宁夏同心中新世铲齿象化石

本文记述了宁夏同心地区中中新世哺乳动物群中铲齿象化石——同心铲齿象 (Platybelodon tongxinensis) (原定为同心嵌齿象 (Gomphotherium tongxinensis). 通过与国内外已现的有关铲齿象对比,确认其与国内已发现的葛氏种关系最近,可能属同一枝系.同心种与葛氏种相比,是性质相对原始分布层位较低的我国第二个铲齿象种.文章初步讨论了其 M_3 的某些变异性状.并结合现有的其它标本初步阐述了铲齿象类下门齿的两种结构类型,原始型的同心层状结构和衍生型的齿质柱状结构.建议根据这两种结构仍将铲齿象类划分为 Platybelodontinae 和 Amebelodontinae.     

Oxidation-reduction properties of the electron acceptors of Photosystem II I. Redox titration of the flash-induced carotenoid band shift,of C550 and of the variable fluorescence yield in spinach chloroplasts

Redox titration of the electrochromic carotenoid band shift, detected at 50 μs after a saturating actinic flash, in spinach chloroplasts, shows that only one electron acceptor in Photosystem II participates in a transmembrane primary electron transfer. This species, the primary quinone acceptor, Q, shows only one midpoint potential (E_m,7.5) of approx. 0 V and is undoubtedly equivalent to the fluorescence quencher, Q_H. A second titration wave is observed at low potential ($\text{E}{}_{\mathrm{<mtext>m</mtext><mtext>,7.5</mtext>}}\text{? ? 240}\text{mV}$) and at greater than 3 ms after a saturating actinic flash. This wave has an action spectrum different from that of Photosystem II centers containing Q and could arise from a secondary but not primary electron transfer. A low-potential fluorescence quencher is observed in chloroplasts which largely disappears in a single saturating flash at ? 185 mV and which does not participate in a transmembrane electron transfer. This low-potential quencher (probably equivalent to fluorescence quencher, Q_L) and Q are altogether different species. Redox titration of C550 shows that if electron acceptor Q_β is indeed characterized by an E_m,7 of + 120 mV, then this acceptor does not give rise to a C550 signal upon reduction and does not participate in a transmembrane electron transfer. This titration also shows that C550 is not associated with Q_L.     

Oxidation-reduction properties of the electron acceptors of Photosystem II. II. Redox titration at various pH values of the flash-induced formation of C550 in Chlamydomonas Photosystem II particles lacking the secondary quinone electron acceptor

Redox titrations of the flash-induced formation of C550 (a linear indicator of Q^?) were performed between pH 5.9 and 8.3 in Chlamydomonas Photosystem II particles lacking the secondary electron acceptor, B. One-third of the reaction centers show a pH-dependent midpoint potential (E_m,7.5) = ? 30 mV) for redox couple $\text{Q}\text{Q}{}^{\mathrm{?}}$, which varies by ?60 mV/pH unit. Two-thirds of the centers show a pH-independent midpoint potential (E_mm = + 10 mV) for this couple. The elevated pH-independent E_m suggests that in the latter centers the environment of Q has been modified such as to stabilize the semiquinone anion, Q^?. The midpoint potentials of the centers having a pH-dependent E_m are within 20 mV of those observed in chloroplasts having a secondary electron acceptor. It appears therefore that the secondary electron acceptor exerts little influence on the E_m of $\text{Q}\text{Q}{}^{\mathrm{?}}$. An EPR signal at g 1.82 has recently been attributed to a semiquinone-iron complex which comprises Q^?. The similar redox behavior reported here for C550 and reported by others (Evans, M.C.W., Nugent, J.H.A., Tilling, L.A. and Atkinson, Y.E. (1982) FEBS Lett. 145, 176–178) for the g 1.82 signal in similar Photosystem II particles confirm the assignment of this EPR signal to Q^?. At below ?200 mV, illumination of the Photosystem II particles produces an accumulation of reduced pheophytin (Ph^?). At ?420 mV Ph^? appears with a quantum yield of 0.006–0.01 which in this material implies a lifetime of 30–100 ns for the radical pair P-680⁺Ph^?.     

