吉林省松花湖库区台蚱属一新种(直翅目,蚱总科)

记述采自吉林省吉林市松花湖库区的蚱总科台蚱属1新种：长角台蚱Formosatettix longicornia sp.nov．。     

二爪螨属和中(虫穴)螨属各一新种(蜱螨亚纲,前爪螨科,(虫穴)螨科)

记述尾足螨股前爪螨科二爪螨属1新种,具齿二爪螨Dinychus dentatus sp.nov.和革螨股(虫穴)螨科中(虫穴)螨属1新种,敦化中(虫穴)螨Mesozercon dunhuaensis sp.nov.,中(虫穴)螨属在中国为首次记录.     

枝厉螨属(胭螨科)和黑面螨属(黑面螨科)各一新种(蜱螨亚纲,中气门亚目)

记述革螨股Gamasina1新种,神农架枝厉螨Dendrolaelaps shennongjiaensis Ma et Liu,sp.nov,和角螨股Antennophorina1新种;新疆黑面螨Celaenopsis xinjiangensis Ma et Yc,sp.nov,角螨股在中国为首次记录。     

吉林省担子菌补记(五)

报道了 6种吉林省新记录担子菌 ,即斑玉蕈 [Hypsizygusmarmoreus (Peck)Bigelow],大幕侧耳[Pleurotuscalyptratus (Lindbl :Fr )Sacc ],矮包脚菇 [Volvariellapusilla (Pers :Fr )Sing ],白顶丽蘑[Calocybeleucocephala (Fr )Qu啨ｌ ],彩丽小菇 (MycenapictaHarmaja)和平截棒瑚菌 [Clavariadelphustruncatus(Qu啨ｌ )Donk ]。其中 ,斑玉蕈为首次在我国发现。     

Effects of Methanol on Wettability of the Non-Smooth Surface on Butterfly Wing

The contact angles of distilled water and methanol solution on the wings of butterflies were determined by a visual contact angle measuring system. The scale structures of the wings were observed using scanning electron microscopy, The influence of the scale micro- and ultra-structure on the wettability was investigated. Results show that the contact angle of distilled water on the wing surfaces varies from 134.0° to 159.2°. High hydrophobicity is found in six species with contact angles greater than 150°. The wing surfaces of some species are not only hydrophobic but also resist the wetting by methanol solution with 55% concentration. Only two species in Parnassius can not resist the wetting because the micro-structure （spindle-like shape） and ultra-structure （pinnule-like shape） of the wing scales are remarkably different from that of other species. The concentration of methanol solution for the occurrence of spreading/wetting on the wing surfaces of different species varies from 70% to 95%. After wetting by methanol solution for 10 min, the distilled water contact angle on the wing surface increases by 0.8°-2.1°, showing the promotion of capacity against wetting by distilled water.     

Expression, purification and characterization of recombinant mouse translation initiation factor eIF4E as a dihydrofolate reductase (DHFR) fusion protein

One of the earliest steps in translation initiation is recognition of the mRNA cap structure (m7GpppX) by the initiation factor eIF4E. Studies of interactions between purified eIF4E and its binding partners provide important information for understanding mechanisms underlying translational control in normal and cancer cells. Numerous impediments of the available methods used for eIF4E purification led us to develop a novel methodology for obtaining fractions of eIF4E free from undesired by-products. Herein we report methods for bacterial expression of eIF4E tagged with mutant dihydrofolate reductase (DHFR) followed by isolation and purification of the DHFR–eIF4E protein by using affinity and anion exchange chromatography. Fluorescence quenching experiments indicated the cap-analog, 7MeGTP, bound to DHFR–eIF4E and eIF4E with a dissociation constant (K_d) of 6 ± 5 and 10 ± 3 nM, respectively. Recombinant eIF4E and DHFR–eIF4E were both shown to significantly enhance in vitro translation in dose dependent manner by 75% at 0.5 μM. Nevertheless increased concentrations of eIF4E and DHFR–eIF4E significantly inhibited translation in a dose dependent manner by a maximum at 2 μM of 60% and 90%, respectively. Thus, we have demonstrated that we have developed an expression system for fully functional recombinant eIF4E. We have also shown that the fusion protein DHFR–eIF4E is functional and thus may be useful for cell based affinity tag studies with fluorescently labeled trimethoprim analogs.     

