全文获取类型
收费全文 | 4972篇 |
免费 | 448篇 |
国内免费 | 5篇 |
出版年
2022年 | 24篇 |
2021年 | 78篇 |
2020年 | 57篇 |
2019年 | 67篇 |
2018年 | 89篇 |
2017年 | 71篇 |
2016年 | 114篇 |
2015年 | 237篇 |
2014年 | 246篇 |
2013年 | 297篇 |
2012年 | 384篇 |
2011年 | 351篇 |
2010年 | 235篇 |
2009年 | 197篇 |
2008年 | 264篇 |
2007年 | 290篇 |
2006年 | 286篇 |
2005年 | 249篇 |
2004年 | 294篇 |
2003年 | 239篇 |
2002年 | 178篇 |
2001年 | 96篇 |
2000年 | 73篇 |
1999年 | 98篇 |
1998年 | 78篇 |
1997年 | 50篇 |
1996年 | 39篇 |
1995年 | 28篇 |
1994年 | 48篇 |
1993年 | 39篇 |
1992年 | 45篇 |
1991年 | 53篇 |
1990年 | 40篇 |
1989年 | 32篇 |
1988年 | 31篇 |
1987年 | 34篇 |
1986年 | 38篇 |
1985年 | 39篇 |
1984年 | 22篇 |
1983年 | 25篇 |
1982年 | 30篇 |
1981年 | 23篇 |
1980年 | 21篇 |
1979年 | 23篇 |
1978年 | 27篇 |
1976年 | 21篇 |
1975年 | 18篇 |
1974年 | 18篇 |
1973年 | 18篇 |
1972年 | 15篇 |
排序方式: 共有5425条查询结果,搜索用时 0 毫秒
21.
Water and polypeptide conformations in the gramicidin channel. A molecular dynamics study. 总被引:15,自引:14,他引:1
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Theoretical studies of ion channels address several important questions. The mechanism of ion transport, the role of water structure, the fluctuations of the protein channel itself, and the influence of structural changes are accessible from these studies. In this paper, we have carried out a 70-ps molecular dynamics simulation on a model structure of gramicidin A with channel waters. The backbone of the protein has been analyzed with respect to the orientation of the carbonyl and the amide groups. The results are in conformity with the experimental NMR data. The structure of water and the hydrogen bonding network are also investigated. It is found that the water molecules inside the channel act as a collective chain; whereas the conformation in which all the waters are oriented with the dipoles pointing along the axis of the channel is a preferred one, others are also accessed during the dynamics simulation. A collective coordinate involving the channel waters and some of the hydrogen bonding peptide partners is required to describe the transition of waters from one configuration to the other. 相似文献
22.
Human metallothionein genes: structure of the functional locus at 16q13 总被引:21,自引:0,他引:21
The functional human metallothionein (MT) genes are located on chromosome 16q13. We have physically mapped the functional human MT locus by isolation and restriction digest mapping of cloned DNA. The mapped region contains all sequences on chromosome 16 that hybridize to metallothionein gene probes and comprises 14 tightly linked MT genes, 6 of which have not been previously described. This analysis defines the genetic limits of metallothionein functional diversity in the human genome. 相似文献
23.
24.
25.
Three-dimensional structure of rotavirus 总被引:40,自引:0,他引:40
26.
27.
28.
29.
Alignment and merging of electron microscope images of frozen hydrated crystals of the T4 DNA helix destabilizing protein gp32*I. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Low dose cryoelectron microscopy has been used to record images and electron diffraction patterns of frozen hydrated crystals of the single-stranded DNA binding protein gp32*I. Fourier transforms from 13 image areas, corresponding to approximately 40,000 unit cells, were aligned by a minimal phase residual search and merged by vector addition in reciprocal space. Phases from the resulting composite transform were combined with amplitudes from electron diffraction patterns to reconstruct the projected mass density of the gp32*I crystal at 8.4 A resolution. 相似文献
30.