Calorimetric measurement of free energy utilization by Nitrosomonas and Nitrobacter

Summary Calorimetric estimates of the utilization efficiency of the free-energy derived from substrate oxidation by cell suspensions of two nitrifying bacteria, Nitrosomonas and Nitrobacter, provided two ranges of values: 11 to 27% and 15 to 51%, respectively. About 15 to 30% of the utilized free-energy is used for driving endergonic reactions other than CO₂ fixation, probably the synthesis of polyphosphates.The molar heat of substrate oxidation does not seem to be influenced by the age of cells harvested during growth or by the length of the incubation period during which cells have been kept in a buffer suspension in a starved condition. The loss of respiratory activity measured either by oxygen uptake or heat evolution in the presence of the specific substrate, nitrite or ammonium, decreases according to kinetics which are influenced by the aerobiosis of the suspension. The viability of the starved cells decreases in a way which is similar to that of the respiratory activity. It seemed impossible to obtain cells which had lost their viability but kept the ability to oxidize their substrate.Two inhibitors of the respiratory chain, quinacrine and cyanide, are without effect on the molar heat of substrate oxidation and consequently on the free-energy utilization efficiency. 2.4 dinitrophenol did decrease the rate of heat evolution during substrate oxidation at concentrations at which the rate of oxygen uptake was not depressed, with the consequences that free-energy efficiency was apparently increased.     

L'excursion en Provence (Sud-Est de la France) de la Societe Internationale de Phytosociologie

Conclusions La Provence apparaît, en définitive, comme un territoire des plus variés aux divers points de vue climatique, édaphique, biotique.C'est une région d'autant plus favorable aux études phytosociologiques que la variété des milieux s'y double d'une brutalité souvent étonnante des contrastes, conduisant à la juxtaposition parfois extrêmement précise d'associations végétales très différentes, dont les limites peuvent alors être cartographiquement figurées avec une très suffisante exactitude.Cette vigueur des contrastes est surtout marquée en Provence calcaire où les divers termes des séries évolutives dérivant du Quercetum ilicis climacique ou y conduisant, se juxtaposent souvent sans transition de quelque importance.Les contrastes sont cependant moins accusés en Provence cristalline et dans la Basse-Vallée du Rhône.En Crau comme en Camargue, les associations végétales se juxtaposent souvent en ceintures parallèles ou concentriques — elles s'individualisent alors facilement —, souvent en mosaïques irrégulières, au grè des variations locales des deux facteurs déterminants: l'eau en Crau, le sel en Camargue.En Provence cristalline, les Maures étant surtout faites de roches se délitant facilement (grès, micaschistes et phyllades) sous un climat chaud et humide, le sol se reconstitue facilement après sa destruction partielle par l'érosion; les contrastes sont moins tranchés; on observe des transitions nombreuses entre les divers termes des séries évolutives, dont les limites ne peuvent être fixées qu'approximativement. Nous y avons notamment abordé le problème de la forêt de chêne-liège dont la signification est loin d'être connue, non seulement en Provence mais sur toute l'étendue de son aire, en Afrique du Nord, en Espagne, au Portugal, en France méridionale, en Italie et dans les Iles de la Méditerranée occidentale.En provence cristalline, il est certain que l'homme en a considérablement assuré l'extension, directement pour l'utilisation du liège, indirectement par la coupe et le feu, et, en bien des points de la Méditerranée occidentale, le chêne-liège se présente comme une essence cultivée.Par ailleurs rien ne permet de mettre en doute son indigénat. Mais, dans les conditions actuelles du milieu en Provence cristalline:le chêne vert est possible partout;dans les conditions naturelles de la concurrence vitale le chêne vert l'emporte sur le chêne-liège.Si, donc, la forêt originelle a comporté le chêne-liège, ce ne peut être qu'à l'état d'essence secondaire et seulement en quelques points où une faible couverture forestière lui permettait de cohabiter avec le chêne vert.Les documents cartographiques au I/20 000^e tendent à montrer, d'aileurs que, dans les conditions naturelles de reconstitution de la forêt ou dans le peuplement naturel des sols neufs (Isthme de Giens), c'est la chênaie de chêne vert, non la chênaie de chêne-liège qui s'installe.Les grandes unités phytosociologiques de la Provence paraissent être maintenant convenablement définies floristiquement; il convient donc d'en poursuivre l'étude écologique, dont certains aspects seulement les plus apparents—mais peut-être pas toujours les plus actifs-, ont été abordés.Notons enfin la démonstration faite, au cours de l'excursion, de la parfaite application possible des méthodes phytosociologiques à l'étude des groupements marins et la nécessité, particulièrement apparente en milieu marin, de considérer tout autant les animaux que les végétaux. L'avenir est bien aux biocénoses dont les grands traits ont été mis en évidence, quant à la côte de la presqu'île de Giens, par Roger Molinier.Reçu par la rédaction le 20. XII. 1958.avec la collaboration de ROGER MOLINIER pour la partie marine et G. TALLON pour la Camargue.     

