全文获取类型
收费全文 | 6815篇 |
免费 | 620篇 |
国内免费 | 2篇 |
出版年
2023年 | 39篇 |
2022年 | 42篇 |
2021年 | 109篇 |
2020年 | 65篇 |
2019年 | 59篇 |
2018年 | 113篇 |
2017年 | 124篇 |
2016年 | 170篇 |
2015年 | 243篇 |
2014年 | 275篇 |
2013年 | 366篇 |
2012年 | 452篇 |
2011年 | 426篇 |
2010年 | 288篇 |
2009年 | 264篇 |
2008年 | 383篇 |
2007年 | 376篇 |
2006年 | 337篇 |
2005年 | 328篇 |
2004年 | 343篇 |
2003年 | 313篇 |
2002年 | 304篇 |
2001年 | 185篇 |
2000年 | 172篇 |
1999年 | 136篇 |
1998年 | 92篇 |
1997年 | 47篇 |
1996年 | 76篇 |
1995年 | 51篇 |
1994年 | 54篇 |
1993年 | 47篇 |
1992年 | 86篇 |
1991年 | 79篇 |
1990年 | 81篇 |
1989年 | 64篇 |
1988年 | 58篇 |
1987年 | 47篇 |
1986年 | 52篇 |
1985年 | 49篇 |
1984年 | 55篇 |
1983年 | 48篇 |
1982年 | 35篇 |
1981年 | 29篇 |
1980年 | 39篇 |
1979年 | 39篇 |
1978年 | 46篇 |
1977年 | 38篇 |
1976年 | 52篇 |
1975年 | 29篇 |
1973年 | 28篇 |
排序方式: 共有7437条查询结果,搜索用时 15 毫秒
101.
Reproductive traits that function in pollinator attraction may be reduced or lost during evolutionary transitions from outcrossing to selfing. Although floral scent plays an important role in attracting pollinators in outcrossing species, few studies have investigated associations between floral scent variation and intraspecific mating system transitions. The breakdown of distyly to homostyly represents a classic example of a shift from outcrossing to selfing and provides an opportunity to test whether floral fragrances have become reduced and/or changed in composition with increased selfing. Here, we evaluate this hypothesis by quantifying floral volatiles using gas chromatography-mass spectrometry in two distylous and four homostylous populations of Primula oreodoxa Franchet, a perennial herb from SW China. Our analysis revealed significant variation of volatile organic compounds (VOCs) among populations of P. oreodoxa. Although there was no difference in VOCs between floral morphs in distylous populations as predicted, we detected a substantial reduction in VOC emissions and the average number of scent compounds in homostylous compared with distylous populations. A total of 12 compounds, mainly monoterpenoids and sesquiterpenoids, distinguished homostylous and distylous morphs; of these, (E)-β-ocimene was the most important in contributing to the difference in volatiles, with significantly lower emissions in homostyles. Our findings support the hypothesis that the transition from outcrossing to selfing is accompanied by the loss of floral volatiles. The modification to floral fragrances in P. oreodoxa associated with mating system change might occur because high selfing rates in homostylous populations result in relaxed selection for floral attractiveness. 相似文献
102.
J Jiménez-Castellanos A Carmona C J Catalina J Jiménez-Castellanos 《Acta anatomica》1992,143(3):182-187
In the present paper, we describe anatomical variants of the anterior inferior cerebellar artery in man for applicative purposes. Our goal was to provide the surgeon with a detailed anatomical view of the region. This is similar to what he may observe through the surgical microscope using modern microsurgical techniques. We have focused our attention on the segments of the artery comprising its origin, its course until it reaches the cerebellum and its main collateral branches. Our results confirm the great variability of the elements under study, but enable the establishment of a few basic variational patterns. These patterns together with their relative frequency may be helpful in microsurgery. 相似文献
103.
104.
