全文获取类型
收费全文 | 6815篇 |
免费 | 620篇 |
国内免费 | 2篇 |
出版年
2023年 | 39篇 |
2022年 | 42篇 |
2021年 | 109篇 |
2020年 | 65篇 |
2019年 | 59篇 |
2018年 | 113篇 |
2017年 | 124篇 |
2016年 | 170篇 |
2015年 | 243篇 |
2014年 | 275篇 |
2013年 | 366篇 |
2012年 | 452篇 |
2011年 | 426篇 |
2010年 | 288篇 |
2009年 | 264篇 |
2008年 | 383篇 |
2007年 | 376篇 |
2006年 | 337篇 |
2005年 | 328篇 |
2004年 | 343篇 |
2003年 | 313篇 |
2002年 | 304篇 |
2001年 | 185篇 |
2000年 | 172篇 |
1999年 | 136篇 |
1998年 | 92篇 |
1997年 | 47篇 |
1996年 | 76篇 |
1995年 | 51篇 |
1994年 | 54篇 |
1993年 | 47篇 |
1992年 | 86篇 |
1991年 | 79篇 |
1990年 | 81篇 |
1989年 | 64篇 |
1988年 | 58篇 |
1987年 | 47篇 |
1986年 | 52篇 |
1985年 | 49篇 |
1984年 | 55篇 |
1983年 | 48篇 |
1982年 | 35篇 |
1981年 | 29篇 |
1980年 | 39篇 |
1979年 | 39篇 |
1978年 | 46篇 |
1977年 | 38篇 |
1976年 | 52篇 |
1975年 | 29篇 |
1973年 | 28篇 |
排序方式: 共有7437条查询结果,搜索用时 15 毫秒
11.
Jim Orford 《BMJ (Clinical research ed.)》1988,296(6624):729-730
12.
13.
14.
15.
16.
Phosphorylation, glycosylation, and proteolytic activity of the 52-kD estrogen-induced protein secreted by MCF7 cells 总被引:4,自引:1,他引:3
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
F Capony M Morisset A J Barrett J P Capony P Broquet F Vignon M Chambon P Louisot H Rochefort 《The Journal of cell biology》1987,104(2):253-262
We have studied the posttranslational modifications of the 52-kD protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (Westley, B., and H. Rochefort, 1980, Cell, 20:353-362). The secreted 52-kD protein was found to be phosphorylated mostly (94%) on high-mannose N-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. The phosphate signal was totally removed by alkaline phosphatase hydrolysis. The secreted 52-kD protein was partly taken up by MCF7 cells via mannose-6-phosphate receptors and processed into 48- and 34-kD protein moieties as with lysosomal hydrolases. By electron microscopy, immunoperoxidase staining revealed most of the reactive proteins in lysosomes. After complete purification by immunoaffinity chromatography, we identified both the secreted 52-kD protein and its processed cellular forms as aspartic and acidic proteinases specifically inhibited by pepstatin. The 52-kD protease is secreted in breast cancer cells under its inactive proenzyme form, which can be autoactivated at acidic pH with a slight decrease of molecular mass. The enzyme of breast cancer cells, when compared with cathepsin D(s) of normal tissue, was found to be similar in molecular weight, enzymatic activities (inhibitors, substrates, specific activities), and immunoreactivity. However, the 52-kD protein and its cellular processed forms of breast cancer cells were totally sensitive to endo-beta-N-acetylglucosaminidase H (Endo H), whereas several cellular cathepsin D(s) of normal tissue were partially Endo H-resistant. This difference, in addition to others concerning tissue distribution, mitogenic activity and hormonal regulation, strongly suggests that the 52-kD cathepsin D-like enzyme of breast cancer cells is different from previously described cathepsin D(s). The 52-kD estrogen-induced lysosomal proteinase may have important functions in facilitating the mammary cancer cells to proliferate, migrate, and metastasize. 相似文献
17.
T J Kinsella P P Dobson A J Fornace S F Barrett M B Ganges J H Robbins 《Biochemical and biophysical research communications》1987,149(2):355-361
Cultured fibroblast strains from two normal persons and from two patients with the neurodegeneration of Alzheimer's disease were exposed to the alkylating chemical N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Immediately after exposure and also after a 24-h repair incubation period the single-strand breaks in the cells' DNA were quantified by the alkaline elution technique. In contrast to a report by others using alkaline elution, MNNG, and these same strains, we found no evidence of deficient repair of MNNG-induced DNA damage in the Alzheimer's disease cells. The putative DNA repair defect in Alzheimer's disease should be investigated by methods other than the alkaline elution technique which measures only a small fraction of the damage induced by an alkylating chemical such as MNNG. 相似文献
18.
A sensitive gel retention assay has been utilized to detect proteins from uninfected Hela nuclei which interact with the adenovirus type 2 enhancer. This assay has been employed to monitor fractionation of nuclear extracts. Three enhancer binding factors were resolved by chromatography on DEAE-Sepharose and one of the factors was further purified by chromatography on heparin-Sepharose. DNase protection experiments have shown that the heparin-Sepharose fraction contains a factor which binds predominantly to the conserved sequence GTGGAAATTT present at position 160 in the adenovirus type 2 genome and found in many viral and cellular enhancers. Protection of this sequence from DNase I digestion was abolished by competition with a synthetic duplex oligonucleotide spanning bases 144-181. This region corresponds to the sequence defined by Hen et al. as possessing enhancer function. Competition experiments indicated that the enhancer binding factor also bound, albeit with reduced affinity, to multiple sites in the Ela upstream region located between positions 192 and 353. Within the sequences which compete are regions with homology to the high affinity site at position 160. The enhancer binding factor also binds with high affinity to sequences within the SV40 enhancer demonstrating that this factor interacts with sequences common to both the adenovirus and SV40 enhancers. 相似文献
19.
20.
Skeletal muscle growth, muscle nucleic acids and muscle protein synthesis capacity, were measured to evaluate the protein requirement of adult rats. Wistar rats were fed on diets containing 4%, 10% or 20% casein + D,L-methionine. All diets were provided for 21 days beginning at 90 days of age. Body weight, food efficiency and net weight change increased as the casein content of the diet increased. Muscle DNA, RNA and RNA/protein were lost, but protein and protein/DNA increased on the 4% and 20% protein diet. This fact involves an aplasia phenomenon although the hypertrophic growth is maintained. Alterations of the insulin and GH plasma levels were observed. These findings indicate that for adult rats the 4% and 20% protein diets are not adequate for the period of adult maintenance. 相似文献