全文获取类型
收费全文 | 6865篇 |
免费 | 618篇 |
国内免费 | 12篇 |
专业分类
7495篇 |
出版年
2024年 | 7篇 |
2023年 | 70篇 |
2022年 | 142篇 |
2021年 | 243篇 |
2020年 | 104篇 |
2019年 | 172篇 |
2018年 | 191篇 |
2017年 | 190篇 |
2016年 | 247篇 |
2015年 | 367篇 |
2014年 | 372篇 |
2013年 | 469篇 |
2012年 | 566篇 |
2011年 | 504篇 |
2010年 | 321篇 |
2009年 | 252篇 |
2008年 | 353篇 |
2007年 | 357篇 |
2006年 | 312篇 |
2005年 | 302篇 |
2004年 | 227篇 |
2003年 | 207篇 |
2002年 | 232篇 |
2001年 | 150篇 |
2000年 | 192篇 |
1999年 | 133篇 |
1998年 | 53篇 |
1997年 | 31篇 |
1996年 | 36篇 |
1995年 | 47篇 |
1994年 | 35篇 |
1993年 | 35篇 |
1992年 | 70篇 |
1991年 | 59篇 |
1990年 | 58篇 |
1989年 | 60篇 |
1988年 | 45篇 |
1987年 | 33篇 |
1986年 | 43篇 |
1985年 | 44篇 |
1984年 | 26篇 |
1983年 | 22篇 |
1982年 | 15篇 |
1981年 | 16篇 |
1980年 | 8篇 |
1979年 | 15篇 |
1978年 | 12篇 |
1977年 | 12篇 |
1976年 | 7篇 |
1973年 | 7篇 |
排序方式: 共有7495条查询结果,搜索用时 15 毫秒
151.
Bo-Hwa Choi Souvik Chattopadhaya Le Nguyen Thanh Lin Feng Quoc Toan Nguyen Chuan Bian Lim Amaravadhi Harikishore Ravi Prakash Reddy Nanga Nagakumar Bharatham Yan Zhao Xuewei Liu Ho Sup Yoon 《PloS one》2014,9(10)
Microtubules are a highly validated target in cancer therapy. However, the clinical development of tubulin binding agents (TBA) has been hampered by toxicity and chemoresistance issues and has necessitated the search for new TBAs. Here, we report the identification of a novel cell permeable, tubulin-destabilizing molecule - 4,5,6,7-tetrahydro-1H-indazole-3-carboxylic acid [1p-tolyl-meth-(E)-ylidene]-hydrazide (termed as Suprafenacine, SRF). SRF, identified by in silico screening of annotated chemical libraries, was shown to bind microtubules at the colchicine-binding site and inhibit polymerization. This led to G2/M cell cycle arrest and cell death via a mitochondria-mediated apoptotic pathway. Cell death was preceded by loss of mitochondrial membrane potential, JNK - mediated phosphorylation of Bcl-2 and Bad, and activation of caspase-3. Intriguingly, SRF was found to selectively inhibit cancer cell proliferation and was effective against drug-resistant cancer cells by virtue of its ability to bypass the multidrug resistance transporter P-glycoprotein. Taken together, our results suggest that SRF has potential as a chemotherapeutic agent for cancer treatment and provides an alternate scaffold for the development of improved anti-cancer agents. 相似文献
152.
Ranad Shaheen Hanan E. Shamseldin Catrina M. Loucks Mohammed Zain Seidahmed Shinu Ansari Mohamed Ibrahim Khalil Nadya Al-Yacoub Erica E. Davis Natalie A. Mola Katarzyna Szymanska Warren Herridge Albert E. Chudley Bernard N. Chodirker Jeremy Schwartzentruber Jacek Majewski Nicholas Katsanis Coralie Poizat Colin A. Johnson Jillian Parboosingh Kym M. Boycott A. Micheil Innes Fowzan S. Alkuraya 《American journal of human genetics》2014
153.
A. R. Kleinig C. J. Mansell Q. D. Nguyen A. Badalyan A. P. J. Middelberg 《Biotechnology Techniques》1995,9(10):759-762
Summary The effect of cell concentration (5 to 150 g/L wet wt after broth dilution) on homogenizer disruption efficiency and homogenate viscosity is reported for E. coli. Broth dilution increases homogenizer efficiency and decreases feed and homogenate viscosity. However, this increase in disruption efficiency is not sufficient to warrant dilution of the broth prior to homogenization. The optimal feed concentration is the maximum possible that does not lead to practical handling difficulties due to high viscosity. 相似文献
154.
A nematode collected from Diaprepes abbreviatus is identified and described as a new species, Steinernema diaprepesi n. sp. The new species is closely related to S. feltiae, S. glaseri, and S. oregonense and can be distinguished from these species by the following characteristics: Males: Spicule averaging 79 (71-90) µm and spicule shape; D% (distance from anterior end to excretory pore/ esophagus length × 100) about 80; the ratio SW (spicule length/anal body width) about 1.8. Females: Vulva with short, double- flapped epiptygma; tail terminus usually with 5 papillae-like structures. Infective juveniles: Body averaging 1,002 (880-1,133) µm, EP (distance from anterior end to excretory pore) = 74 (66-83) µm; tail length = 83 (65-91) µm, and E% (EP/tail length × 100) = 89.6 (78-114). Lateral field pattern variable, the formula for the arrangement of ridges from head to tail is: 2, 6, 7, 8, 4, 2. The portion with eight ridges is the longest. This new species can be differentiated further from three closest species (S. feltiae, S. glaseri, and S. oregonense) by characteristic sequences of their ITS regions, including sequence lengths, ratios of similarity, composition, and differences in base characters in sequence alignment. 相似文献
155.