Production of 6-Phenylacetylene Picolinic Acid from Diphenylacetylene by a Toluene-Degrading Acinetobacter Strain
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jim C. Spain Shirley F. Nishino Bernard Witholt Loon-Seng Tan Wouter A. Duetz 《Applied microbiology》2003,69(7):4037-4042
Several strategies for using enzymes to catalyze reactions leading to the synthesis of relatively simple substituted picolinic acids have been described. The goal of the work described here was to synthesize a more complex molecule, 6-phenylacetylene picolinic acid [6-(2-phenylethynyl)pyridine-2-carboxylic acid], for use as a potential endcapping agent for aerospace polymers. We screened 139 toluene-degrading strains that use a variety of catabolic pathways for the ability to catalyze oxidative transformation of diphenylacetylene. Acinetobacter sp. strain F4 catalyzed the overall conversion of diphenylacetylene to a yellow metabolite, which was identified as a putative meta ring fission product (2-hydroxy-8-phenyl-6-oxoocta-2,4-dien-7-ynoic acid [RFP]). The activity could be sustained by addition of toluene at a flow rate determined empirically so that the transformations were sustained in spite of the fact that toluene is a competitive inhibitor of the enzymes. The overall rate of transformation was limited by the instability of RFP. The RFP was chemically converted to 6-phenylacetylene picolinic acid by treatment with ammonium hydroxide. The results show the potential for using the normal growth substrate to provide energy and to maintain induction of the enzymes involved in biotransformation during preliminary stages of biocatalyst development. 相似文献
105.
106.
107.
G. Wayne Brodland Michael J. Scott Andrew F. MacLean M. Globus S. Vethamany-Globus R. Gordon Jim H. Veldhuis R. Del Maestro 《Development genes and evolution》1996,205(5-6):311-318
During neurulation in vertebrate embryos, epithelial cells of the neural plate undergo complex morphogenetic movements that culminate in rolling of the plate into a tube. Resolution of the determinants of this process requires an understanding of the precise movements of cells within the epithelial sheet. A computer algorithm that allows automated tracking of epithelial cells visible in digitized video images is presented. It is used to quantify the displacement field associated with morphogenetic movements in the axolotl (Ambystoma mexicanum) neural plate during normal neural tube formation. Movements from lateral to medial, axial elongations and area changes are calculated from the displacement field data and plotted as functions of time. Regional and temporal differences are identified. The approach presented is suitable for analyzing a wide variety of morphogenetic movements. 相似文献
108.
David E. Holm Gerald Godette Celia Bonaventura Joseph Bonaventura M. David Boatright Linda L. Pearce Jim Peterson 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1996,114(4):345-352
Contrary to previous reports, the functional and spectral properties of “monomeric” shark cytochrome c oxidases are not entirely similar to those of the “dimeric” beef enzyme. Most significantly, unlike the behavior of beef oxidase, the fully oxidized shark enzyme is not reducible by carbon monoxide. Also, preparations of the shark enzyme, isolated at pH 7.8-8.0, lead to more than 60% of the sample always being obtained in a resting form, whereas similarly prepared beef oxidase is very often obtained, both by ourselves and others, exclusively in the pulsed form. Although the electronic absorption, magnetic circular dichroism and electron paramagnetic resonance (EPR) spectra of cytochrome c oxidase obtained from several shark species are similar to those of the beef enzyme, there are some significant differences. In particular, the Soret maximum is at 422 nm in the case of the fully oxidized resting shark oxidases at physiological pH and not 418 nm as commonly found for the beef enzyme. Moreover, the resting shark oxidases do not necessarily exhibit a “g = 12” signal in their EPR spectra. The turnover numbers of recent preparations of the shark enzyme are higher than previously reported and, interestingly, do not differ within experimental uncertainty from those documented for several beef isoenzymes assayed under comparable conditions. 相似文献
109.
J. Carl Barrett 《Mutation research》1995,333(1-2):189-202
Species differences resulting from a number of mechanisms are common in receptor-mediated chemical carcinogenesis. In this review, examples of possible mechanisms underlying these differences are discussed, including ligand metabolism, receptor polymorphisms, receptor isoforms, receptor levels, and crosstalk between signal transduction pathways. In addition, a number of other mechanisms also are likely to be important. The developmental state of the animal will determine the expression of receptors in different tissues. The regulatory pathways for cell proliferation and cell death and cell cycle check point controls can vary among species and tissues. Adaptation or potentiation of responses during chronic exposures to chemicals can greatly influence species differences. The mechanisms of adaptive processes are poorly understood but probably highly important for chronic toxicities such as cancer. Finally, different species may have different stem cell populations that are the targets for neoplastic transformation, and this will influence receptor-mediated carcinogenic responses. The implications of species differences in receptor-mediated responses for risk assessment are discussed. 相似文献
110.