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811.
812.
Jingyi Zhu Ivo?H.M. van?Stokkum Laura Paparelli Michael?R. Jones Marie?Louise Groot 《Biophysical journal》2013,104(11):2493-2502
A question at the forefront of biophysical sciences is, to what extent do quantum effects and protein conformational changes play a role in processes such as biological sensing and energy conversion? At the heart of photosynthetic energy transduction lie processes involving ultrafast energy and electron transfers among a small number of tetrapyrrole pigments embedded in the interior of a protein. In the purple bacterial reaction center (RC), a highly efficient ultrafast charge separation takes place between a pair of bacteriochlorophylls: an accessory bacteriochlorophyll (B) and bacteriopheophytin (H). In this work, we applied ultrafast spectroscopy in the visible and near-infrared spectral region to Rhodobacter sphaeroides RCs to accurately track the timing of the electron on BA and HA via the appearance of the BA and HA anion bands. We observed an unexpectedly early rise of the HA− band that challenges the accepted simple picture of stepwise electron transfer with 3 ps and 1 ps time constants. The implications for the mechanism of initial charge separation in bacterial RCs are discussed in terms of a possible adiabatic electron transfer step between BA and HA, and the effect of protein conformation on the electron transfer rate. 相似文献
813.
Mei-Rong Long Dao-Feng Zhang Xin-Yi Yang Xiao-Mei Zhang Yong-Guang Zhang Yuan-Ming Zhang Honghui Zhu Wen-Jun Li 《Antonie van Leeuwenhoek》2013,103(5):997-1005
A novel Gram-negative, aerobic, slightly halophilic, yellow-pigmented, oxidase-negative, Voges–Proskauer positive, non-spore-forming bacterium, designated YIM M 13059T, was isolated from a sediment sample collected from the South China Sea at a depth of 310 m. Optimal growth was found to occur at 28–30 °C, pH 7.0 and in the presence of 3–4 % (w/v) NaCl. Cells were observed to be rod-shaped and motile by peritrichous flagella. The polar lipids of strain YIM M 13059T were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a ninhydrin-positive phospholipid, one glycolipid and two unknown phospholipids. The predominant respiratory quinone was determined to be Q-9. The major fatty acids were identified as C18:1 ω7c, C16:1 ω6c/C16:1 ω7c, C16:0 and C12:0 3-OH. The genomic DNA G+C content was determined to be 54.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate belongs to the genus Halomonas in the family Halomonadaceae. The 16S rRNA gene sequence similarities between strain YIM M 13059 T and the type strains of members of the genus Halomonas were in the range 93.3–98.3 %. However, the levels of DNA–DNA relatedness values between YIM M 13059 and the type strains of the most closely related species, Halomonas zhangjiangensis, Halomonas variabilis, Halomonas neptunia, Halomonas boliviensis and Halomonas sulfadieris were 50.2 ± 0.68 %, 46.8 ± 1.9 %, 28.5 ± 0.74 %, 42.9 ± 0.55 % and 37.1 ± 0.68 %, respectively. Based on phylogenetic, chemotaxonomic and phenotypic data, the strain YIM M 13059T is proposed to represent a novel member of the genus Halomonas, with the name Halomonas nanhaiensis sp. nov. The type strain is YIM M 13059T (=JCM 18142T =CCTCC AB 2012911T). 相似文献
814.
815.
Guoguo Zhu Yingcheng Zheng Lianglu Zhang Yingying Shi Wenhua Li Zhongchun Liu Biwen Peng Jun Yin Wanhong Liu Xiaohua He 《Biochemical and biophysical research communications》2013
Coxsackievirus A16 (CA16) infection, which is responsible for hand, foot and mouth disease (HFMD), has become a common health problem in Asia due to the prevalence of the virus. Thus, it is important to understand the pathogenesis of CA16 infection. Viruses that induce endoplasmic reticulum (ER) stress are confronted with the unfolded protein response (UPR), which may lead to apoptotic cell death and influence viral replication. In this study, we found that CA16 infection could induce apoptosis and ER stress in RD cells. Interestingly, apoptosis via the activation of caspase-3, -8 and -9 in the extrinsic or intrinsic apoptotic pathways in RD cells was inhibited by 4-phenyl butyric acid (4PBA), a chemical chaperone that reduces ER stress. These results suggest that CA16 infection leads to ER stress, which in turn results in prolonged ER stress-induced apoptosis. This study provides a new basis for understanding CA16 infection and host responses. 相似文献
816.
Xiao Wang Zhong-Wei Zhang Shi-Hua Tu Wen-Qiang Feng Fei Xu Feng Zhu Da-Wei Zhang Jun-Bo Du Shu Yuan Hong-Hui Lin 《Biologia》2013,68(1):74-81
Cadmium (Cd) has been identified as a significant pollutant due to its high solubility in water and soil and high toxicity to plants and animals. Rice, as one of the most important food crops, is grown in soils with variable levels of Cd and therefore, is important to discriminate the Cd tolerance of different rice cultivars to determine their suitability for cultivation in Cd-contaminated soils. This study investigates the primary mechanisms employed by four rice cultivars in attaining Cd tolerance. HA63 cultivar reduces Cd uptake by increasing Fe absorption through activation of phytosiderophores. T3028 cultivar accumulates the highest level of Cd in leaves while also activating its reactive oxygen species (ROS) scavenging system, including antioxidant enzymes and phytochelatins. In some rice cultivars (such as HA63), a cyanide-resistant respiration mechanism, important in Cd detoxification, was also promoted under the Cd stress. In conclusion, different rice cultivars may adopt different biochemical strategies and respond with different efficiency to Cd stress. 相似文献
817.