Nguyen Thuy Trang Nguyen Thi Thuy Dung Tran Nguyen-Minh-An Van Vo Giau 《Neurochemical research》2022,47(3):552-573
Neurochemical Research - Neurodegenerative disorders are distinguished by the gradual deterioration of the nervous system’s structure and function due to oxidative stress, mitochondrial... 相似文献
156.
Jelena Petrovic Yeqiao Zhou Maria Fasolino Naomi Goldman Gregory W. Schwartz Maxwell R. Mumbach Son C. Nguyen Kelly S. Rome Yogev Sela Zachary Zapataro Stephen C. Blacklow Michael J. Kruhlak Junwei Shi Jon C. Aster Eric F. Joyce Shawn C. Little Golnaz Vahedi Warren S. Pear Robert B. Faryabi 《Molecular cell》2019,73(6):1174-1190.e12
157.
158.
Wang W Nguyen NM Agapov E Holtzman MJ Woods JC 《Journal of applied physiology (Bethesda, Md. : 1985)》2012,112(9):1593-1599
Recently, a Sendai virus (SeV) model of chronic obstructive lung disease has demonstrated an innate immune response in mouse airways that exhibits similarities to the chronic airway inflammation in human chronic obstructive pulmonary disease (COPD) and asthma, but the effect on distal lung parenchyma has not been investigated. The aim of our study is to image the time course and regional distribution of mouse lung microstructural changes in vivo after SeV infection. (1)H and (3)He diffusion magnetic resonance imaging (MRI) were successfully performed on five groups of C57BL/6J mice. (1)H MR images provided precise anatomical localization and lung volume measurements. (3)He lung morphometry was implemented to image and quantify mouse lung geometric microstructural parameters at different time points after SeV infection. (1)H MR images detected the SeV-induced pulmonary inflammation in vivo; spatially resolved maps of acinar airway radius R, alveolar depth h, and mean linear intercept Lm were generated from (3)He diffusion images. The morphometric parameters R and Lm in the infected group were indistinguishable from PBS-treated mice at day 21, increased slightly at day 49, and were increased with statistical significance at day 77 (p = 0.02). Increases in R and Lm of infected mice imply that there is a modest increase in alveolar duct radius distal to airway inflammation, particularly in the lung periphery, indicating airspace enlargement after virus infection. Our results indicate that (3)He lung morphometry has good sensitivity in quantifying small microstructural changes in the mouse lung and that the Sendai mouse model has the potential to be a valid murine model of COPD. 相似文献
159.
Masashi Kato Mayuko Y. Kumasaka Shoko Ohnuma Akio Furuta Yoko Kato Hossain U. Shekhar Michiyo Kojima Yasuko Koike Nguyen Dinh Thang Nobutaka Ohgami Thuy Bich Ly Xiaofang Jia Husna Yetti Hisao Naito Gaku Ichihara Ichiro Yajima 《PloS one》2013,8(6)
Health risk for well drinking water is a worldwide problem. Our recent studies showed increased toxicity by exposure to barium alone (≤700 µg/L) and coexposure to barium (137 µg/L) and arsenic (225 µg/L). The present edition of WHO health-based guidelines for drinking water revised in 2011 has maintained the values of arsenic (10 µg/L) and barium (700 µg/L), but not elements such as manganese, iron and zinc. Nevertheless, there have been very few studies on barium in drinking water and human samples. This study showed significant correlations between levels of arsenic and barium, but not its homologous elements (magnesium, calcium and strontium), in urine, toenail and hair samples obtained from residents of Jessore, Bangladesh. Significant correlation between levels of arsenic and barium in well drinking water and levels in human urine, toenail and hair samples were also observed. Based on these results, a high-performance and low-cost adsorbent composed of a hydrotalcite-like compound for barium and arsenic was developed. The adsorbent reduced levels of barium and arsenic from well water in Bangladesh and Vietnam to <7 µg/L within 1 min. Thus, we have showed levels of arsenic and barium in humans and propose a novel remediation system. 相似文献
160.
Evrard JL Nguyen I Bergdoll M Mutterer J Steinmetz A Lambert AM 《Plant molecular biology》2002,49(6):611-620
We describe here a new -tubulin isoform from sunflower we named -tubulin. -tubulin is the most divergent higher-plant -tubulin described so far, having an unusual deletion in the H1/B2 loop and a glutamine-rich C-terminus. We constructed a three-dimensional model and discuss its implications. Using specific antibodies, we show that -tubulin expression is restricted to the male gametophyte. -tubulin mRNA represents 90% of -tubulin mRNA and a small percentage of total pollen mRNA. Among the plants tested, -tubulin was only detected in sunflower and in Cosmos. Since both plants are Asteraceae, we propose that -tubulin is specific to this family. Our results suggest that -tubulin can inhibit tubulin assembly in pollen. This hypothesis is reinforced by the fact that -tubulin is found in a complex with -tubulin in mature sunflower pollen. 相似文献