优势入侵水生植物。与外来物种的原产地环境相比,引入地新环境中存在的专食性天敌较少。外来物种可能会逃避其原产地环境中的天敌牧食,又因为它们的适口性相对较差,从而导致在引入地外来物种通常比本地物种遭受的牧食者影响更低(天敌逃逸假说)。本研究的目的是比较水盾草与共生的本地沉水植物对本地牧食者的响应。我们进行了一个中宇宙尺度实验,研究了水盾草和两种共生的本地沉水植物黑藻(Hydrilla verticillata)和穗花狐尾藻(Myriophyllum spicatum)对两种本地广食性腹足纲螺类萝卜螺(Radix swinhoei)和环棱螺(Sinotaia quadrata)的牧食响应。记录了它们的形态性状指标(总生物量、冠根比和相对生长率)和生理性状指标(叶片总非结构性碳、木质素和纤维素)。研究结果表明,环棱螺对3种沉水植物性状指标的影响较少。随着本地广食性螺类萝卜螺数量的增加,黑藻和穗花狐尾藻大部分植物性状发生了改变,而水盾草的植物性状表现出相对稳定的趋势。水盾草对萝卜螺的牧食更具抵抗力,这与天敌逃逸假说的假设一致。这一发现说明牧食性螺类促进了水盾草的入侵,这可能会改变沉水植物群落中的物种组成。     

Protective Effect of Levilactobacillus brevis Against Yersinia enterocolitica Infection in Mouse Model via Regulating MAPK and NF-κB Pathway

Probiotics and Antimicrobial Proteins - Although the use of the probiotic bacterium Lactobacillus for the treatment and prevention of diseases caused by various pathogenic bacteria has received...     

七角星蜡蚧在云南省的分布与危害

[目的]七角星蜡蚧(半翅目:蚧次目:蚧科:蜡蚧属)是一种外来入侵害虫,自2013年在云南省西双版纳州勐仑镇发现以来,中国大陆再无系统性的调查。近年来作者在云南省收集蚧虫期间,在多地发现七角星蜡蚧为害,现对其在云南省的发生情况进行报道,以期为其监测和防治提供参考。[方法]2016年10月—2021年9月,对云南省15州(市)的64个县(区市)进行了系统调查,详细研究了七角星蜡蚧的分布、寄主及一些生物学习性。[结果]七角星蜡蚧在云南省西双版纳州的景洪市、勐腊县(勐仑镇和磨憨镇)、勐海县(打洛镇),普洱市的宁洱县,德宏州的盈江县(那邦镇),红河州的河口县、金平县(勐拉乡和者米乡)4州(市)7县(市)9地有分布,新增8个分布点。除宁洱县外,其余发生地均位于边境线上,表明其可能为境外多点入侵。在云南该虫危害寄主植物共4科4属5种,包括杧果和莲雾2种经济树种。七角星蜡蚧多寄生在叶片背面,沿叶脉分布,1年发生多代。在河口县的龙船花上,该虫每雌产卵38~50粒,虫口密度达185~250头·叶^-1。[结论]七角星蜡蚧在云南省零星分布,尚未造成严重损害,但有向内扩散的趋势,应加以警惕。     

Genome-scale analysis of Mannheimia succiniciproducens metabolism

Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is a capnophilic gram-negative bacterium that efficiently produces succinic acid, an industrially important four carbon dicarboxylic acid. In order to design a metabolically engineered strain which is capable of producing succinic acid with high yield and productivity, it is essential to optimize the whole metabolism at the systems level. Consequently, in silico modeling and simulation of the genome-scale metabolic network was employed for genome-scale analysis and efficient design of metabolic engineering experiments. The genome-scale metabolic network of M. succiniciproducens consisting of 686 reactions and 519 metabolites was constructed based on reannotation and validation experiments. With the reconstructed model, the network structure and key metabolic characteristics allowing highly efficient production of succinic acid were deciphered; these include strong PEP carboxylation, branched TCA cycle, relative weak pyruvate formation, the lack of glyoxylate shunt, and non-PTS for glucose uptake. Constraints-based flux analyses were then carried out under various environmental and genetic conditions to validate the genome-scale metabolic model and to decipher the altered metabolic characteristics. Predictions based on constraints-based flux analysis were mostly in excellent agreement with the experimental data. In silico knockout studies allowed prediction of new metabolic engineering strategies for the enhanced production of succinic acid. This genome-scale in silico model can serve as a platform for the systematic prediction of physiological responses of M. succiniciproducens to various environmental and genetic perturbations and consequently for designing rational strategies for strain improvement.     

Induction and preliminary characterization of a novel halophage SNJ1 from lysogenic Natrinema sp. F5

Halophage SNJ1 was induced with mitomycin C from Natrinema sp. strain F5. The phage produces plaques on Natrinema sp. strain J7 only. The phage has a head of about 67 nm in diameter and a tail of 570 nm in length and belongs morphologically to the family Siphoviridae. The phage is strongly salt dependent; NaCl concentration affects the integrity of SNJ1, phage adsorption, and plaque formation. The optimal NaCl concentration for phage adsorption and plaque formation is 30% and 25%, respectively.     

Surfactin from Bacillus subtilis displays anti-proliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression

The effect of surfactin on the proliferation of LoVo cells, a human colon carcinoma cell line, was examined. Surfactin strongly blocked the proliferation of LoVo cells by inducing pro-apoptotic activity and arresting the cell cycle, according to several lines of evidence on DNA fragmentation, Annexin V staining, and altered levels of poly (ADP-ribose) polymerase, caspase-3, p21(WAF1/Cip1), p53, CDK2 and cyclin E. The anti-proliferative activity of surfactin was mediated by inhibiting extracellular-related protein kinase and phosphoinositide 3-kinase/Akt activation, as assessed by phosphorylation levels. Therefore, our data suggest that surfactin may have anti-cancer properties as a result of its ability to downregulate the cell cycle and suppress its survival.