7个大穗型小麦-黑麦代换系间杂交后代的形态学与SSR分析

对从小麦-黑麦代换系5R/5A与1R/1D杂交后代中选育的大穗型品系1-5、1-6、1-7、1-8、1-9、1-10、1-11进行形态学与SSR分析。结果表明,7个品系田间表现遗传性稳定,且具有黑麦大穗、抗病等优良性状;利用黑麦特异引物PSC119.1确定7个品系均导入了黑麦染色体片段,引物SCM138扩增结果表明,在1-6、1-7、1-8、1-9、1-10中导入了黑麦染色体5RS片段,引物SCM120、TSM604可以在7个品系中稳定扩增出黑麦5R长臂和1R短臂特异片段。以上结果可为小麦遗传育种与品质改良提供基础材料。     

Response of Pyropia haitanensis to agaro-oligosaccharides evidenced mainly by the activation of the eicosanoid pathway

The marine red alga Pyropia haitanensis (Protoflorideophyceae, Bangiaceae) has a nonvascular and multicellular structure and emerged earlier in evolution than other cultivatable red algae. It has been reported that lipid mediators from both the eicosanoid and octadecanoid pathways are involved in the innate immunity of other marine algae. But the defense strategies of P. haitanensis are not clearly understood. Here, we investigated the lipid defense of P. haitanensis elicited by agaro-oligosaccharides. The results indicate that the resistance of P. haitanensis was elicited and hydrogen peroxide was released by agaro-oligosaccharides. In P. haitanensis, C20 fatty acids are the essential fatty acids. Phospholipase A₂ was activated, and the free fatty acids decreased 3 h after treatment with agaro-oligosaccharides. Gas chromatography–mass spectrometry analyses revealed that the contents of volatile organic compounds increased after treatment for 3 h, which indicated that these free fatty acids were metabolized to volatile organic compounds. In conclusion, the lipid metabolic defense pathway of P. haitanensis was mainly via the C20 metabolism pathway. The C20 fatty acid was rapidly metabolized to volatile organic compounds, but not oxidized to oxylipins in response to agaro-oligosaccharides.     

RhoGAPs Attenuate Cell Proliferation by Direct Interaction with p53 Tetramerization Domain

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Effect of Human WEE1 and Stem Cell Factor on Human CD34^＋ Umbilical Cord Blood Cell Damage Induced by Chemotherapeutic Agents

Myelosuppression is one of the major side-effects of most anticancer drugs. To achieve myeloprotection, one bicistronic vector encoding anti-apoptotic protein human WEE l （WEElHu） and proliferation-stimulating stem cell factor （SCF） was generated. In this study, we selected human umbilical cord blood CD34^＋ cells as the in vitro model in an attempt to investigate whether WEEIHu, rather than conventional drug-resistant genes, can be introduced to rescue cells from the damage by chemotherapeutic agents such as cisplatin, adriamycin, mitomycin-c and 5-fluorouracil. Cell viability and cytotoxicity assay, colony-forming units in culture assay and externalization of phospholipid phosphatidylserine analysis showed that the expression of WEElHu and SCF in CD34^＋ cells provided the cells with some protection. These findings suggest that the expression of WEElHu and SCF might rescue CD34^＋ cells from chemotherapyinduced myelosuppression.     

我国高校人力资源开发的问题及其对策

分析了当前我国高校人力资源开发的重要性和存在的主要问题,并运用现代管理理论,提出了树立以人为本的管理理念、建立有利于人才成长的机制、运用柔性化管理模式的措施来促进我国高校人力资源的进一步开发利用。     

数字乳房X片中的伪彩色增强应用

利用数字图像处理中伪彩色理论,讨论了灰度一彩色变换函数,并用合适的线性变换函数对数字乳房X片进行处理。处理后的图像与原数字灰度图像相比较,可分辨性明显提高。可以利用这种变换,辅助医疗诊断,从而降低乳房癌的漏诊和误诊率。经验证,经过伪彩色增强后的数字乳房X片病灶区域的可分辨性明显优于原数字图像,有较高的临床价值。     

口腔内金属材料对磁共振检查的影响

检测口腔内常用金属材料在磁共振检查时是否有伪影和伪影的严重程度。对２１种口腔内常用金属材料做了磁共振成像测试,磁共振仪磁场强度为１．５Ｔ,所用序列是梯度回波。铸金片、银汞合金、银尖等９种材料无伪影;钛合金和金属烤瓷成品有轻度伪影;牙用固位钉、椿钉等１０种材料有严重伪影。部分口腔内金属材料会引起严重伪影,影响图象质量,所以在做口腔颌面部和服部磁共振成像时,须引起重视。     

基于SIFT特征和近似最近邻算法的医学CT图像检索

针对医学X线计算机断层（Computed Tomography,CT）图像,提出了一种基于尺度不变特征变换（Scale InvariantFeature Transform,SIFT）特征和近似最近邻算法的检索方法。首先通过SIFT算法得到图像的特征点和相应的特征向量,再采用近似最近邻算法进行SIFT特征向量的匹配搜索,得到数据库中与参考图像最相似的图像序列。实验结果表明,该法能检索到与目标图像细节相符的结果,大大提高了检索速度。与传统的基于纹理的检索方法相比,查准率和检索结果与目标图像的相似程度方面更佳,符合医学CT图像检索的要求。