全文获取类型
收费全文 | 180049篇 |
免费 | 6965篇 |
国内免费 | 7049篇 |
专业分类
194063篇 |
出版年
2024年 | 203篇 |
2023年 | 1255篇 |
2022年 | 2944篇 |
2021年 | 4873篇 |
2020年 | 3195篇 |
2019年 | 4022篇 |
2018年 | 14748篇 |
2017年 | 12618篇 |
2016年 | 10839篇 |
2015年 | 6385篇 |
2014年 | 7154篇 |
2013年 | 7527篇 |
2012年 | 12386篇 |
2011年 | 19721篇 |
2010年 | 15847篇 |
2009年 | 11972篇 |
2008年 | 14131篇 |
2007年 | 15082篇 |
2006年 | 3780篇 |
2005年 | 3309篇 |
2004年 | 3259篇 |
2003年 | 3107篇 |
2002年 | 2462篇 |
2001年 | 1721篇 |
2000年 | 1499篇 |
1999年 | 1431篇 |
1998年 | 827篇 |
1997年 | 916篇 |
1996年 | 822篇 |
1995年 | 775篇 |
1994年 | 680篇 |
1993年 | 599篇 |
1992年 | 700篇 |
1991年 | 572篇 |
1990年 | 462篇 |
1989年 | 338篇 |
1988年 | 295篇 |
1987年 | 232篇 |
1986年 | 186篇 |
1985年 | 210篇 |
1984年 | 131篇 |
1983年 | 135篇 |
1982年 | 56篇 |
1981年 | 23篇 |
1980年 | 21篇 |
1979年 | 18篇 |
1972年 | 246篇 |
1971年 | 274篇 |
1965年 | 13篇 |
1962年 | 24篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
91.
92.
Osteogenesis imperfecta due to recurrent point mutations at CpG dinucleotides in the COL1A1 gene of type I collagen 总被引:7,自引:3,他引:4
Charles J. Pruchno Daniel H. Cohn Gillian A. Wallis Marcia C. Willing Barbra J. Starman Xiaoming Zhang Peter H. Byers 《Human genetics》1991,87(1):33-40
Summary Most individuals with osteogenesis imperfecta (OI) are heterozygous for dominant mutations in one of the genes that encode the chains of type I collagen. Each of the more than 30 mutations characterized to date has been unique to the affected member (s) of the family. We have determined that two individuals with a progressive deforming variety of OI, OI type III, have the same new dominant mutation [1(I)gly154 to arg] and that two unrelated infants with perinatal lethal OI, OI type II, share a second new dominant muation [1(I)gly1003 to ser]. These mutations occurred at CpG dinucleotides, in a manner consistent with deamination of a methylated cytosine residue, and raise the possibility that CpG dinucleotides are common sites of recurrent mutations in collagen genes. Further, these findings confirm that the OI type-III phenotype, previously thought to be inherited in an autosomal recessive manner, can result from new dominant mutations in the COL1A1 gene of type-I collagen. 相似文献
93.
94.
95.
选用甜椒的小孢子单核期花药,用100、300、500、2000ppm的马来酰肼溶液浸泡处理,并设对照,进行无激素MS固体培养基培养。分别取样用各种组化方法对花药内部多糖、蛋白质、核酸及ATP酶进行组化反应和形态学上的观察。与对照组相比,处理组花药外部形态和内部结构出现许多变异。小孢子内多糖,蛋白质含量减少;绒毡层无明显变化;两组中,核酸的含量均无明显变化;ATP酶的活性低于对照组。可能,马来酰肼对于花药中ATP酶等产生抑制作用,致使花药败育。 相似文献
96.
通过过聚乙二醇6000-磷酸钾缓冲液双相分离、Sephadex G-100凝胶过滤、DEAE-Sephadex A-50离子交换层析、羟基磷灰石层析及SephadexG-100凝胶过滤等提纯步骤,从海枣曲霉(Aspergillus phoenicis)麦麸培养物抽提液中提纯得到凝胶电泳均一的β-半乳糖苷酶。该酶的最适pH为3.5—4.0,最适温度为60℃(反应15分钟),在pH5.0—8.5之间及60℃以下稳定。在65℃和70℃保温时失活50%的时间分别为27和2分钟。用SDS凝胶电泳法和梯度凝胶电泳法分别测得该酶的分子量为115,000和118,000。薄层凝胶等电聚焦法测得其等电点为pH4.6。 相似文献
97.
Morphological changes of sensory CGRP-immunoreactive and sympathetic nerves in peripheral tissues following chronic denervation 总被引:2,自引:0,他引:2
The morphological relationship between sensory and sympathetic nerves was studied in tissues of the eye and the oral cavity following chronic sympathetic or sensory denervation. Immunoreactivities for calcitonin gene-related peptide (CGRP) and tyrosine hydroxylase (TH) were used as indexes to assess the changes of the two nerve populations after denervation. Following surgical sympathectomy, a marked increase of CGRP-containing fibres was seen in all tissues studied, while TH-imunoreactive fibres were totally depleated. Conversely, after capsaicin treatment, an increase of TH-immunoreactive nerves was found in the same tissues, concomitant with a sharp decrease of CGRP-immunoreactive nerves. These changes were particularly evident in iridial stroma and around blood vessels in all tissue, where sensory and sympathetic nerves have a closely overlapping distribution pattern. The altered proportion of sensory peptide- and catecholamine-containing nerves following sympathetic and sensory denervation suggest that there is a reciprocal trophic influence between the two nerve subsets, possibly with the intervention of neurotrophic substances such as nerve growth factor. These results indicate a close interaction between sensory peptidergic and sympathetic nervous systems in peripheral organs. 相似文献
98.
The kinetics of haemolysis of rabbit erythrocytes byCroton tiglium lectin was studied as a function of concentration of the lectin and erythrocytes. The length of the prelytic period decreased with increasing lectin concentrations, indicating that the secondary events at the membrane which follow the binding of the lectin to cell surface carbohydrate receptors are accelerated at higher surface concentrations of the lectin. The rate or extent of haemolysis was not affected by the inclusion of ions like K+, Ca2+ and Mg2+ in the medium or by the substitution of ionic medium by a non-ionic medium. The inhibition of haemagglutination and haemolysis of rabbit red cells byCroton tiglium lectin by antilectin rabbit serum was observed. A possible mechanism of haemolysis by the lectin is discussed. 相似文献
99.
N-Acetyl-D-galactosamine in β-linkage being ubiquitous in cell surface glycoproteins, their interaction with lectins specific for this sugar moiety may be a significant event in cell adhesion phenomena. This article discusses the common β-N-acetyl galactosamine-specific lectins, with particular stress on the lectin from winged beans (Psophocarpus tetragonolobus). 相似文献
100.
Preparation of [B23-D-alanine]des-(B25-B30)-hexapeptide-insulin by a combination of enzymic and non-enzymic synthesis. 下载免费PDF全文
Des-(B25-B30)-hexapeptide-insulin with B23-glycine replaced by D-alanine was prepared by a combination of enzymic and non-enzymic syntheses. The purified product was homogeneous in polyacrylamide-gel electrophoresis and could be crystallized. The biological activity in vivo of crystalline [B23-D-Ala]des-(B25-B30)-hexapeptide-insulin was determined as 58% of that of standard pig insulin (27 i.u./mg). 相似文献