Ling Xu Xiujuan Qu Xuejun Hu Zhitu Zhu Ce Li Enze Li Yanju Ma Na Song Yunpeng Liu 《FEBS letters》2013
Gastric cancer cells are resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and the resistance mechanism is not fully understood. In human gastric cancer MGC803 and BGC823 cells, TRAIL induces insulin-like growth factor-1 receptor (IGF-1R) pathway activation. Treatment with IGF-1R inhibitor OSI-906 or small interfering RNAs against IGF-1R, prevents IGF-1R pathway activation and increases TRAIL-induced apoptosis. The TRAIL-induced IGF-1R pathway activation is promoted by IGF-1R translocation into lipid rafts. Moreover, the translocation of IGF-1R into lipid rafts is regulated by Casitas B-lineage lymphoma b (Cbl-b). Taken together, TRAIL-induced IGF-1R activation antagonizes TRAIL-induced apoptosis by Cbl-b-regulated distribution of IGF-1R in lipid rafts. 相似文献
818.
The purpose of this microcosm experiment was to determine whether the freshwater snail Bellamya aeruginosa affected phytoplankton community and water quality. Three treatments of different snail densities (low, medium, and high) and a control (no snails) were set up in twelve enclosures. Chlorophyll a (chl a), transparency (SD), water temperature (WT), dissolved oxygen (DO), pH, nutrients, and abundance of phytoplankton were determined on days 0, 3, 7, 11, 15, 23, 31, 46, and 61. The total chl a concentration decreased and SD increased in the treatments, whereas the proportion of cyanobacteria chl a increased. Bellamya seemed to result indirectly in a decrease of DO, by reducing the algal biomass rather than by respiration. A significant decrease in nitrogen–phosphorus ratios was observed in the treatments. In the enclosed system the abundance of colonial green algae decreased over time whereas that of cyanobacteria and flagellates increased. Principal response curves showed that both phytoplankton community and water quality in the medium and high-density treatments were consistently different from in the control. These results indicate that the presence of snails resulted in a significant change of water physicochemical properties and phytoplankton community. 相似文献
819.
We examined crustacean zooplankton data (excluding nauplii) from 15 shallow lakes in south and central Florida, spanning a range of sizes and Chlorophyll-a concentrations. Each dataset was comprised of monthly samples from 2 years (6 lakes), or monthly to quarterly samples from 10 or 12 years (9 lakes). We quantified relationships between the zooplankton to phytoplankton biomass ratio (BZ:BP) and measurements of BZ and BP at three levels of resolution: (1) sampling events; (2) seasonal means; and (3) period-of-record means and medians. For individual sampling events, variations in ZB explained most of the variation in BZ:BP and ratios were little affected by changes in BP. Seasonal declines in BZ:BP corresponded with declines in BZ, were not related to declines in biomass of edible algae, and happened in spring–summer when earlier studies indicated high densities of planktivorous fish. Period-of-record means and medians did not identify any relationships between the biomass ratio and either BZ or BP, suggesting that processes affecting the ratio operate at shorter time scales than multiple years. Short-term and seasonal changes in BZ:BP in Florida may be controlled by predation. Testing this hypothesis will require coincident sampling of plankton and fish over a number of years or experimental studies. 相似文献
820.
Xiaoyan Cheng Yan Wu Jianping Guo Bo Du Rongzhi Chen Lili Zhu Guangcun He 《The Plant journal : for cell and molecular biology》2013,76(4):687-698
Seed germination and innate immunity both have significant effects on plant life spans because they control the plant's entry into the ecosystem and provide defenses against various external stresses, respectively. Much ecological evidence has shown that seeds with high vigor are generally more tolerant of various environmental stimuli in the field than those with low vigor. However, there is little genetic evidence linking germination and immunity in plants. Here, we show that the rice lectin receptor‐like kinase OslecRK contributes to both seed germination and plant innate immunity. We demonstrate that knocking down the OslecRK gene depresses the expression of α–amylase genes, reducing seed viability and thereby decreasing the rate of seed germination. Moreover, it also inhibits the expression of defense genes, and so reduces the resistance of rice plants to fungal and bacterial pathogens as well as herbivorous insects. Yeast two‐hybrid and co‐immunoprecipitation experiments revealed that OslecRK interacts with an actin‐depolymerizing factor (ADF) in vivo via its kinase domain. Moreover, the rice adf mutant exhibited a reduced seed germination rate due to the suppression of α–amylase gene expression. This mutant also exhibited depressed immune responses and reduced resistance to biotic stresses. Our results thus provide direct genetic evidence for a common physiological pathway connecting germination and immunity in plants. They also partially explain the common observation that high‐vigor seeds often perform well in the field. The dual effects of OslecRK may be indicative of progressive adaptive evolution in rice. 相似文献