Gabor Filter Optimization Design for Iris Texture Analysis

1　IntroductionIrisrecognitiontechniqueisbasedonthestableanddistinctiveirispatterns,andhasattractedmuchat tentionofsomeexpertsforthelastfewdecades .Sincethe 1990’s ,muchworkonirisrecognitionhasbeendoneandgreatprogresshasbeenmade[1- 4] .Nowadaysithasbecomeamostimportantbiometricsolutionforpersonalidentification .Anirishasanapproximatelycircularannularshape.Theiristexturehasarandomlydistributedstructureofdetails ,whichiscreatedbyradialandcon centricfurrows,pigmentspots,crypts ,pigmentfrillandc…     

Concentration-dependent behavior of nisin interaction with supported bilayer lipid membrane

Nisin is a positively charged antibacterial peptide that binds to the negatively charged membranes of gram-positive bacteria. The initial interaction of the peptide with the model membrane of negatively charged DPPG (dipalmitoylphosphatidylglycerol) was studied by cyclic voltammetry and a.c. impedance spectroscopy. Nisin could induce pores in the supported bilayer lipid membrane, thus, it led to the marker ions Fe(CN)(6)(3-/4-) crossing the lipid membrane and giving the redox reaction on the glassy carbon electrode (GCE). Experimental results suggested that the pore formation on supported bilayer lipid membrane was dependent on the concentration of nisin and it included three main concentration stages: low, middling, high concentration.     

A2E-epoxides damage DNA in retinal pigment epithelial cells. Vitamin E and other antioxidants inhibit A2E-epoxide formation

The autofluorescent pigments that accumulate in retinal pigment epithelial cells with aging and in some retinal disorders have been implicated in the etiology of macular degeneration. The major constituent is the fluorophore A2E, a pyridinium bisretinoid. Light-exposed A2E-laden retinal pigment epithelium exhibits a propensity for apoptosis with light in the blue region of the spectrum being most damaging. Efforts to understand the events precipitating the death of the cells have revealed that during irradiation (430 nm), A2E self-generates singlet oxygen with the singlet oxygen in turn reacting with A2E to generate epoxides at carbon-carbon double bonds. Here we demonstrate that A2E-epoxides, independent of singlet oxygen, exhibit reactivity toward DNA with oxidative base changes being at least one of these lesions. Mass spectrometry revealed that the antioxidants vitamins E and C, butylated hydroxytoluene, resveratrol, a trolox analogue (PNU-83836-E), and bilberry extract reduce A2E-epoxidation, whereas single cell gel electrophoresis and cell viability studies revealed a corresponding reduction in the incidence of DNA damage and cell death. Vitamin E, a lipophilic antioxidant, produced a more pronounced decrease in A2E-epoxidation than vitamin C, and treatment with both vitamins simultaneously did not confer additional benefit. Studies in which singlet oxygen was generated by endoperoxide in the presence of A2E revealed that vitamin E, butylated hydroxytoluene, resveratrol, the trolox analogue, and bilberry reduced A2E-epoxidation by quenching singlet oxygen. Conversely, vitamin C and ginkgolide B were not efficient quenchers of singlet oxygen under these conditions.     

利用RNA干扰技术获得晚抽薹开花转基因大白菜

LEAFY基因在植物的成花转变中起重要作用。在拟南芥中,降低LEAFY基因的表达使开花延迟,并且增加分枝。利用RNAi技术降低大白菜LEAFY基因表达,获得晚抽薹开花的转基因大白菜。开花时转基因大白菜的株高明显低于对照,而叶片数、叶面积和侧枝数则高于对照;转基因植株的LEAFY基因表达明显降低,导致大白菜抽薹开花延迟。因此可以通过降低LEAFY基因表达来延迟需低温春化作用长日植物开花。也为利用生物技术获得晚抽薹转基因大白菜提供了理论依据。