普通小麦与簇毛麦双二倍体的合成,育性及细胞遗传学研究

通过杂种幼胚无性系培养获得大量再生植株F_1,经秋水仙碱处理,合成了普通小麦与簇毛麦属间双二倍体(AABBDDVV)。其形态特征除株高、穗长、小穗数,籽粒大小和育性明显增加,生育期延长外,分别与各自的再生植株F_1相似。双二倍体的体细胞染色体数目变化范围为48—56。花粉母细胞减数分裂中期Ⅰ 2n=28Ⅱ的细胞占56.82％,每个细胞平均有27.10个二价体,1.44个单价体,0.08个三价体,0.03个四价体。经过连续两代单穗单株选择,结实率由15.91％提高到36.52％。     

Regulation of glycogenolysis in human skeletal muscle

The role of inorganic phosphate on the regulation of glycogenolysis in resting and contracting muscle was studied in human quadriceps muscle. Increased Pi content was achieved by intermittent electrical stimulation of the muscle followed by occlusion of the blood flow. Occlusion resulted in the maintenance of a high Pi content over a 60-s observation period during which the muscle was either at rest or was stimulated electrically. The study was performed with and without infusion of epinephrine (EPI). In the absence of EPI the phosphorylase a fraction was 50% immediately at the end of the initial stimulation period, declining to 22% after 60 s. With EPI corresponding values for phosphorylase a were 91% initially, 56% after 30 s, and 33% after 60 s, respectively. In both cases the Pi content was increased by approximately 35 mmol/kg dry muscle during the stimulation and remained constant during the occlusion. In neither of these situations was significant degradation of glycogen observed during the occlusion. In the study performed with electrical stimulation during the occlusion period, muscle glycogen degradation was observed both with and without EPI. Phosphorylase a fractions and Pi contents in this study were similar to those observed when muscle was rested over the 60-s occlusion period. The paradox of a high Pi content and extensive transformation of phosphorylase to the a form but low glycogenolytic activity points to additional factors in the regulation of glycogen breakdown.     

八倍体小黑麦×普通小麦杂种后代群体中的染色体易位

用改良的Giemsa C-带技术以单株为基础分析了八倍体小黑麦×普通小麦的杂种BC_1,F_(?)和F_(?)代植株的核型。在鉴定了C-带核型的1098株杂种后代植株中,发现了78条小麦-黑麦和277条黑麦-黑麦易位染色体。在不同的世代和株系中,小麦-黑麦染色体易位率变化在4.35—14.07%之间,平均7.10%;黑麦-黑麦染色体易位率在0.48—52.78%之间,平均25.23%。鉴定的小麦-黑麦易位染色体涉及了黑麦的14条不同的染色体臂和小麦的A、B和D组染色体。易位的48.57%发生在小麦和黑麦的部分同源染色体之间,51.43%发生在非部分同源染色体之间。不同的黑麦染色体臂参与易位的频率不同。小麦-黑麦染色体易位主要发生在杂种的早期世代,使用适当的选择技术在F_3获得了纯合的易位植株。文中讨论了快速选育易位系的技术和它们在小麦育种中的应用问题。     

建兰根状茎增殖条件的研究

研究了建兰两个栽培品种的根状茎在固体及液体两种培养方式,以及培养基中有无激素和不同激素浓度配比对增殖及分化的影响。根状茎在液体培养基上的生长量都大于固体培养基上的生长量。根状茎在无激素的培养基上也能增殖。加入激素有促进作用。合适的激素浓度及配比因品种而异。     

HBsAg重组DNA转化中国地鼠卵巢细胞二氢叶酸还原酶阴性突变株的细胞染色体变化及致瘤性

整合了含乙型肝炎病毒表面抗原(HBsAg)基因和dhfr基因的CHO-dhfr~-细胞,其染色体的畸变率和畸变类型都比亲代CHO-dhfr~-细胞高。但转化前后两系细胞的重要特性都未发生变异,即两者的染色体总数无差别,都是20条。两系细胞株接种裸鼠,均未发现有致瘤性。